Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119

Autores
Ludueña, Liliana Mercedes; Anzuay, María Soledad; Angelini, Jorge Guillermo; Barros, Germán; Luna, María Flavia; Monge, María del Pilar; Fabra, Adriana; Taurian, Tania
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to analyze if cofactor pyrroquinoline quinone from Serratia sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain Serratia sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant Serratia sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within Serratia strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from Serratia sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of Serratia sp. S119 but is not required for growth promotion of peanut and maize plants.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/132692
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/132692
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119Ludueña, Liliana MercedesAnzuay, María SoledadAngelini, Jorge GuillermoBarros, GermánLuna, María FlaviaMonge, María del PilarFabra, AdrianaTaurian, TaniaCiencias ExactasQuímicaPQQ cofactorSerratia sp.Phosphate solubilizationPlant growth promotionpqqE sequenceThe aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain <i>Serratia</i> sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant <i>Serratia</i> sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within <i>Serratia</i> strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from <i>Serratia</i> sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of <i>Serratia</i> sp. S119 but is not required for growth promotion of peanut and maize plants.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf31-43http://sedici.unlp.edu.ar/handle/10915/132692enginfo:eu-repo/semantics/altIdentifier/issn/0334-5114info:eu-repo/semantics/altIdentifier/issn/1878-7665info:eu-repo/semantics/altIdentifier/doi/10.1007/s13199-016-0434-7info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-05T13:11:21Zoai:sedici.unlp.edu.ar:10915/132692Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-05 13:11:22.312SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
spellingShingle Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
Ludueña, Liliana Mercedes
Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
title_short Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_full Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_fullStr Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_full_unstemmed Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_sort Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
dc.creator.none.fl_str_mv Ludueña, Liliana Mercedes
Anzuay, María Soledad
Angelini, Jorge Guillermo
Barros, Germán
Luna, María Flavia
Monge, María del Pilar
Fabra, Adriana
Taurian, Tania
author Ludueña, Liliana Mercedes
author_facet Ludueña, Liliana Mercedes
Anzuay, María Soledad
Angelini, Jorge Guillermo
Barros, Germán
Luna, María Flavia
Monge, María del Pilar
Fabra, Adriana
Taurian, Tania
author_role author
author2 Anzuay, María Soledad
Angelini, Jorge Guillermo
Barros, Germán
Luna, María Flavia
Monge, María del Pilar
Fabra, Adriana
Taurian, Tania
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
topic Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
dc.description.none.fl_txt_mv The aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain <i>Serratia</i> sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant <i>Serratia</i> sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within <i>Serratia</i> strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from <i>Serratia</i> sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of <i>Serratia</i> sp. S119 but is not required for growth promotion of peanut and maize plants.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description The aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain <i>Serratia</i> sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant <i>Serratia</i> sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within <i>Serratia</i> strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from <i>Serratia</i> sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of <i>Serratia</i> sp. S119 but is not required for growth promotion of peanut and maize plants.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/132692
url http://sedici.unlp.edu.ar/handle/10915/132692
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0334-5114
info:eu-repo/semantics/altIdentifier/issn/1878-7665
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13199-016-0434-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
31-43
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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