Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain

Autores
Salerno, María Isabel; Gianinazzi, Silvio; Arnould, Christine; Gianinazzi-Pearson, Vivienne
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium species are soil-borne fungal pathogens that produce a variety of disease symptoms when attacking crop plants. The mode of root colonization of Eucalyptus viminalis seedlings by a pathogenic F. oxyporum strain (Foeu1) at the ultrastructural level and changes in cell wall pectin during host pathogen interactions are described. Root systems of E. viminalis plants were inoculated with F. oxysporum in an in vitro model system. Hyphae of F. oxysporum adhered to the outer epidermal cell walls through fibrillar material, and after penetration they spread into the internal tissues. They developed intercellularly and intracellularly in the root cortex and invaded vascular tissues. Papillae were induced, and the host plasma membrane ruptured in colonized cells, causing rapid host tissue and cell damage. Changes in distribution and occurrence of nonesterified and methyl-esterified pectins were evaluated after root colonization by F. oxysporum using two monoclonal antibodies, JIM 5 and JIM 7, respectively. Nonesterified pectin in control roots was mainly localized in the epidermal cell walls and middle lamellae in parenchymal cortex, whereas methyl-esterified pectin accumulated more in primary cell walls of the cortex and phloem. Decreases in immunodetected nonesterified and methyl-esterified pectins were associated with extensive plant tissue degradation after root colonization by the pathogenic fungus.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Root pathogenesis
Fusarium oxysporum
Eucalyptus viminalis
Ultrastructure
Pectin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/141454

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network_name_str SEDICI (UNLP)
spelling Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strainSalerno, María IsabelGianinazzi, SilvioArnould, ChristineGianinazzi-Pearson, VivienneCiencias AgrariasRoot pathogenesisFusarium oxysporumEucalyptus viminalisUltrastructurePectin<i>Fusarium</i> species are soil-borne fungal pathogens that produce a variety of disease symptoms when attacking crop plants. The mode of root colonization of <i>Eucalyptus viminalis</i> seedlings by a pathogenic <i>F. oxyporum</i> strain (Foeu1) at the ultrastructural level and changes in cell wall pectin during host pathogen interactions are described. Root systems of <i>E. viminalis</i> plants were inoculated with <i>F. oxysporum</i> in an in vitro model system. Hyphae of <i>F. oxysporum</i> adhered to the outer epidermal cell walls through fibrillar material, and after penetration they spread into the internal tissues. They developed intercellularly and intracellularly in the root cortex and invaded vascular tissues. Papillae were induced, and the host plasma membrane ruptured in colonized cells, causing rapid host tissue and cell damage. Changes in distribution and occurrence of nonesterified and methyl-esterified pectins were evaluated after root colonization by <i>F. oxysporum</i> using two monoclonal antibodies, JIM 5 and JIM 7, respectively. Nonesterified pectin in control roots was mainly localized in the epidermal cell walls and middle lamellae in parenchymal cortex, whereas methyl-esterified pectin accumulated more in primary cell walls of the cortex and phloem. Decreases in immunodetected nonesterified and methyl-esterified pectins were associated with extensive plant tissue degradation after root colonization by the pathogenic fungus.Facultad de Ciencias Agrarias y Forestales2004-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf145-152http://sedici.unlp.edu.ar/handle/10915/141454enginfo:eu-repo/semantics/altIdentifier/issn/1345-2630info:eu-repo/semantics/altIdentifier/issn/1610-739Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10327-004-0107-xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:26Zoai:sedici.unlp.edu.ar:10915/141454Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:27.117SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
title Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
spellingShingle Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
Salerno, María Isabel
Ciencias Agrarias
Root pathogenesis
Fusarium oxysporum
Eucalyptus viminalis
Ultrastructure
Pectin
title_short Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
title_full Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
title_fullStr Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
title_full_unstemmed Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
title_sort Ultrastructural and cell wall modifications during infection of <i>Eucalyptus viminalis</i> roots by a pathogenic <i>Fusarium oxysporum</i> strain
dc.creator.none.fl_str_mv Salerno, María Isabel
Gianinazzi, Silvio
Arnould, Christine
Gianinazzi-Pearson, Vivienne
author Salerno, María Isabel
author_facet Salerno, María Isabel
Gianinazzi, Silvio
Arnould, Christine
Gianinazzi-Pearson, Vivienne
author_role author
author2 Gianinazzi, Silvio
Arnould, Christine
Gianinazzi-Pearson, Vivienne
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Root pathogenesis
Fusarium oxysporum
Eucalyptus viminalis
Ultrastructure
Pectin
topic Ciencias Agrarias
Root pathogenesis
Fusarium oxysporum
Eucalyptus viminalis
Ultrastructure
Pectin
dc.description.none.fl_txt_mv <i>Fusarium</i> species are soil-borne fungal pathogens that produce a variety of disease symptoms when attacking crop plants. The mode of root colonization of <i>Eucalyptus viminalis</i> seedlings by a pathogenic <i>F. oxyporum</i> strain (Foeu1) at the ultrastructural level and changes in cell wall pectin during host pathogen interactions are described. Root systems of <i>E. viminalis</i> plants were inoculated with <i>F. oxysporum</i> in an in vitro model system. Hyphae of <i>F. oxysporum</i> adhered to the outer epidermal cell walls through fibrillar material, and after penetration they spread into the internal tissues. They developed intercellularly and intracellularly in the root cortex and invaded vascular tissues. Papillae were induced, and the host plasma membrane ruptured in colonized cells, causing rapid host tissue and cell damage. Changes in distribution and occurrence of nonesterified and methyl-esterified pectins were evaluated after root colonization by <i>F. oxysporum</i> using two monoclonal antibodies, JIM 5 and JIM 7, respectively. Nonesterified pectin in control roots was mainly localized in the epidermal cell walls and middle lamellae in parenchymal cortex, whereas methyl-esterified pectin accumulated more in primary cell walls of the cortex and phloem. Decreases in immunodetected nonesterified and methyl-esterified pectins were associated with extensive plant tissue degradation after root colonization by the pathogenic fungus.
Facultad de Ciencias Agrarias y Forestales
description <i>Fusarium</i> species are soil-borne fungal pathogens that produce a variety of disease symptoms when attacking crop plants. The mode of root colonization of <i>Eucalyptus viminalis</i> seedlings by a pathogenic <i>F. oxyporum</i> strain (Foeu1) at the ultrastructural level and changes in cell wall pectin during host pathogen interactions are described. Root systems of <i>E. viminalis</i> plants were inoculated with <i>F. oxysporum</i> in an in vitro model system. Hyphae of <i>F. oxysporum</i> adhered to the outer epidermal cell walls through fibrillar material, and after penetration they spread into the internal tissues. They developed intercellularly and intracellularly in the root cortex and invaded vascular tissues. Papillae were induced, and the host plasma membrane ruptured in colonized cells, causing rapid host tissue and cell damage. Changes in distribution and occurrence of nonesterified and methyl-esterified pectins were evaluated after root colonization by <i>F. oxysporum</i> using two monoclonal antibodies, JIM 5 and JIM 7, respectively. Nonesterified pectin in control roots was mainly localized in the epidermal cell walls and middle lamellae in parenchymal cortex, whereas methyl-esterified pectin accumulated more in primary cell walls of the cortex and phloem. Decreases in immunodetected nonesterified and methyl-esterified pectins were associated with extensive plant tissue degradation after root colonization by the pathogenic fungus.
publishDate 2004
dc.date.none.fl_str_mv 2004-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141454
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dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/1610-739X
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10327-004-0107-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
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