Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis

Autores
Serra, Diego Omar; Lücking, Genia; Weiland, Florian; Schulz, Stefan; Görg, Angelika; Yantorno, Osvaldo Miguel; Ehling Schulz, Monika
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in Bordetella pertussis, the agent responsible for whooping cough. Recent reports indicate that B. pertussis adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of B. pertussis, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on B. pertussis physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of B. pertussis life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on B. pertussis pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/84205
Acceder
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network_name_str SEDICI (UNLP)
spelling Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussisSerra, Diego OmarLücking, GeniaWeiland, FlorianSchulz, StefanGörg, AngelikaYantorno, Osvaldo MiguelEhling Schulz, MonikaCiencias ExactasBiofilmBordetella pertussisFT-IR spectroscopyProteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of <i>B. pertussis</i>, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on <i>B. pertussis</i> physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of <i>B. pertussis</i> life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on <i>B. pertussis</i> pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf4995-5010http://sedici.unlp.edu.ar/handle/10915/84205enginfo:eu-repo/semantics/altIdentifier/issn/1615-9853info:eu-repo/semantics/altIdentifier/doi/10.1002/pmic.200800218info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:48:31Zoai:sedici.unlp.edu.ar:10915/84205Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:48:31.428SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
spellingShingle Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
Serra, Diego Omar
Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
title_short Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_full Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_fullStr Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_full_unstemmed Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_sort Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
dc.creator.none.fl_str_mv Serra, Diego Omar
Lücking, Genia
Weiland, Florian
Schulz, Stefan
Görg, Angelika
Yantorno, Osvaldo Miguel
Ehling Schulz, Monika
author Serra, Diego Omar
author_facet Serra, Diego Omar
Lücking, Genia
Weiland, Florian
Schulz, Stefan
Görg, Angelika
Yantorno, Osvaldo Miguel
Ehling Schulz, Monika
author_role author
author2 Lücking, Genia
Weiland, Florian
Schulz, Stefan
Görg, Angelika
Yantorno, Osvaldo Miguel
Ehling Schulz, Monika
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
topic Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
dc.description.none.fl_txt_mv Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of <i>B. pertussis</i>, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on <i>B. pertussis</i> physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of <i>B. pertussis</i> life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on <i>B. pertussis</i> pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of <i>B. pertussis</i>, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on <i>B. pertussis</i> physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of <i>B. pertussis</i> life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on <i>B. pertussis</i> pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/84205
url http://sedici.unlp.edu.ar/handle/10915/84205
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1615-9853
info:eu-repo/semantics/altIdentifier/doi/10.1002/pmic.200800218
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
4995-5010
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instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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