The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris

Autores
Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverría, María Gabriela; Pecoraro, Marcelo Ricardo Ítalo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
Facultad de Ciencias Veterinarias
Materia
Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/139073
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/139073
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastorisTizzano, Marco AntonioSguazza, Guillermo HernánPicotto, Leandro DanielEcheverría, María GabrielaPecoraro, Marcelo Ricardo ÍtaloVeterinariaCanine distemper virusGlycoprotein FPichia pastorisCanine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.Facultad de Ciencias Veterinarias2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/139073enginfo:eu-repo/semantics/altIdentifier/issn/1096-1208info:eu-repo/semantics/altIdentifier/issn/0882-4010info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2020.104094info:eu-repo/semantics/altIdentifier/pmid/32092361info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:53Zoai:sedici.unlp.edu.ar:10915/139073Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:53.341SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
spellingShingle The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
Tizzano, Marco Antonio
Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
title_short The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_full The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_fullStr The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_full_unstemmed The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_sort The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
dc.creator.none.fl_str_mv Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
author Tizzano, Marco Antonio
author_facet Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
author_role author
author2 Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
topic Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
dc.description.none.fl_txt_mv Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
Facultad de Ciencias Veterinarias
description Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/139073
url http://sedici.unlp.edu.ar/handle/10915/139073
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1096-1208
info:eu-repo/semantics/altIdentifier/issn/0882-4010
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2020.104094
info:eu-repo/semantics/altIdentifier/pmid/32092361
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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