Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital
- Autores
- Crivaro, Andrea Natalia; Carasi, Paula; Salto, Ileana Paula; Hugo, Ayelén Amelia; Soldavini Pelichotti, Paola Cecilia; Bengoa, Ana Agustina; Fragomeno, Melisa; Serradell, María de los Ángeles; Minnaard, Jessica; Rolny, Ivanna Sabrina; Alul, Eduardo; Arregui, Leandro; Fabra Martinez, Macarena E.; Moreno Valero, Oscar Javier; Facente, Andrea; Magariños, Francisco; Jewtuchowicz, Virginia; Pérez, Pablo Fernando; Trejo, Fernando Miguel
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik’s classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Estudios Inmunológicos y Fisiopatológicos
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
Clostridioides difficile
Toxinotyping
RFLP
Virulence
Antibiotic-associated diarrhea - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/159934
Ver los metadatos del registro completo
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Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospitalCrivaro, Andrea NataliaCarasi, PaulaSalto, Ileana PaulaHugo, Ayelén AmeliaSoldavini Pelichotti, Paola CeciliaBengoa, Ana AgustinaFragomeno, MelisaSerradell, María de los ÁngelesMinnaard, JessicaRolny, Ivanna SabrinaAlul, EduardoArregui, LeandroFabra Martinez, Macarena E.Moreno Valero, Oscar JavierFacente, AndreaMagariños, FranciscoJewtuchowicz, VirginiaPérez, Pablo FernandoTrejo, Fernando MiguelBiologíaClostridioides difficileToxinotypingRFLPVirulenceAntibiotic-associated diarrheaClostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik’s classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins.Centro de Investigación y Desarrollo en Criotecnología de AlimentosInstituto de Estudios Inmunológicos y FisiopatológicosInstituto de Biotecnología y Biología Molecular2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf73-82http://sedici.unlp.edu.ar/handle/10915/159934enginfo:eu-repo/semantics/altIdentifier/issn/0325-7541info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2022.05.010info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-26T10:19:48Zoai:sedici.unlp.edu.ar:10915/159934Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-26 10:19:48.479SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| spellingShingle |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital Crivaro, Andrea Natalia Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea |
| title_short |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_full |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_fullStr |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_full_unstemmed |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_sort |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| dc.creator.none.fl_str_mv |
Crivaro, Andrea Natalia Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel |
| author |
Crivaro, Andrea Natalia |
| author_facet |
Crivaro, Andrea Natalia Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel |
| author_role |
author |
| author2 |
Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea |
| topic |
Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea |
| dc.description.none.fl_txt_mv |
Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik’s classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Instituto de Estudios Inmunológicos y Fisiopatológicos Instituto de Biotecnología y Biología Molecular |
| description |
Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik’s classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins. |
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2023 |
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2023 |
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