Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii
- Autores
- Cavalitto, Sebastián Fernando; Hours, Roque Alberto; Mignone, Carlos Fernando
- Año de publicación
- 1997
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A method for quantification of a pectin releasing enzyme (PPase-SE) from Geotrichum klebahnii (= Geotrichum penicillatum = Trichosporon penicillatum) ATCC 42397 is reported. PPase activity was determined by measuring the amount of soluble pectin released from lemon protopectin. Particle size of the substrate, reaction time and linearity range of the assay, were analysed. The best assay conditions were a reaction time of 30 min, 20 mg substrate (mesh 60) and up to 0.045 units PPase activity per test tube.
Centro de Investigación y Desarrollo en Fermentaciones Industriales - Materia
-
Biología
Bioquímica
Enzyme
Particle Size
Reaction Time
Test Tube
Pectin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/144032
Ver los metadatos del registro completo
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Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahniiCavalitto, Sebastián FernandoHours, Roque AlbertoMignone, Carlos FernandoBiologíaBioquímicaEnzymeParticle SizeReaction TimeTest TubePectinA method for quantification of a pectin releasing enzyme (PPase-SE) from Geotrichum klebahnii (= Geotrichum penicillatum = Trichosporon penicillatum) ATCC 42397 is reported. PPase activity was determined by measuring the amount of soluble pectin released from lemon protopectin. Particle size of the substrate, reaction time and linearity range of the assay, were analysed. The best assay conditions were a reaction time of 30 min, 20 mg substrate (mesh 60) and up to 0.045 units PPase activity per test tube.Centro de Investigación y Desarrollo en Fermentaciones Industriales1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf331-334http://sedici.unlp.edu.ar/handle/10915/144032enginfo:eu-repo/semantics/altIdentifier/issn/0951-208Xinfo:eu-repo/semantics/altIdentifier/issn/1573-6784info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1018427614046info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:38Zoai:sedici.unlp.edu.ar:10915/144032Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:38.244SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| title |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| spellingShingle |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii Cavalitto, Sebastián Fernando Biología Bioquímica Enzyme Particle Size Reaction Time Test Tube Pectin |
| title_short |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| title_full |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| title_fullStr |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| title_full_unstemmed |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| title_sort |
Quantification of pectin-releasing activity of protopectinase-SE from Geotrichum klebahnii |
| dc.creator.none.fl_str_mv |
Cavalitto, Sebastián Fernando Hours, Roque Alberto Mignone, Carlos Fernando |
| author |
Cavalitto, Sebastián Fernando |
| author_facet |
Cavalitto, Sebastián Fernando Hours, Roque Alberto Mignone, Carlos Fernando |
| author_role |
author |
| author2 |
Hours, Roque Alberto Mignone, Carlos Fernando |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Biología Bioquímica Enzyme Particle Size Reaction Time Test Tube Pectin |
| topic |
Biología Bioquímica Enzyme Particle Size Reaction Time Test Tube Pectin |
| dc.description.none.fl_txt_mv |
A method for quantification of a pectin releasing enzyme (PPase-SE) from Geotrichum klebahnii (= Geotrichum penicillatum = Trichosporon penicillatum) ATCC 42397 is reported. PPase activity was determined by measuring the amount of soluble pectin released from lemon protopectin. Particle size of the substrate, reaction time and linearity range of the assay, were analysed. The best assay conditions were a reaction time of 30 min, 20 mg substrate (mesh 60) and up to 0.045 units PPase activity per test tube. Centro de Investigación y Desarrollo en Fermentaciones Industriales |
| description |
A method for quantification of a pectin releasing enzyme (PPase-SE) from Geotrichum klebahnii (= Geotrichum penicillatum = Trichosporon penicillatum) ATCC 42397 is reported. PPase activity was determined by measuring the amount of soluble pectin released from lemon protopectin. Particle size of the substrate, reaction time and linearity range of the assay, were analysed. The best assay conditions were a reaction time of 30 min, 20 mg substrate (mesh 60) and up to 0.045 units PPase activity per test tube. |
| publishDate |
1997 |
| dc.date.none.fl_str_mv |
1997 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/144032 |
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http://sedici.unlp.edu.ar/handle/10915/144032 |
| dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/0951-208X info:eu-repo/semantics/altIdentifier/issn/1573-6784 info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1018427614046 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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