Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus

Autores
Cabral, Agustina Soledad; Portiansky, Enrique Leo; Sánchez Jaramillo, Edith; Zigman, Jeffrey M.; Perello, Mario
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin's orexigenic action vs. its role as a stress signal are anatomically dissociated.
Fil: Cabral, Agustina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez Jaramillo, Edith. Instituto Nacional de Psiquiatria Ramon de la Fuente; México
Fil: Zigman, Jeffrey M.. University of Texas. Southwestern Medical Center; Estados Unidos
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Materia
GHRELIN
NEUROPEPTIDE Y (NPY)
OREXIGENIC
STRESS
Γ-AMINOBUTYRIC ACID (GABA)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54146
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54146
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleusCabral, Agustina SoledadPortiansky, Enrique LeoSánchez Jaramillo, EdithZigman, Jeffrey M.Perello, MarioGHRELINNEUROPEPTIDE Y (NPY)OREXIGENICSTRESSΓ-AMINOBUTYRIC ACID (GABA)https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin's orexigenic action vs. its role as a stress signal are anatomically dissociated.Fil: Cabral, Agustina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez Jaramillo, Edith. Instituto Nacional de Psiquiatria Ramon de la Fuente; MéxicoFil: Zigman, Jeffrey M.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaPergamon-Elsevier Science Ltd2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54146Cabral, Agustina Soledad; Portiansky, Enrique Leo; Sánchez Jaramillo, Edith; Zigman, Jeffrey M.; Perello, Mario; Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus; Pergamon-Elsevier Science Ltd; Psychoneuroendocrinology; 67; 5-2016; 27-390306-4530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.psyneuen.2016.01.027info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306453016300269info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/26874559/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:42Zoai:ri.conicet.gov.ar:11336/54146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:42.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
title Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
spellingShingle Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
Cabral, Agustina Soledad
GHRELIN
NEUROPEPTIDE Y (NPY)
OREXIGENIC
STRESS
Γ-AMINOBUTYRIC ACID (GABA)
title_short Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
title_full Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
title_fullStr Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
title_full_unstemmed Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
title_sort Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
dc.creator.none.fl_str_mv Cabral, Agustina Soledad
Portiansky, Enrique Leo
Sánchez Jaramillo, Edith
Zigman, Jeffrey M.
Perello, Mario
author Cabral, Agustina Soledad
author_facet Cabral, Agustina Soledad
Portiansky, Enrique Leo
Sánchez Jaramillo, Edith
Zigman, Jeffrey M.
Perello, Mario
author_role author
author2 Portiansky, Enrique Leo
Sánchez Jaramillo, Edith
Zigman, Jeffrey M.
Perello, Mario
author2_role author
author
author
author
dc.subject.none.fl_str_mv GHRELIN
NEUROPEPTIDE Y (NPY)
OREXIGENIC
STRESS
Γ-AMINOBUTYRIC ACID (GABA)
topic GHRELIN
NEUROPEPTIDE Y (NPY)
OREXIGENIC
STRESS
Γ-AMINOBUTYRIC ACID (GABA)
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin's orexigenic action vs. its role as a stress signal are anatomically dissociated.
Fil: Cabral, Agustina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez Jaramillo, Edith. Instituto Nacional de Psiquiatria Ramon de la Fuente; México
Fil: Zigman, Jeffrey M.. University of Texas. Southwestern Medical Center; Estados Unidos
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
description Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin's orexigenic action vs. its role as a stress signal are anatomically dissociated.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54146
Cabral, Agustina Soledad; Portiansky, Enrique Leo; Sánchez Jaramillo, Edith; Zigman, Jeffrey M.; Perello, Mario; Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus; Pergamon-Elsevier Science Ltd; Psychoneuroendocrinology; 67; 5-2016; 27-39
0306-4530
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54146
identifier_str_mv Cabral, Agustina Soledad; Portiansky, Enrique Leo; Sánchez Jaramillo, Edith; Zigman, Jeffrey M.; Perello, Mario; Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus; Pergamon-Elsevier Science Ltd; Psychoneuroendocrinology; 67; 5-2016; 27-39
0306-4530
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.psyneuen.2016.01.027
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306453016300269
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/26874559/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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