Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
- Autores
- Abba, Martín Carlos; Lacunza, Ezequiel; Nunez, María I.; Colussi, Andrea G.; Isla Larrain, Marina Teresita; Segal-Eiras, Amada; Croce, María Virginia; Aldaz, C. Marcelo
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the course of breast cancer global gene expression studies, we identified an uncharacterized gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly over-expressed in primary tumors from patients with recurrent disease. In this study, we identified RHBDD2 mRNA and protein expression significantly elevated in breast carcinomas compared with normal breast samples as analyzed by SAGE (n = 46) and immunohistochemistry (n = 213). Interestingly, specimens displaying RHBDD2 over-expression were predominantly advanced stage III breast carcinomas (p = 0.001). Western-blot, RT-PCR and cDNA sequencing analyses allowed us to identify two RHBDD2 alternatively spliced mRNA isoforms expressed in breast cancer cell lines. We further investigated the occurrence and frequency of gene amplification and over-expression affecting RHBDD2 in 131 breast samples. RHBDD2 gene amplification was detected in 21% of 98 invasive breast carcinomas analyzed. However, no RHBDD2 amplification was detected in normal breast tissues (n = 17) or breast benign lesions (n = 16) (p = 0.014). Interestingly, siRNA-mediated silencing of RHBDD2 expression results in a decrease of MCF7 breast cancer cells proliferation compared with the corresponding controls (p = 0.001). In addition, analysis of publicly available gene expression data showed a strong association between high RHBDD2 expression and decreased overall survival (p = 0.0023), relapse-free survival (p = 0.0013), and metastasis-free interval (p = 0.006) in patients with primary ER-negative breast carcinomas. In conclusion, our findings suggest that RHBDD2 over-expression behaves as an indicator of poor prognosis and may play a role facilitating breast cancer progression.
Facultad de Ciencias Médicas - Materia
-
Ciencias Médicas
Breast cancer
Gene amplification
Gene expression profile
RHBDD2 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/82772
Ver los metadatos del registro completo
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Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancerAbba, Martín CarlosLacunza, EzequielNunez, María I.Colussi, Andrea G.Isla Larrain, Marina TeresitaSegal-Eiras, AmadaCroce, María VirginiaAldaz, C. MarceloCiencias MédicasBreast cancerGene amplificationGene expression profileRHBDD2In the course of breast cancer global gene expression studies, we identified an uncharacterized gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly over-expressed in primary tumors from patients with recurrent disease. In this study, we identified RHBDD2 mRNA and protein expression significantly elevated in breast carcinomas compared with normal breast samples as analyzed by SAGE (n = 46) and immunohistochemistry (n = 213). Interestingly, specimens displaying RHBDD2 over-expression were predominantly advanced stage III breast carcinomas (p = 0.001). Western-blot, RT-PCR and cDNA sequencing analyses allowed us to identify two RHBDD2 alternatively spliced mRNA isoforms expressed in breast cancer cell lines. We further investigated the occurrence and frequency of gene amplification and over-expression affecting RHBDD2 in 131 breast samples. RHBDD2 gene amplification was detected in 21% of 98 invasive breast carcinomas analyzed. However, no RHBDD2 amplification was detected in normal breast tissues (n = 17) or breast benign lesions (n = 16) (p = 0.014). Interestingly, siRNA-mediated silencing of RHBDD2 expression results in a decrease of MCF7 breast cancer cells proliferation compared with the corresponding controls (p = 0.001). In addition, analysis of publicly available gene expression data showed a strong association between high RHBDD2 expression and decreased overall survival (p = 0.0023), relapse-free survival (p = 0.0013), and metastasis-free interval (p = 0.006) in patients with primary ER-negative breast carcinomas. In conclusion, our findings suggest that RHBDD2 over-expression behaves as an indicator of poor prognosis and may play a role facilitating breast cancer progression.Facultad de Ciencias Médicas2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf988-997http://sedici.unlp.edu.ar/handle/10915/82772enginfo:eu-repo/semantics/altIdentifier/issn/0925-4439info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbadis.2009.07.006info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:35Zoai:sedici.unlp.edu.ar:10915/82772Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:36.237SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
title |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
spellingShingle |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer Abba, Martín Carlos Ciencias Médicas Breast cancer Gene amplification Gene expression profile RHBDD2 |
title_short |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
title_full |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
title_fullStr |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
title_full_unstemmed |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
title_sort |
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer |
dc.creator.none.fl_str_mv |
Abba, Martín Carlos Lacunza, Ezequiel Nunez, María I. Colussi, Andrea G. Isla Larrain, Marina Teresita Segal-Eiras, Amada Croce, María Virginia Aldaz, C. Marcelo |
author |
Abba, Martín Carlos |
author_facet |
Abba, Martín Carlos Lacunza, Ezequiel Nunez, María I. Colussi, Andrea G. Isla Larrain, Marina Teresita Segal-Eiras, Amada Croce, María Virginia Aldaz, C. Marcelo |
author_role |
author |
author2 |
Lacunza, Ezequiel Nunez, María I. Colussi, Andrea G. Isla Larrain, Marina Teresita Segal-Eiras, Amada Croce, María Virginia Aldaz, C. Marcelo |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Médicas Breast cancer Gene amplification Gene expression profile RHBDD2 |
topic |
Ciencias Médicas Breast cancer Gene amplification Gene expression profile RHBDD2 |
dc.description.none.fl_txt_mv |
In the course of breast cancer global gene expression studies, we identified an uncharacterized gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly over-expressed in primary tumors from patients with recurrent disease. In this study, we identified RHBDD2 mRNA and protein expression significantly elevated in breast carcinomas compared with normal breast samples as analyzed by SAGE (n = 46) and immunohistochemistry (n = 213). Interestingly, specimens displaying RHBDD2 over-expression were predominantly advanced stage III breast carcinomas (p = 0.001). Western-blot, RT-PCR and cDNA sequencing analyses allowed us to identify two RHBDD2 alternatively spliced mRNA isoforms expressed in breast cancer cell lines. We further investigated the occurrence and frequency of gene amplification and over-expression affecting RHBDD2 in 131 breast samples. RHBDD2 gene amplification was detected in 21% of 98 invasive breast carcinomas analyzed. However, no RHBDD2 amplification was detected in normal breast tissues (n = 17) or breast benign lesions (n = 16) (p = 0.014). Interestingly, siRNA-mediated silencing of RHBDD2 expression results in a decrease of MCF7 breast cancer cells proliferation compared with the corresponding controls (p = 0.001). In addition, analysis of publicly available gene expression data showed a strong association between high RHBDD2 expression and decreased overall survival (p = 0.0023), relapse-free survival (p = 0.0013), and metastasis-free interval (p = 0.006) in patients with primary ER-negative breast carcinomas. In conclusion, our findings suggest that RHBDD2 over-expression behaves as an indicator of poor prognosis and may play a role facilitating breast cancer progression. Facultad de Ciencias Médicas |
description |
In the course of breast cancer global gene expression studies, we identified an uncharacterized gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly over-expressed in primary tumors from patients with recurrent disease. In this study, we identified RHBDD2 mRNA and protein expression significantly elevated in breast carcinomas compared with normal breast samples as analyzed by SAGE (n = 46) and immunohistochemistry (n = 213). Interestingly, specimens displaying RHBDD2 over-expression were predominantly advanced stage III breast carcinomas (p = 0.001). Western-blot, RT-PCR and cDNA sequencing analyses allowed us to identify two RHBDD2 alternatively spliced mRNA isoforms expressed in breast cancer cell lines. We further investigated the occurrence and frequency of gene amplification and over-expression affecting RHBDD2 in 131 breast samples. RHBDD2 gene amplification was detected in 21% of 98 invasive breast carcinomas analyzed. However, no RHBDD2 amplification was detected in normal breast tissues (n = 17) or breast benign lesions (n = 16) (p = 0.014). Interestingly, siRNA-mediated silencing of RHBDD2 expression results in a decrease of MCF7 breast cancer cells proliferation compared with the corresponding controls (p = 0.001). In addition, analysis of publicly available gene expression data showed a strong association between high RHBDD2 expression and decreased overall survival (p = 0.0023), relapse-free survival (p = 0.0013), and metastasis-free interval (p = 0.006) in patients with primary ER-negative breast carcinomas. In conclusion, our findings suggest that RHBDD2 over-expression behaves as an indicator of poor prognosis and may play a role facilitating breast cancer progression. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 |
dc.type.none.fl_str_mv |
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http://sedici.unlp.edu.ar/handle/10915/82772 |
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