Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)

Autores
Medina, Rocío; Lucentini, César Gustavo; Franco, Mario Emilio Ernesto; Petroselli, Gabriela; Rosso, Janina Alejandra; Erra-Balsells, Rosa; Balatti, Pedro Alberto; Saparrat, Mario
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8- dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
Materia
Ciencias Químicas
Biochemistry
Genetics
Microbiology
Molecular biology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/8757
Acceder
id CICBA_669e8d89dc3272afabb33e0ddf327a5a
oai_identifier_str oai:digital.cic.gba.gob.ar:11746/8757
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)Medina, RocíoLucentini, César GustavoFranco, Mario Emilio ErnestoPetroselli, GabrielaRosso, Janina AlejandraErra-Balsells, RosaBalatti, Pedro AlbertoSaparrat, MarioCiencias QuímicasBiochemistryGeneticsMicrobiologyMolecular biologyFulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8- dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.2018-12-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8757enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:43:21Zoai:digital.cic.gba.gob.ar:11746/8757Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:43:21.694CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
spellingShingle Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
Medina, Rocío
Ciencias Químicas
Biochemistry
Genetics
Microbiology
Molecular biology
title_short Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_fullStr Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full_unstemmed Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_sort Identification of an intermediate for 1,8- dihydroxynaphthalene melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
dc.creator.none.fl_str_mv Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra-Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario
author Medina, Rocío
author_facet Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra-Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario
author_role author
author2 Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra-Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Químicas
Biochemistry
Genetics
Microbiology
Molecular biology
topic Ciencias Químicas
Biochemistry
Genetics
Microbiology
Molecular biology
dc.description.none.fl_txt_mv Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8- dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
description Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8- dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/8757
url https://digital.cic.gba.gob.ar/handle/11746/8757
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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score 12.623145

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