Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex

Autores
Restrepo Guerrero, Andrés Gonzalo; Goitía, Helen; Naso, Luciana Gissella; Rey, Marilin; Gonzalez, Pablo Javier; Ferrer, Evelina Gloria; Williams, Patricia Ana María
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).
Centro de Química Inorgánica
Materia
Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/155666

id SEDICI_792f0c952aa8e7946e8d4dc0d8dc5c0b
oai_identifier_str oai:sedici.unlp.edu.ar:10915/155666
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin ComplexRestrepo Guerrero, Andrés GonzaloGoitía, HelenNaso, Luciana GissellaRey, MarilinGonzalez, Pablo JavierFerrer, Evelina GloriaWilliams, Patricia Ana MaríaCiencias ExactasQuímicaglycosylated flavonoidoxidovanadium(IV) complexantitumoralantioxidantThe complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).Centro de Química Inorgánica2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/155666enginfo:eu-repo/semantics/altIdentifier/issn/2304-6740info:eu-repo/semantics/altIdentifier/doi/10.3390/inorganics10010013info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:40:26Zoai:sedici.unlp.edu.ar:10915/155666Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:40:26.856SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
spellingShingle Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
Restrepo Guerrero, Andrés Gonzalo
Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
title_short Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_full Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_fullStr Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_full_unstemmed Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_sort Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
dc.creator.none.fl_str_mv Restrepo Guerrero, Andrés Gonzalo
Goitía, Helen
Naso, Luciana Gissella
Rey, Marilin
Gonzalez, Pablo Javier
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author Restrepo Guerrero, Andrés Gonzalo
author_facet Restrepo Guerrero, Andrés Gonzalo
Goitía, Helen
Naso, Luciana Gissella
Rey, Marilin
Gonzalez, Pablo Javier
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author_role author
author2 Goitía, Helen
Naso, Luciana Gissella
Rey, Marilin
Gonzalez, Pablo Javier
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
topic Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
dc.description.none.fl_txt_mv The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).
Centro de Química Inorgánica
description The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/155666
url http://sedici.unlp.edu.ar/handle/10915/155666
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2304-6740
info:eu-repo/semantics/altIdentifier/doi/10.3390/inorganics10010013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616277227732992
score 13.070432