Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina

Autores
Rosso, Lorena; Sedan, Daniela Yazmine; Kolman, Maria de Los Angeles; Caixach, Josep; Flores, Cintia; Oteiza, Juan Martín; Salerno, Graciela Lidia; Echenique, Ricardo; Giannuzzi, Leda; Andrinolo, Dario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
Fil: Rosso, Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Sedan, Daniela Yazmine. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kolman, Maria de Los Angeles. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caixach, Josep. Institut de Diagnosi Ambiental i Estudis de l'Aigua; España
Fil: Flores, Cintia. Institut de Diagnosi Ambiental i Estudis de l'Aigua; España
Fil: Oteiza, Juan Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echenique, Ricardo. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficologia; Argentina
Fil: Giannuzzi, Leda. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Andrinolo, Dario. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cyanobacteria
Microcystis aeruginosa
[D-Leu1] microcystin-LR [M+H]+
Argentina
Harmful algal blooms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10936
Acceder
id CONICETDig_d857396c3b0ff5eb2b9c7c324780b960
oai_identifier_str oai:ri.conicet.gov.ar:11336/10936
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, ArgentinaRosso, LorenaSedan, Daniela YazmineKolman, Maria de Los AngelesCaixach, JosepFlores, CintiaOteiza, Juan MartínSalerno, Graciela LidiaEchenique, RicardoGiannuzzi, LedaAndrinolo, DarioCyanobacteriaMicrocystis aeruginosa[D-Leu1] microcystin-LR [M+H]+ArgentinaHarmful algal bloomshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.Fil: Rosso, Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Sedan, Daniela Yazmine. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kolman, Maria de Los Angeles. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caixach, Josep. Institut de Diagnosi Ambiental i Estudis de l'Aigua; EspañaFil: Flores, Cintia. Institut de Diagnosi Ambiental i Estudis de l'Aigua; EspañaFil: Oteiza, Juan Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Echenique, Ricardo. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficologia; ArgentinaFil: Giannuzzi, Leda. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Andrinolo, Dario. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaJournal of Coastal Life Medicine Editorial2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10936Rosso, Lorena; Sedan, Daniela Yazmine; Kolman, Maria de Los Angeles; Caixach, Josep; Flores, Cintia; et al.; Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina; Journal of Coastal Life Medicine Editorial; Journal of Coastal Life Medicine; 2; 4; 3-2014; 287-2962309-52882309-6152enginfo:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2014-0002info:eu-repo/semantics/altIdentifier/url/http://www.jclmm.com/qk/20144/7.pdfinfo:eu-repo/semantics/altIdentifier/url/https://doaj.org/article/106c7338b85547e297d7f7cc71796ebbinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:06:05Zoai:ri.conicet.gov.ar:11336/10936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:06:05.65CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
spellingShingle Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
Rosso, Lorena
Cyanobacteria
Microcystis aeruginosa
[D-Leu1] microcystin-LR [M+H]+
Argentina
Harmful algal blooms
title_short Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_full Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_fullStr Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_full_unstemmed Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
title_sort Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina
dc.creator.none.fl_str_mv Rosso, Lorena
Sedan, Daniela Yazmine
Kolman, Maria de Los Angeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Martín
Salerno, Graciela Lidia
Echenique, Ricardo
Giannuzzi, Leda
Andrinolo, Dario
author Rosso, Lorena
author_facet Rosso, Lorena
Sedan, Daniela Yazmine
Kolman, Maria de Los Angeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Martín
Salerno, Graciela Lidia
Echenique, Ricardo
Giannuzzi, Leda
Andrinolo, Dario
author_role author
author2 Sedan, Daniela Yazmine
Kolman, Maria de Los Angeles
Caixach, Josep
Flores, Cintia
Oteiza, Juan Martín
Salerno, Graciela Lidia
Echenique, Ricardo
Giannuzzi, Leda
Andrinolo, Dario
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cyanobacteria
Microcystis aeruginosa
[D-Leu1] microcystin-LR [M+H]+
Argentina
Harmful algal blooms
topic Cyanobacteria
Microcystis aeruginosa
[D-Leu1] microcystin-LR [M+H]+
Argentina
Harmful algal blooms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
Fil: Rosso, Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Sedan, Daniela Yazmine. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kolman, Maria de Los Angeles. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caixach, Josep. Institut de Diagnosi Ambiental i Estudis de l'Aigua; España
Fil: Flores, Cintia. Institut de Diagnosi Ambiental i Estudis de l'Aigua; España
Fil: Oteiza, Juan Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echenique, Ricardo. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficologia; Argentina
Fil: Giannuzzi, Leda. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Andrinolo, Dario. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective: To show the toxicological and phylogenetic characterization of a native Microcystis aeruginosa (M. aeruginosa) strain (named CAAT 2005-3) isolated from a water body of Buenos Aires province, Argentine. Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped with an electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESI-HRMS microcystins analysis. The number of cell/mL and [D-Leu1 ] Mcyst-LR production obtained as a function of time was modelled using the Gompertz equation. Results: The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI. The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT 2005-3 expresses a toxin variant, that was identified by LC-HRMS/Orbitrapas as [D-Leu1 ] microcystin-LR ([M+H]+=1037.8 m/z). Conclusions: [D-Leu1 ] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10936
Rosso, Lorena; Sedan, Daniela Yazmine; Kolman, Maria de Los Angeles; Caixach, Josep; Flores, Cintia; et al.; Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina; Journal of Coastal Life Medicine Editorial; Journal of Coastal Life Medicine; 2; 4; 3-2014; 287-296
2309-5288
2309-6152
url http://hdl.handle.net/11336/10936
identifier_str_mv Rosso, Lorena; Sedan, Daniela Yazmine; Kolman, Maria de Los Angeles; Caixach, Josep; Flores, Cintia; et al.; Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina; Journal of Coastal Life Medicine Editorial; Journal of Coastal Life Medicine; 2; 4; 3-2014; 287-296
2309-5288
2309-6152
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2014-0002
info:eu-repo/semantics/altIdentifier/url/http://www.jclmm.com/qk/20144/7.pdf
info:eu-repo/semantics/altIdentifier/url/https://doaj.org/article/106c7338b85547e297d7f7cc71796ebb
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
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application/pdf
dc.publisher.none.fl_str_mv Journal of Coastal Life Medicine Editorial
publisher.none.fl_str_mv Journal of Coastal Life Medicine Editorial
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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