RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer

Autores
Lacunza, Ezequiel; Canzoneri, Romina; Rabassa, Martín Enrique; Zwenger, Ariel Osvaldo; Segal-Eiras, Amada; Croce, María Virginia; Abba, Martín Carlos
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA microarray studies were compiled in a homogeneous dataset of 906 colorectal samples. The statistical analysis of these data showed a significant increase of RHBDD2 expression in the advanced stages of CRC (p < 0.01). We validated these findings by immunohistochemistry on 130 colorectal tissue samples; RHBDD2 protein overexpression was also observed in the advanced stages of the disease (p < 0.001). In addition, we investigated RHBDD2 expression in response to the chemotherapy agent 5-fluorouracile (5FU). We detected a significant increase of RHBDD2 mRNA and protein after 5FU treatment (20–40 μM; p < 0.001). Overall, these results showed that RHBDD2 overexpression might play a role in colorectal cancer progression.
Facultad de Ciencias Médicas
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Materia
Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/133492
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/133492
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancerLacunza, EzequielCanzoneri, RominaRabassa, Martín EnriqueZwenger, Ariel OsvaldoSegal-Eiras, AmadaCroce, María VirginiaAbba, Martín CarlosMedicinaColorectal cancerRHBDD2 gene expression5FU treatmentIn previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA microarray studies were compiled in a homogeneous dataset of 906 colorectal samples. The statistical analysis of these data showed a significant increase of RHBDD2 expression in the advanced stages of CRC (p < 0.01). We validated these findings by immunohistochemistry on 130 colorectal tissue samples; RHBDD2 protein overexpression was also observed in the advanced stages of the disease (p < 0.001). In addition, we investigated RHBDD2 expression in response to the chemotherapy agent 5-fluorouracile (5FU). We detected a significant increase of RHBDD2 mRNA and protein after 5FU treatment (20–40 μM; p < 0.001). Overall, these results showed that RHBDD2 overexpression might play a role in colorectal cancer progression.Facultad de Ciencias MédicasCentro de Investigaciones Inmunológicas Básicas y Aplicadas2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2393-2399http://sedici.unlp.edu.ar/handle/10915/133492enginfo:eu-repo/semantics/altIdentifier/issn/1423-0380info:eu-repo/semantics/altIdentifier/issn/1010-4283info:eu-repo/semantics/altIdentifier/doi/10.1007/s13277-012-0503-3info:eu-repo/semantics/altIdentifier/pmid/22965880info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:12:47Zoai:sedici.unlp.edu.ar:10915/133492Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:12:47.614SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
spellingShingle RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
Lacunza, Ezequiel
Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
title_short RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_full RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_fullStr RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_full_unstemmed RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_sort RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
dc.creator.none.fl_str_mv Lacunza, Ezequiel
Canzoneri, Romina
Rabassa, Martín Enrique
Zwenger, Ariel Osvaldo
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author Lacunza, Ezequiel
author_facet Lacunza, Ezequiel
Canzoneri, Romina
Rabassa, Martín Enrique
Zwenger, Ariel Osvaldo
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author_role author
author2 Canzoneri, Romina
Rabassa, Martín Enrique
Zwenger, Ariel Osvaldo
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
topic Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
dc.description.none.fl_txt_mv In previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA microarray studies were compiled in a homogeneous dataset of 906 colorectal samples. The statistical analysis of these data showed a significant increase of RHBDD2 expression in the advanced stages of CRC (p < 0.01). We validated these findings by immunohistochemistry on 130 colorectal tissue samples; RHBDD2 protein overexpression was also observed in the advanced stages of the disease (p < 0.001). In addition, we investigated RHBDD2 expression in response to the chemotherapy agent 5-fluorouracile (5FU). We detected a significant increase of RHBDD2 mRNA and protein after 5FU treatment (20–40 μM; p < 0.001). Overall, these results showed that RHBDD2 overexpression might play a role in colorectal cancer progression.
Facultad de Ciencias Médicas
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
description In previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA microarray studies were compiled in a homogeneous dataset of 906 colorectal samples. The statistical analysis of these data showed a significant increase of RHBDD2 expression in the advanced stages of CRC (p < 0.01). We validated these findings by immunohistochemistry on 130 colorectal tissue samples; RHBDD2 protein overexpression was also observed in the advanced stages of the disease (p < 0.001). In addition, we investigated RHBDD2 expression in response to the chemotherapy agent 5-fluorouracile (5FU). We detected a significant increase of RHBDD2 mRNA and protein after 5FU treatment (20–40 μM; p < 0.001). Overall, these results showed that RHBDD2 overexpression might play a role in colorectal cancer progression.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/133492
url http://sedici.unlp.edu.ar/handle/10915/133492
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1423-0380
info:eu-repo/semantics/altIdentifier/issn/1010-4283
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13277-012-0503-3
info:eu-repo/semantics/altIdentifier/pmid/22965880
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
2393-2399
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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