Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
- Autores
- Restrepo Guerrero, Andrés Gonzalo; Goitía, Helen; Naso, Luciana Gissella; Rey, Marilin; Gonzalez, Pablo Javier; Ferrer, Evelina Gloria; Williams, Patricia Ana María
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).
Centro de Química Inorgánica - Materia
-
Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/155666
Ver los metadatos del registro completo
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Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin ComplexRestrepo Guerrero, Andrés GonzaloGoitía, HelenNaso, Luciana GissellaRey, MarilinGonzalez, Pablo JavierFerrer, Evelina GloriaWilliams, Patricia Ana MaríaCiencias ExactasQuímicaglycosylated flavonoidoxidovanadium(IV) complexantitumoralantioxidantThe complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).Centro de Química Inorgánica2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/155666enginfo:eu-repo/semantics/altIdentifier/issn/2304-6740info:eu-repo/semantics/altIdentifier/doi/10.3390/inorganics10010013info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:40:26Zoai:sedici.unlp.edu.ar:10915/155666Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:40:26.856SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
title |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
spellingShingle |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex Restrepo Guerrero, Andrés Gonzalo Ciencias Exactas Química glycosylated flavonoid oxidovanadium(IV) complex antitumoral antioxidant |
title_short |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
title_full |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
title_fullStr |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
title_full_unstemmed |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
title_sort |
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex |
dc.creator.none.fl_str_mv |
Restrepo Guerrero, Andrés Gonzalo Goitía, Helen Naso, Luciana Gissella Rey, Marilin Gonzalez, Pablo Javier Ferrer, Evelina Gloria Williams, Patricia Ana María |
author |
Restrepo Guerrero, Andrés Gonzalo |
author_facet |
Restrepo Guerrero, Andrés Gonzalo Goitía, Helen Naso, Luciana Gissella Rey, Marilin Gonzalez, Pablo Javier Ferrer, Evelina Gloria Williams, Patricia Ana María |
author_role |
author |
author2 |
Goitía, Helen Naso, Luciana Gissella Rey, Marilin Gonzalez, Pablo Javier Ferrer, Evelina Gloria Williams, Patricia Ana María |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Química glycosylated flavonoid oxidovanadium(IV) complex antitumoral antioxidant |
topic |
Ciencias Exactas Química glycosylated flavonoid oxidovanadium(IV) complex antitumoral antioxidant |
dc.description.none.fl_txt_mv |
The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order). Centro de Química Inorgánica |
description |
The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order). |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/155666 |
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http://sedici.unlp.edu.ar/handle/10915/155666 |
dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/2304-6740 info:eu-repo/semantics/altIdentifier/doi/10.3390/inorganics10010013 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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