Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in a...

Autores
Muzzio, Nicolás Eduardo; Pasquale, Miguel Ángel; Diamanti, Eleftheria; Gregurec, Danijela; Martinez Moro, Marta; Azzaroni, Omar; Moya, Sergio E.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37 °C for 72 h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements. Cell adherence on PEMs before and after annealing was evaluated by measuring the cell spreading area and aspect ratio for the A549 epithelial, BHK kidney fibroblast, C2C12 myoblast and MC-3T3-E1 osteoblast cell lines. Chitosan/hyaluronic acid PEMs show a low cell adherence that decreases with the thermal annealing, as observed from the reduction in the average cell spreading area and more rounded cell morphology. The adhesion of S. aureus (Gram-positive) and E. coli (Gram-negative) bacteria strains was quantified by optical microscopy, counting the number of colony-forming units and measuring the light scattering of bacteria suspension after detachment from the PEM surface. A 20% decrease in bacteria adhesion was selectively observed in the S. aureus strain after annealing. The changes in mammalian cell and bacteria adhesion correlate with the changes in topography of the chitosan/hyaluronic PEMs from a rough fibrillar 3D structure to a smoother and planar surface after thermal annealing.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Ciencias Exactas
Bacteria adhesion Topography
Polyelectrolyte multilayers
Thermal annealing
Cell adhesion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/103462
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/103462
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteriaMuzzio, Nicolás EduardoPasquale, Miguel ÁngelDiamanti, EleftheriaGregurec, DanijelaMartinez Moro, MartaAzzaroni, OmarMoya, Sergio E.Ciencias ExactasBacteria adhesion TopographyPolyelectrolyte multilayersThermal annealingCell adhesionThe development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37 °C for 72 h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements. Cell adherence on PEMs before and after annealing was evaluated by measuring the cell spreading area and aspect ratio for the A549 epithelial, BHK kidney fibroblast, C2C12 myoblast and MC-3T3-E1 osteoblast cell lines. Chitosan/hyaluronic acid PEMs show a low cell adherence that decreases with the thermal annealing, as observed from the reduction in the average cell spreading area and more rounded cell morphology. The adhesion of <i>S. aureus</i> (Gram-positive) and <i>E. coli</i> (Gram-negative) bacteria strains was quantified by optical microscopy, counting the number of colony-forming units and measuring the light scattering of bacteria suspension after detachment from the PEM surface. A 20% decrease in bacteria adhesion was selectively observed in the <i>S. aureus</i> strain after annealing. The changes in mammalian cell and bacteria adhesion correlate with the changes in topography of the chitosan/hyaluronic PEMs from a rough fibrillar 3D structure to a smoother and planar surface after thermal annealing.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2017-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf677–687http://sedici.unlp.edu.ar/handle/10915/103462enginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0928493116323530?via%3Dihubinfo:eu-repo/semantics/altIdentifier/issn/0928-4931info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2017.07.016info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:14:24Zoai:sedici.unlp.edu.ar:10915/103462Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:14:24.636SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
title Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
spellingShingle Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
Muzzio, Nicolás Eduardo
Ciencias Exactas
Bacteria adhesion Topography
Polyelectrolyte multilayers
Thermal annealing
Cell adhesion
title_short Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
title_full Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
title_fullStr Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
title_full_unstemmed Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
title_sort Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing : Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
dc.creator.none.fl_str_mv Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Diamanti, Eleftheria
Gregurec, Danijela
Martinez Moro, Marta
Azzaroni, Omar
Moya, Sergio E.
author Muzzio, Nicolás Eduardo
author_facet Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
Diamanti, Eleftheria
Gregurec, Danijela
Martinez Moro, Marta
Azzaroni, Omar
Moya, Sergio E.
author_role author
author2 Pasquale, Miguel Ángel
Diamanti, Eleftheria
Gregurec, Danijela
Martinez Moro, Marta
Azzaroni, Omar
Moya, Sergio E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bacteria adhesion Topography
Polyelectrolyte multilayers
Thermal annealing
Cell adhesion
topic Ciencias Exactas
Bacteria adhesion Topography
Polyelectrolyte multilayers
Thermal annealing
Cell adhesion
dc.description.none.fl_txt_mv The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37 °C for 72 h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements. Cell adherence on PEMs before and after annealing was evaluated by measuring the cell spreading area and aspect ratio for the A549 epithelial, BHK kidney fibroblast, C2C12 myoblast and MC-3T3-E1 osteoblast cell lines. Chitosan/hyaluronic acid PEMs show a low cell adherence that decreases with the thermal annealing, as observed from the reduction in the average cell spreading area and more rounded cell morphology. The adhesion of <i>S. aureus</i> (Gram-positive) and <i>E. coli</i> (Gram-negative) bacteria strains was quantified by optical microscopy, counting the number of colony-forming units and measuring the light scattering of bacteria suspension after detachment from the PEM surface. A 20% decrease in bacteria adhesion was selectively observed in the <i>S. aureus</i> strain after annealing. The changes in mammalian cell and bacteria adhesion correlate with the changes in topography of the chitosan/hyaluronic PEMs from a rough fibrillar 3D structure to a smoother and planar surface after thermal annealing.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37 °C for 72 h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements. Cell adherence on PEMs before and after annealing was evaluated by measuring the cell spreading area and aspect ratio for the A549 epithelial, BHK kidney fibroblast, C2C12 myoblast and MC-3T3-E1 osteoblast cell lines. Chitosan/hyaluronic acid PEMs show a low cell adherence that decreases with the thermal annealing, as observed from the reduction in the average cell spreading area and more rounded cell morphology. The adhesion of <i>S. aureus</i> (Gram-positive) and <i>E. coli</i> (Gram-negative) bacteria strains was quantified by optical microscopy, counting the number of colony-forming units and measuring the light scattering of bacteria suspension after detachment from the PEM surface. A 20% decrease in bacteria adhesion was selectively observed in the <i>S. aureus</i> strain after annealing. The changes in mammalian cell and bacteria adhesion correlate with the changes in topography of the chitosan/hyaluronic PEMs from a rough fibrillar 3D structure to a smoother and planar surface after thermal annealing.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/103462
url http://sedici.unlp.edu.ar/handle/10915/103462
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/0928-4931
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2017.07.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
677–687
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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