The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
- Autores
- Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverría, María Gabriela; Pecoraro, Marcelo Ricardo Ítalo
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
Facultad de Ciencias Veterinarias - Materia
-
Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/139073
Ver los metadatos del registro completo
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The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastorisTizzano, Marco AntonioSguazza, Guillermo HernánPicotto, Leandro DanielEcheverría, María GabrielaPecoraro, Marcelo Ricardo ÍtaloVeterinariaCanine distemper virusGlycoprotein FPichia pastorisCanine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.Facultad de Ciencias Veterinarias2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/139073enginfo:eu-repo/semantics/altIdentifier/issn/1096-1208info:eu-repo/semantics/altIdentifier/issn/0882-4010info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2020.104094info:eu-repo/semantics/altIdentifier/pmid/32092361info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:03:59Zoai:sedici.unlp.edu.ar:10915/139073Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:03:59.565SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
title |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
spellingShingle |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris Tizzano, Marco Antonio Veterinaria Canine distemper virus Glycoprotein F Pichia pastoris |
title_short |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
title_full |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
title_fullStr |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
title_full_unstemmed |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
title_sort |
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris |
dc.creator.none.fl_str_mv |
Tizzano, Marco Antonio Sguazza, Guillermo Hernán Picotto, Leandro Daniel Echeverría, María Gabriela Pecoraro, Marcelo Ricardo Ítalo |
author |
Tizzano, Marco Antonio |
author_facet |
Tizzano, Marco Antonio Sguazza, Guillermo Hernán Picotto, Leandro Daniel Echeverría, María Gabriela Pecoraro, Marcelo Ricardo Ítalo |
author_role |
author |
author2 |
Sguazza, Guillermo Hernán Picotto, Leandro Daniel Echeverría, María Gabriela Pecoraro, Marcelo Ricardo Ítalo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Veterinaria Canine distemper virus Glycoprotein F Pichia pastoris |
topic |
Veterinaria Canine distemper virus Glycoprotein F Pichia pastoris |
dc.description.none.fl_txt_mv |
Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine. Facultad de Ciencias Veterinarias |
description |
Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/139073 |
url |
http://sedici.unlp.edu.ar/handle/10915/139073 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1096-1208 info:eu-repo/semantics/altIdentifier/issn/0882-4010 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2020.104094 info:eu-repo/semantics/altIdentifier/pmid/32092361 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf |
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Universidad Nacional de La Plata |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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alira@sedici.unlp.edu.ar |
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