<i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina

Autores
Rosso, Lorena Beatriz; Sedan, Daniela Yazmine; Kolman, María de los Ángeles; Caixach, Josep; Flores, Cintia; Oteiza, Juan Manuel; Salerno, Graciela Lidia; Echenique, Ricardo Omar; Giannuzzi, Leda; Andrinolo, Darío
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
Facultad de Ciencias Exactas
Facultad de Ciencias Naturales y Museo
Materia
Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/97702
Acceder
id SEDICI_9a54ea47d71b4e728ae14bacef515933
oai_identifier_str oai:sedici.unlp.edu.ar:10915/97702
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, ArgentinaRosso, Lorena BeatrizSedan, Daniela YazmineKolman, María de los ÁngelesCaixach, JosepFlores, CintiaOteiza, Juan ManuelSalerno, Graciela LidiaEchenique, Ricardo OmarGiannuzzi, LedaAndrinolo, DaríoBiologíaCyanobacteriaMicrocystis aeruginosa[d-leu1] microcystin-lr [m+h]+ArgentinaHarmful algal bloomsObjective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.Facultad de Ciencias ExactasFacultad de Ciencias Naturales y Museo2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf287-296http://sedici.unlp.edu.ar/handle/10915/97702enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/10936info:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2014-0002info:eu-repo/semantics/altIdentifier/hdl/11336/10936info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:55Zoai:sedici.unlp.edu.ar:10915/97702Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:55.682SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
spellingShingle <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
Rosso, Lorena Beatriz
Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
title_short <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_full <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_fullStr <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_full_unstemmed <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_sort <i>Microcystis aeruginos</i> strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
dc.creator.none.fl_str_mv Rosso, Lorena Beatriz
Sedan, Daniela Yazmine
Kolman, María de los Ángeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Manuel
Salerno, Graciela Lidia
Echenique, Ricardo Omar
Giannuzzi, Leda
Andrinolo, Darío
author Rosso, Lorena Beatriz
author_facet Rosso, Lorena Beatriz
Sedan, Daniela Yazmine
Kolman, María de los Ángeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Manuel
Salerno, Graciela Lidia
Echenique, Ricardo Omar
Giannuzzi, Leda
Andrinolo, Darío
author_role author
author2 Sedan, Daniela Yazmine
Kolman, María de los Ángeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Manuel
Salerno, Graciela Lidia
Echenique, Ricardo Omar
Giannuzzi, Leda
Andrinolo, Darío
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
topic Biología
Cyanobacteria
Microcystis aeruginosa
[d-leu1] microcystin-lr [m+h]+
Argentina
Harmful algal blooms
dc.description.none.fl_txt_mv Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
Facultad de Ciencias Exactas
Facultad de Ciencias Naturales y Museo
description Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/97702
url http://sedici.unlp.edu.ar/handle/10915/97702
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/10936
info:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2014-0002
info:eu-repo/semantics/altIdentifier/hdl/11336/10936
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
287-296
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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