Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis

Autores
Hurtado de Catalfo, Graciela E.; Tacconi de Alaniz, María Josefa; Marra, Carlos Alberto
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to examine the influence of dietary fat on lipid composition, as well as on the steroidogenic function of interstitial cells isolated from Wistar rats that had been fed semi-synthetic diets containing four different commercial oils (S soybean, O olive, C coconut, and G grape seed). Steroidogenic enzyme activities, lipid composition, and androgen production were measured in testicular interstitial cells. Lipid analysis included measurement of the contents of major lipid subclasses (neutral lipids, polar lipids, free and esterified cholesterol), as well as principal polar and neutral lipid fatty acyl compositions. Significant differences in lipid composition were observed among the groups, most of them reflecting the specific fatty acyl composition of the diet tested. Testosterone concentration was higher in O and C groups compared with S or G. In agreement with this observation, the activity of both key enzymes involved in testosterone biosynthesis (3-β-HSD and 17-β-HSD) was higher in O and C groups with significant differences between them (O > C). A significant negative correlation was found between cellular testosterone production and cellular cholesterol ester content. Additionally, testosterone concentration directly correlated with cholesterol levels. We conclude that dietary oils qualitatively and quantitatively modified the lipid composition of interstitial cells, producing either a direct or indirect regulatory effect on testicular steroidogenic function.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Química
Medicina
Lipid composition
Fatty acids
Testosterone production
Testicular interstitial cells
Dietary lipids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140773
Acceder
id SEDICI_7dcac5bfdb197deb676b21493b525466
oai_identifier_str oai:sedici.unlp.edu.ar:10915/140773
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testisHurtado de Catalfo, Graciela E.Tacconi de Alaniz, María JosefaMarra, Carlos AlbertoQuímicaMedicinaLipid compositionFatty acidsTestosterone productionTesticular interstitial cellsDietary lipidsThe aim of this study was to examine the influence of dietary fat on lipid composition, as well as on the steroidogenic function of interstitial cells isolated from Wistar rats that had been fed semi-synthetic diets containing four different commercial oils (S soybean, O olive, C coconut, and G grape seed). Steroidogenic enzyme activities, lipid composition, and androgen production were measured in testicular interstitial cells. Lipid analysis included measurement of the contents of major lipid subclasses (neutral lipids, polar lipids, free and esterified cholesterol), as well as principal polar and neutral lipid fatty acyl compositions. Significant differences in lipid composition were observed among the groups, most of them reflecting the specific fatty acyl composition of the diet tested. Testosterone concentration was higher in O and C groups compared with S or G. In agreement with this observation, the activity of both key enzymes involved in testosterone biosynthesis (3-β-HSD and 17-β-HSD) was higher in O and C groups with significant differences between them (O > C). A significant negative correlation was found between cellular testosterone production and cellular cholesterol ester content. Additionally, testosterone concentration directly correlated with cholesterol levels. We conclude that dietary oils qualitatively and quantitatively modified the lipid composition of interstitial cells, producing either a direct or indirect regulatory effect on testicular steroidogenic function.Instituto de Investigaciones Bioquímicas de La Plata2009-01-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf345-357http://sedici.unlp.edu.ar/handle/10915/140773enginfo:eu-repo/semantics/altIdentifier/issn/1558-9307info:eu-repo/semantics/altIdentifier/issn/0024-4201info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-008-3277-zinfo:eu-repo/semantics/altIdentifier/pmid/19130109info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-29T15:37:13Zoai:sedici.unlp.edu.ar:10915/140773Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-29 15:37:13.678SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
title Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
spellingShingle Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
Hurtado de Catalfo, Graciela E.
Química
Medicina
Lipid composition
Fatty acids
Testosterone production
Testicular interstitial cells
Dietary lipids
title_short Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
title_full Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
title_fullStr Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
title_full_unstemmed Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
title_sort Influence of commercial dietary oils on lipid composition and testosterone production in interstitial cells isolated from rat testis
dc.creator.none.fl_str_mv Hurtado de Catalfo, Graciela E.
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author Hurtado de Catalfo, Graciela E.
author_facet Hurtado de Catalfo, Graciela E.
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author_role author
author2 Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author2_role author
author
dc.subject.none.fl_str_mv Química
Medicina
Lipid composition
Fatty acids
Testosterone production
Testicular interstitial cells
Dietary lipids
topic Química
Medicina
Lipid composition
Fatty acids
Testosterone production
Testicular interstitial cells
Dietary lipids
dc.description.none.fl_txt_mv The aim of this study was to examine the influence of dietary fat on lipid composition, as well as on the steroidogenic function of interstitial cells isolated from Wistar rats that had been fed semi-synthetic diets containing four different commercial oils (S soybean, O olive, C coconut, and G grape seed). Steroidogenic enzyme activities, lipid composition, and androgen production were measured in testicular interstitial cells. Lipid analysis included measurement of the contents of major lipid subclasses (neutral lipids, polar lipids, free and esterified cholesterol), as well as principal polar and neutral lipid fatty acyl compositions. Significant differences in lipid composition were observed among the groups, most of them reflecting the specific fatty acyl composition of the diet tested. Testosterone concentration was higher in O and C groups compared with S or G. In agreement with this observation, the activity of both key enzymes involved in testosterone biosynthesis (3-β-HSD and 17-β-HSD) was higher in O and C groups with significant differences between them (O > C). A significant negative correlation was found between cellular testosterone production and cellular cholesterol ester content. Additionally, testosterone concentration directly correlated with cholesterol levels. We conclude that dietary oils qualitatively and quantitatively modified the lipid composition of interstitial cells, producing either a direct or indirect regulatory effect on testicular steroidogenic function.
Instituto de Investigaciones Bioquímicas de La Plata
description The aim of this study was to examine the influence of dietary fat on lipid composition, as well as on the steroidogenic function of interstitial cells isolated from Wistar rats that had been fed semi-synthetic diets containing four different commercial oils (S soybean, O olive, C coconut, and G grape seed). Steroidogenic enzyme activities, lipid composition, and androgen production were measured in testicular interstitial cells. Lipid analysis included measurement of the contents of major lipid subclasses (neutral lipids, polar lipids, free and esterified cholesterol), as well as principal polar and neutral lipid fatty acyl compositions. Significant differences in lipid composition were observed among the groups, most of them reflecting the specific fatty acyl composition of the diet tested. Testosterone concentration was higher in O and C groups compared with S or G. In agreement with this observation, the activity of both key enzymes involved in testosterone biosynthesis (3-β-HSD and 17-β-HSD) was higher in O and C groups with significant differences between them (O > C). A significant negative correlation was found between cellular testosterone production and cellular cholesterol ester content. Additionally, testosterone concentration directly correlated with cholesterol levels. We conclude that dietary oils qualitatively and quantitatively modified the lipid composition of interstitial cells, producing either a direct or indirect regulatory effect on testicular steroidogenic function.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140773
url http://sedici.unlp.edu.ar/handle/10915/140773
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1558-9307
info:eu-repo/semantics/altIdentifier/issn/0024-4201
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-008-3277-z
info:eu-repo/semantics/altIdentifier/pmid/19130109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
345-357
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.10058

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