Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of...

Autores
Cunningham, Mónica Liliana; Pollero, Ricardo José; González, Alda
Año de publicación
1994
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract A high-density lipoprotein (HDL) fraction was isolated from the hemolymphatic plasma of the spider Polybetes pythagoricus by density-gradient ultracentrifugation. Hydrated density (1.13 g/ml), electrophoretic mobility (SDS—PAGE) of apoproteins and lipid classes composition were determined. Lipids were identified by HP-TLC and auxiliary techniques; they were quantified by TLC-FID. The protein moiety is composed of two main apoproteins (250 and 76 kDa, respectively) and several polypeptides of low molecular weight. It resembles the apoliphorins of insects and some other arachnids. The lipid composition differs from most lipophorins. Phospholipids amount to more than 60% of total lipids, while diacylglycerols (2.4%) are supplanted by triacylglycerols (16.5%) as the main circulating energetic lipids.
Facultad de Ciencias Naturales y Museo
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Naturales
Biología
Lipids
Transport
Spiders
Hemolymph
Phospholipids
Triacylglycerols
Fatty acids
Arachnida
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/122711

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/122711
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>Cunningham, Mónica LilianaPollero, Ricardo JoséGonzález, AldaCiencias NaturalesBiologíaLipidsTransportSpidersHemolymphPhospholipidsTriacylglycerolsFatty acidsArachnidaAbstract A high-density lipoprotein (HDL) fraction was isolated from the hemolymphatic plasma of the spider <i>Polybetes pythagoricus</i> by density-gradient ultracentrifugation. Hydrated density (1.13 g/ml), electrophoretic mobility (SDS—PAGE) of apoproteins and lipid classes composition were determined. Lipids were identified by HP-TLC and auxiliary techniques; they were quantified by TLC-FID. The protein moiety is composed of two main apoproteins (250 and 76 kDa, respectively) and several polypeptides of low molecular weight. It resembles the apoliphorins of insects and some other arachnids. The lipid composition differs from most lipophorins. Phospholipids amount to more than 60% of total lipids, while diacylglycerols (2.4%) are supplanted by triacylglycerols (16.5%) as the main circulating energetic lipids.Facultad de Ciencias Naturales y MuseoInstituto de Investigaciones Bioquímicas de La Plata1994info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf333-338http://sedici.unlp.edu.ar/handle/10915/122711enginfo:eu-repo/semantics/altIdentifier/issn/0305-0491info:eu-repo/semantics/altIdentifier/doi/10.1016/0305-0491(94)90017-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:14Zoai:sedici.unlp.edu.ar:10915/122711Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:15.053SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
title Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
spellingShingle Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
Cunningham, Mónica Liliana
Ciencias Naturales
Biología
Lipids
Transport
Spiders
Hemolymph
Phospholipids
Triacylglycerols
Fatty acids
Arachnida
title_short Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
title_full Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
title_fullStr Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
title_full_unstemmed Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
title_sort Lipid circulation in spiders : Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of <i>Polybetes pythagoricus</i>
dc.creator.none.fl_str_mv Cunningham, Mónica Liliana
Pollero, Ricardo José
González, Alda
author Cunningham, Mónica Liliana
author_facet Cunningham, Mónica Liliana
Pollero, Ricardo José
González, Alda
author_role author
author2 Pollero, Ricardo José
González, Alda
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Biología
Lipids
Transport
Spiders
Hemolymph
Phospholipids
Triacylglycerols
Fatty acids
Arachnida
topic Ciencias Naturales
Biología
Lipids
Transport
Spiders
Hemolymph
Phospholipids
Triacylglycerols
Fatty acids
Arachnida
dc.description.none.fl_txt_mv Abstract A high-density lipoprotein (HDL) fraction was isolated from the hemolymphatic plasma of the spider <i>Polybetes pythagoricus</i> by density-gradient ultracentrifugation. Hydrated density (1.13 g/ml), electrophoretic mobility (SDS—PAGE) of apoproteins and lipid classes composition were determined. Lipids were identified by HP-TLC and auxiliary techniques; they were quantified by TLC-FID. The protein moiety is composed of two main apoproteins (250 and 76 kDa, respectively) and several polypeptides of low molecular weight. It resembles the apoliphorins of insects and some other arachnids. The lipid composition differs from most lipophorins. Phospholipids amount to more than 60% of total lipids, while diacylglycerols (2.4%) are supplanted by triacylglycerols (16.5%) as the main circulating energetic lipids.
Facultad de Ciencias Naturales y Museo
Instituto de Investigaciones Bioquímicas de La Plata
description Abstract A high-density lipoprotein (HDL) fraction was isolated from the hemolymphatic plasma of the spider <i>Polybetes pythagoricus</i> by density-gradient ultracentrifugation. Hydrated density (1.13 g/ml), electrophoretic mobility (SDS—PAGE) of apoproteins and lipid classes composition were determined. Lipids were identified by HP-TLC and auxiliary techniques; they were quantified by TLC-FID. The protein moiety is composed of two main apoproteins (250 and 76 kDa, respectively) and several polypeptides of low molecular weight. It resembles the apoliphorins of insects and some other arachnids. The lipid composition differs from most lipophorins. Phospholipids amount to more than 60% of total lipids, while diacylglycerols (2.4%) are supplanted by triacylglycerols (16.5%) as the main circulating energetic lipids.
publishDate 1994
dc.date.none.fl_str_mv 1994
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/122711
url http://sedici.unlp.edu.ar/handle/10915/122711
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0305-0491
info:eu-repo/semantics/altIdentifier/doi/10.1016/0305-0491(94)90017-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
333-338
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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