Physical and genetic map of Epinotia aporema granulovirus genome
- Autores
- Parola, Alejandro Daniel; Manzán, María Alejandra; Lozano, Mario Enrique; Ghiringhelli, P. Daniel; Sciocco-Cap, Alicia; Romanowski, Víctor
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.
Este documento tiene una corrección (ver documento relacionado).
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/145299
Ver los metadatos del registro completo
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Physical and genetic map of Epinotia aporema granulovirus genomeParola, Alejandro DanielManzán, María AlejandraLozano, Mario EnriqueGhiringhelli, P. DanielSciocco-Cap, AliciaRomanowski, VíctorBiologíaBaculovirusEpapGVEpinotia aporemaGranulinGranulovirusPhylogenyThe bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.Este documento tiene una corrección (ver documento relacionado).Instituto de Biotecnología y Biología Molecular2002info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf329-341http://sedici.unlp.edu.ar/handle/10915/145299enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994Xinfo:eu-repo/semantics/altIdentifier/doi/10.1023/a:1020992412175info:eu-repo/semantics/altIdentifier/pmid/12881644info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/145299Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.147SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Physical and genetic map of Epinotia aporema granulovirus genome |
title |
Physical and genetic map of Epinotia aporema granulovirus genome |
spellingShingle |
Physical and genetic map of Epinotia aporema granulovirus genome Parola, Alejandro Daniel Biología Baculovirus EpapGV Epinotia aporema Granulin Granulovirus Phylogeny |
title_short |
Physical and genetic map of Epinotia aporema granulovirus genome |
title_full |
Physical and genetic map of Epinotia aporema granulovirus genome |
title_fullStr |
Physical and genetic map of Epinotia aporema granulovirus genome |
title_full_unstemmed |
Physical and genetic map of Epinotia aporema granulovirus genome |
title_sort |
Physical and genetic map of Epinotia aporema granulovirus genome |
dc.creator.none.fl_str_mv |
Parola, Alejandro Daniel Manzán, María Alejandra Lozano, Mario Enrique Ghiringhelli, P. Daniel Sciocco-Cap, Alicia Romanowski, Víctor |
author |
Parola, Alejandro Daniel |
author_facet |
Parola, Alejandro Daniel Manzán, María Alejandra Lozano, Mario Enrique Ghiringhelli, P. Daniel Sciocco-Cap, Alicia Romanowski, Víctor |
author_role |
author |
author2 |
Manzán, María Alejandra Lozano, Mario Enrique Ghiringhelli, P. Daniel Sciocco-Cap, Alicia Romanowski, Víctor |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Biología Baculovirus EpapGV Epinotia aporema Granulin Granulovirus Phylogeny |
topic |
Biología Baculovirus EpapGV Epinotia aporema Granulin Granulovirus Phylogeny |
dc.description.none.fl_txt_mv |
The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation. Este documento tiene una corrección (ver documento relacionado). Instituto de Biotecnología y Biología Molecular |
description |
The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://sedici.unlp.edu.ar/handle/10915/145299 |
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http://sedici.unlp.edu.ar/handle/10915/145299 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0920-8569 info:eu-repo/semantics/altIdentifier/issn/1572-994X info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1020992412175 info:eu-repo/semantics/altIdentifier/pmid/12881644 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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