Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants

Autores
Anzuay, María Soledad; Chiatti, Mario Hernán; Intelangelo, Ariana Belén; Ludueña, Liliana Mercedes; Pin Viso, Natalia Daniela; Angelini, Jorge Guillermo; Taurian, Tania
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populations
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Anzuay, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Chiatti, Mario Hernán. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Chiatti, Mario Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Intelangelo, Ariana Belén. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Intelangelo, Ariana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Ludueña, Liliana Mercedes. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Ludueña, Liliana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Angelini, Jorge Guillermo. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Angelini, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Taurian, Tania. Universidad Nacional de Río Cuarto (UNRC). Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fuente
Current Genetics 70 : article number: 12 (August 2024)
Materia
Arachis hypogaea
Marcadores Genéticos
Factor Abiótico
Factor Biótico
Filogenia
Bacterias del Suelo
Bacteria Gram Negativa
Genetic Markers
Abiotic Factors
Biotic Factors
Phylogeny
Soil Bacteria
Gram-negative Bacteria
Pyrroloquinoline Quinone Biogenesis
Phosphate Solubilizing Bacteria
pqqE Gene
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21125
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network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plantsAnzuay, María SoledadChiatti, Mario HernánIntelangelo, Ariana BelénLudueña, Liliana MercedesPin Viso, Natalia DanielaAngelini, Jorge GuillermoTaurian, TaniaArachis hypogaeaMarcadores GenéticosFactor AbióticoFactor BióticoFilogeniaBacterias del SueloBacteria Gram NegativaGenetic MarkersAbiotic FactorsBiotic FactorsPhylogenySoil BacteriaGram-negative BacteriaPyrroloquinoline Quinone BiogenesisPhosphate Solubilizing BacteriapqqE GeneInsoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populationsInstituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Anzuay, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Chiatti, Mario Hernán. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Chiatti, Mario Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Intelangelo, Ariana Belén. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Intelangelo, Ariana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Ludueña, Liliana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Angelini, Jorge Guillermo. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Angelini, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Taurian, Tania. Universidad Nacional de Río Cuarto (UNRC). Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; ArgentinaSpringer2025-02-04T14:10:29Z2025-02-04T14:10:29Z2024-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21125https://link.springer.com/article/10.1007/s00294-024-01296-40172-8083 (impreso)1432-0983 (online)https://doi.org/10.1007/s00294-024-01296-4Current Genetics 70 : article number: 12 (August 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:53Zoai:localhost:20.500.12123/21125instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:53.578INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
title Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
spellingShingle Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
Anzuay, María Soledad
Arachis hypogaea
Marcadores Genéticos
Factor Abiótico
Factor Biótico
Filogenia
Bacterias del Suelo
Bacteria Gram Negativa
Genetic Markers
Abiotic Factors
Biotic Factors
Phylogeny
Soil Bacteria
Gram-negative Bacteria
Pyrroloquinoline Quinone Biogenesis
Phosphate Solubilizing Bacteria
pqqE Gene
title_short Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
title_full Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
title_fullStr Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
title_full_unstemmed Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
title_sort Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
dc.creator.none.fl_str_mv Anzuay, María Soledad
Chiatti, Mario Hernán
Intelangelo, Ariana Belén
Ludueña, Liliana Mercedes
Pin Viso, Natalia Daniela
Angelini, Jorge Guillermo
Taurian, Tania
author Anzuay, María Soledad
author_facet Anzuay, María Soledad
Chiatti, Mario Hernán
Intelangelo, Ariana Belén
Ludueña, Liliana Mercedes
Pin Viso, Natalia Daniela
Angelini, Jorge Guillermo
Taurian, Tania
author_role author
author2 Chiatti, Mario Hernán
Intelangelo, Ariana Belén
Ludueña, Liliana Mercedes
Pin Viso, Natalia Daniela
Angelini, Jorge Guillermo
Taurian, Tania
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Arachis hypogaea
Marcadores Genéticos
Factor Abiótico
Factor Biótico
Filogenia
Bacterias del Suelo
Bacteria Gram Negativa
Genetic Markers
Abiotic Factors
Biotic Factors
Phylogeny
Soil Bacteria
Gram-negative Bacteria
Pyrroloquinoline Quinone Biogenesis
Phosphate Solubilizing Bacteria
pqqE Gene
topic Arachis hypogaea
Marcadores Genéticos
Factor Abiótico
Factor Biótico
Filogenia
Bacterias del Suelo
Bacteria Gram Negativa
Genetic Markers
Abiotic Factors
Biotic Factors
Phylogeny
Soil Bacteria
Gram-negative Bacteria
Pyrroloquinoline Quinone Biogenesis
Phosphate Solubilizing Bacteria
pqqE Gene
dc.description.none.fl_txt_mv Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populations
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Anzuay, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Chiatti, Mario Hernán. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Chiatti, Mario Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Intelangelo, Ariana Belén. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Intelangelo, Ariana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Ludueña, Liliana Mercedes. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Ludueña, Liliana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Angelini, Jorge Guillermo. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Angelini, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Taurian, Tania. Universidad Nacional de Río Cuarto (UNRC). Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina
description Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populations
publishDate 2024
dc.date.none.fl_str_mv 2024-08-02
2025-02-04T14:10:29Z
2025-02-04T14:10:29Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21125
https://link.springer.com/article/10.1007/s00294-024-01296-4
0172-8083 (impreso)
1432-0983 (online)
https://doi.org/10.1007/s00294-024-01296-4
url http://hdl.handle.net/20.500.12123/21125
https://link.springer.com/article/10.1007/s00294-024-01296-4
https://doi.org/10.1007/s00294-024-01296-4
identifier_str_mv 0172-8083 (impreso)
1432-0983 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Current Genetics 70 : article number: 12 (August 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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