Physical and genetic map of Epinotia aporema granulovirus genome

Autores
Parola, Alejandro Daniel; Manzán, María Alejandra; Lozano, Mario Enrique; Ghiringhelli, P. Daniel; Sciocco-Cap, Alicia; Romanowski, Víctor
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.
Este documento tiene una corrección (ver documento relacionado).
Instituto de Biotecnología y Biología Molecular
Materia
Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145299

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spelling Physical and genetic map of Epinotia aporema granulovirus genomeParola, Alejandro DanielManzán, María AlejandraLozano, Mario EnriqueGhiringhelli, P. DanielSciocco-Cap, AliciaRomanowski, VíctorBiologíaBaculovirusEpapGVEpinotia aporemaGranulinGranulovirusPhylogenyThe bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.Este documento tiene una corrección (ver documento relacionado).Instituto de Biotecnología y Biología Molecular2002info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf329-341http://sedici.unlp.edu.ar/handle/10915/145299enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994Xinfo:eu-repo/semantics/altIdentifier/doi/10.1023/a:1020992412175info:eu-repo/semantics/altIdentifier/pmid/12881644info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/145299Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.147SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Physical and genetic map of Epinotia aporema granulovirus genome
title Physical and genetic map of Epinotia aporema granulovirus genome
spellingShingle Physical and genetic map of Epinotia aporema granulovirus genome
Parola, Alejandro Daniel
Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
title_short Physical and genetic map of Epinotia aporema granulovirus genome
title_full Physical and genetic map of Epinotia aporema granulovirus genome
title_fullStr Physical and genetic map of Epinotia aporema granulovirus genome
title_full_unstemmed Physical and genetic map of Epinotia aporema granulovirus genome
title_sort Physical and genetic map of Epinotia aporema granulovirus genome
dc.creator.none.fl_str_mv Parola, Alejandro Daniel
Manzán, María Alejandra
Lozano, Mario Enrique
Ghiringhelli, P. Daniel
Sciocco-Cap, Alicia
Romanowski, Víctor
author Parola, Alejandro Daniel
author_facet Parola, Alejandro Daniel
Manzán, María Alejandra
Lozano, Mario Enrique
Ghiringhelli, P. Daniel
Sciocco-Cap, Alicia
Romanowski, Víctor
author_role author
author2 Manzán, María Alejandra
Lozano, Mario Enrique
Ghiringhelli, P. Daniel
Sciocco-Cap, Alicia
Romanowski, Víctor
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
topic Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
dc.description.none.fl_txt_mv The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.
Este documento tiene una corrección (ver documento relacionado).
Instituto de Biotecnología y Biología Molecular
description The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.
publishDate 2002
dc.date.none.fl_str_mv 2002
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145299
url http://sedici.unlp.edu.ar/handle/10915/145299
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0920-8569
info:eu-repo/semantics/altIdentifier/issn/1572-994X
info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1020992412175
info:eu-repo/semantics/altIdentifier/pmid/12881644
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
329-341
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