Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract

Autores
Cornejo, María Paula; De Francesco, Pablo Nicolás; García Romero, Guadalupe; Portiansky, Enrique Leo; Zigman, Jeffrey M.; Reynaldo, Mirta Beatriz; Perelló, Mario Carlos
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ghrelin is a stomach-derived hormone that regulates a variety of biological functions such as food intake, gastrointestinal function and blood glucose metabolism, among others. Ghrelin acts via the growth hormone secretagogue receptor (GHSR), a G-protein-coupled receptor located in key brain areas that mediate specific actions of the hormone. GHSR is highly expressed in the nucleus of the solitary tract (NTS), which is located in the medulla oblongata and controls essential functions, including orofacial, autonomic, neuroendocrine and behavioral responses. Here, we used a mouse model, in which the expression of enhanced green fluorescent protein (eGFP) is controlled by the promoter of GHSR (GHSR-eGFP mice), to gain neuroanatomical and functional insights of the GHSR-expressing neurons of the NTS. We found that GHSR-expressing neurons of the NTS are segregated in clusters that were symmetrically distributed to the midline: (1) a pair of rostral clusters, and (2) a caudal and medially located cluster. We also identified that a subset of GHSR neurons of the caudal NTS are GABAergic. Finally, we found that rostral NTS GHSR neurons increase the levels of the marker of neuronal activation c-Fos in mice exposed to fasting/refeeding or high-fat diet bingeing protocols, while caudal NTS GHSR neurons increase the levels of c-Fos in mice exposed to gastric distension or LiCl-induced malaise protocols. Thus, current data provide evidence that ghrelin receptor signaling seems to target segregated clusters of neurons within the NTS that, in turn, may be activated by different stimuli.
Instituto Multidisciplinario de Biología Celular
Facultad de Ciencias Veterinarias
Materia
Biología
Dorsal vagal complex
Medulla oblongata
GABA neurons
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137654
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/137654
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tractCornejo, María PaulaDe Francesco, Pablo NicolásGarcía Romero, GuadalupePortiansky, Enrique LeoZigman, Jeffrey M.Reynaldo, Mirta BeatrizPerelló, Mario CarlosBiologíaDorsal vagal complexMedulla oblongataGABA neuronsGhrelin is a stomach-derived hormone that regulates a variety of biological functions such as food intake, gastrointestinal function and blood glucose metabolism, among others. Ghrelin acts via the growth hormone secretagogue receptor (GHSR), a G-protein-coupled receptor located in key brain areas that mediate specific actions of the hormone. GHSR is highly expressed in the nucleus of the solitary tract (NTS), which is located in the medulla oblongata and controls essential functions, including orofacial, autonomic, neuroendocrine and behavioral responses. Here, we used a mouse model, in which the expression of enhanced green fluorescent protein (eGFP) is controlled by the promoter of GHSR (GHSR-eGFP mice), to gain neuroanatomical and functional insights of the GHSR-expressing neurons of the NTS. We found that GHSR-expressing neurons of the NTS are segregated in clusters that were symmetrically distributed to the midline: (1) a pair of rostral clusters, and (2) a caudal and medially located cluster. We also identified that a subset of GHSR neurons of the caudal NTS are GABAergic. Finally, we found that rostral NTS GHSR neurons increase the levels of the marker of neuronal activation c-Fos in mice exposed to fasting/refeeding or high-fat diet bingeing protocols, while caudal NTS GHSR neurons increase the levels of c-Fos in mice exposed to gastric distension or LiCl-induced malaise protocols. Thus, current data provide evidence that ghrelin receptor signaling seems to target segregated clusters of neurons within the NTS that, in turn, may be activated by different stimuli.Instituto Multidisciplinario de Biología CelularFacultad de Ciencias Veterinarias2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf3133-3147http://sedici.unlp.edu.ar/handle/10915/137654enginfo:eu-repo/semantics/altIdentifier/issn/1863-2661info:eu-repo/semantics/altIdentifier/issn/1863-2653info:eu-repo/semantics/altIdentifier/doi/10.1007/s00429-018-1682-5info:eu-repo/semantics/altIdentifier/pmid/29761230info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:14:45Zoai:sedici.unlp.edu.ar:10915/137654Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:14:45.941SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
title Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
spellingShingle Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
Cornejo, María Paula
Biología
Dorsal vagal complex
Medulla oblongata
GABA neurons
title_short Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
title_full Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
title_fullStr Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
title_full_unstemmed Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
title_sort Ghrelin receptor signaling targets segregated clusters of neurons within the nucleus of the solitary tract
dc.creator.none.fl_str_mv Cornejo, María Paula
De Francesco, Pablo Nicolás
García Romero, Guadalupe
Portiansky, Enrique Leo
Zigman, Jeffrey M.
Reynaldo, Mirta Beatriz
Perelló, Mario Carlos
author Cornejo, María Paula
author_facet Cornejo, María Paula
De Francesco, Pablo Nicolás
García Romero, Guadalupe
Portiansky, Enrique Leo
Zigman, Jeffrey M.
Reynaldo, Mirta Beatriz
Perelló, Mario Carlos
author_role author
author2 De Francesco, Pablo Nicolás
García Romero, Guadalupe
Portiansky, Enrique Leo
Zigman, Jeffrey M.
Reynaldo, Mirta Beatriz
Perelló, Mario Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Dorsal vagal complex
Medulla oblongata
GABA neurons
topic Biología
Dorsal vagal complex
Medulla oblongata
GABA neurons
dc.description.none.fl_txt_mv Ghrelin is a stomach-derived hormone that regulates a variety of biological functions such as food intake, gastrointestinal function and blood glucose metabolism, among others. Ghrelin acts via the growth hormone secretagogue receptor (GHSR), a G-protein-coupled receptor located in key brain areas that mediate specific actions of the hormone. GHSR is highly expressed in the nucleus of the solitary tract (NTS), which is located in the medulla oblongata and controls essential functions, including orofacial, autonomic, neuroendocrine and behavioral responses. Here, we used a mouse model, in which the expression of enhanced green fluorescent protein (eGFP) is controlled by the promoter of GHSR (GHSR-eGFP mice), to gain neuroanatomical and functional insights of the GHSR-expressing neurons of the NTS. We found that GHSR-expressing neurons of the NTS are segregated in clusters that were symmetrically distributed to the midline: (1) a pair of rostral clusters, and (2) a caudal and medially located cluster. We also identified that a subset of GHSR neurons of the caudal NTS are GABAergic. Finally, we found that rostral NTS GHSR neurons increase the levels of the marker of neuronal activation c-Fos in mice exposed to fasting/refeeding or high-fat diet bingeing protocols, while caudal NTS GHSR neurons increase the levels of c-Fos in mice exposed to gastric distension or LiCl-induced malaise protocols. Thus, current data provide evidence that ghrelin receptor signaling seems to target segregated clusters of neurons within the NTS that, in turn, may be activated by different stimuli.
Instituto Multidisciplinario de Biología Celular
Facultad de Ciencias Veterinarias
description Ghrelin is a stomach-derived hormone that regulates a variety of biological functions such as food intake, gastrointestinal function and blood glucose metabolism, among others. Ghrelin acts via the growth hormone secretagogue receptor (GHSR), a G-protein-coupled receptor located in key brain areas that mediate specific actions of the hormone. GHSR is highly expressed in the nucleus of the solitary tract (NTS), which is located in the medulla oblongata and controls essential functions, including orofacial, autonomic, neuroendocrine and behavioral responses. Here, we used a mouse model, in which the expression of enhanced green fluorescent protein (eGFP) is controlled by the promoter of GHSR (GHSR-eGFP mice), to gain neuroanatomical and functional insights of the GHSR-expressing neurons of the NTS. We found that GHSR-expressing neurons of the NTS are segregated in clusters that were symmetrically distributed to the midline: (1) a pair of rostral clusters, and (2) a caudal and medially located cluster. We also identified that a subset of GHSR neurons of the caudal NTS are GABAergic. Finally, we found that rostral NTS GHSR neurons increase the levels of the marker of neuronal activation c-Fos in mice exposed to fasting/refeeding or high-fat diet bingeing protocols, while caudal NTS GHSR neurons increase the levels of c-Fos in mice exposed to gastric distension or LiCl-induced malaise protocols. Thus, current data provide evidence that ghrelin receptor signaling seems to target segregated clusters of neurons within the NTS that, in turn, may be activated by different stimuli.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137654
url http://sedici.unlp.edu.ar/handle/10915/137654
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1863-2661
info:eu-repo/semantics/altIdentifier/issn/1863-2653
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00429-018-1682-5
info:eu-repo/semantics/altIdentifier/pmid/29761230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
3133-3147
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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