Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion

Autores
Muzzio, Nicolás Eduardo; Gregurec, Danijela; Diamanti, Eleftheria; Irigoyen, Joseba; Pasquale, Miguel Angel; Azzaroni, Omar; Moya, Sergio Eduardo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyelectrolyte multilayers (PEMs) have many potential applications in tissue engineering and regenerative medicine. However, the softness of biocompatible PEMs results in limited cell adhesion. A novel strategy for the enhancement of cell adhesion on PEMs based on thermal annealing is presented here. The impact of thermal annealing at 37 ºC of poly-l-lysine (PLL) and alginate (Alg) polyelectrolyte multilayers on the adhesion of human lung cancer A549 and myoblast C2C12 cell lines is studied. The properties of the PEMs after annealing are characterized by means of the quartz crystal microbalance with dissipation, atomic force microscopy, atomic force spectroscopy, zeta potential, and contact angle measurements. After annealing, PLL/Alg PEMs become smoother displaying an increase in stiffness. Furthermore, PEMs become more hydrophobic, with an increase in contact angle from 36° to 90°. Additionally, the surface charge decreases and protein deposition on PEMs significantly diminishes after annealing. Cell adhesion, measured by the projected average cell spreading and focal contact formation, is remarkably improved for the annealed PEMs.
Fil: Muzzio, Nicolás Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Gregurec, Danijela. CIC bioma; España
Fil: Diamanti, Eleftheria. CIC bioma; España
Fil: Irigoyen, Joseba. CIC bioma; España
Fil: Pasquale, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Moya, Sergio Eduardo. CIC bioma; España
Materia
Polyelectrolyte Multilayers
Thermal Annealing
Cell Adhesion
Antifouling
Contact Angle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61779
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61779
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell AdhesionMuzzio, Nicolás EduardoGregurec, DanijelaDiamanti, EleftheriaIrigoyen, JosebaPasquale, Miguel AngelAzzaroni, OmarMoya, Sergio EduardoPolyelectrolyte MultilayersThermal AnnealingCell AdhesionAntifoulingContact Anglehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Polyelectrolyte multilayers (PEMs) have many potential applications in tissue engineering and regenerative medicine. However, the softness of biocompatible PEMs results in limited cell adhesion. A novel strategy for the enhancement of cell adhesion on PEMs based on thermal annealing is presented here. The impact of thermal annealing at 37 ºC of poly-l-lysine (PLL) and alginate (Alg) polyelectrolyte multilayers on the adhesion of human lung cancer A549 and myoblast C2C12 cell lines is studied. The properties of the PEMs after annealing are characterized by means of the quartz crystal microbalance with dissipation, atomic force microscopy, atomic force spectroscopy, zeta potential, and contact angle measurements. After annealing, PLL/Alg PEMs become smoother displaying an increase in stiffness. Furthermore, PEMs become more hydrophobic, with an increase in contact angle from 36° to 90°. Additionally, the surface charge decreases and protein deposition on PEMs significantly diminishes after annealing. Cell adhesion, measured by the projected average cell spreading and focal contact formation, is remarkably improved for the annealed PEMs.Fil: Muzzio, Nicolás Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Gregurec, Danijela. CIC bioma; EspañaFil: Diamanti, Eleftheria. CIC bioma; EspañaFil: Irigoyen, Joseba. CIC bioma; EspañaFil: Pasquale, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Moya, Sergio Eduardo. CIC bioma; EspañaWiley-VCH Verlag2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/61779Muzzio, Nicolás Eduardo; Gregurec, Danijela; Diamanti, Eleftheria; Irigoyen, Joseba; Pasquale, Miguel Angel; et al.; Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion; Wiley-VCH Verlag; Advanced Materials Interfaces; 4; 1; 1-20172196-7350CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/admi.201600126info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/admi.201600126info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:26Zoai:ri.conicet.gov.ar:11336/61779instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:27.205CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
title Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
spellingShingle Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
Muzzio, Nicolás Eduardo
Polyelectrolyte Multilayers
Thermal Annealing
Cell Adhesion
Antifouling
Contact Angle
title_short Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
title_full Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
title_fullStr Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
title_full_unstemmed Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
title_sort Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion
dc.creator.none.fl_str_mv Muzzio, Nicolás Eduardo
Gregurec, Danijela
Diamanti, Eleftheria
Irigoyen, Joseba
Pasquale, Miguel Angel
Azzaroni, Omar
Moya, Sergio Eduardo
author Muzzio, Nicolás Eduardo
author_facet Muzzio, Nicolás Eduardo
Gregurec, Danijela
Diamanti, Eleftheria
Irigoyen, Joseba
Pasquale, Miguel Angel
Azzaroni, Omar
Moya, Sergio Eduardo
author_role author
author2 Gregurec, Danijela
Diamanti, Eleftheria
Irigoyen, Joseba
Pasquale, Miguel Angel
Azzaroni, Omar
Moya, Sergio Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Polyelectrolyte Multilayers
Thermal Annealing
Cell Adhesion
Antifouling
Contact Angle
topic Polyelectrolyte Multilayers
Thermal Annealing
Cell Adhesion
Antifouling
Contact Angle
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyelectrolyte multilayers (PEMs) have many potential applications in tissue engineering and regenerative medicine. However, the softness of biocompatible PEMs results in limited cell adhesion. A novel strategy for the enhancement of cell adhesion on PEMs based on thermal annealing is presented here. The impact of thermal annealing at 37 ºC of poly-l-lysine (PLL) and alginate (Alg) polyelectrolyte multilayers on the adhesion of human lung cancer A549 and myoblast C2C12 cell lines is studied. The properties of the PEMs after annealing are characterized by means of the quartz crystal microbalance with dissipation, atomic force microscopy, atomic force spectroscopy, zeta potential, and contact angle measurements. After annealing, PLL/Alg PEMs become smoother displaying an increase in stiffness. Furthermore, PEMs become more hydrophobic, with an increase in contact angle from 36° to 90°. Additionally, the surface charge decreases and protein deposition on PEMs significantly diminishes after annealing. Cell adhesion, measured by the projected average cell spreading and focal contact formation, is remarkably improved for the annealed PEMs.
Fil: Muzzio, Nicolás Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Gregurec, Danijela. CIC bioma; España
Fil: Diamanti, Eleftheria. CIC bioma; España
Fil: Irigoyen, Joseba. CIC bioma; España
Fil: Pasquale, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Moya, Sergio Eduardo. CIC bioma; España
description Polyelectrolyte multilayers (PEMs) have many potential applications in tissue engineering and regenerative medicine. However, the softness of biocompatible PEMs results in limited cell adhesion. A novel strategy for the enhancement of cell adhesion on PEMs based on thermal annealing is presented here. The impact of thermal annealing at 37 ºC of poly-l-lysine (PLL) and alginate (Alg) polyelectrolyte multilayers on the adhesion of human lung cancer A549 and myoblast C2C12 cell lines is studied. The properties of the PEMs after annealing are characterized by means of the quartz crystal microbalance with dissipation, atomic force microscopy, atomic force spectroscopy, zeta potential, and contact angle measurements. After annealing, PLL/Alg PEMs become smoother displaying an increase in stiffness. Furthermore, PEMs become more hydrophobic, with an increase in contact angle from 36° to 90°. Additionally, the surface charge decreases and protein deposition on PEMs significantly diminishes after annealing. Cell adhesion, measured by the projected average cell spreading and focal contact formation, is remarkably improved for the annealed PEMs.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61779
Muzzio, Nicolás Eduardo; Gregurec, Danijela; Diamanti, Eleftheria; Irigoyen, Joseba; Pasquale, Miguel Angel; et al.; Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion; Wiley-VCH Verlag; Advanced Materials Interfaces; 4; 1; 1-2017
2196-7350
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61779
identifier_str_mv Muzzio, Nicolás Eduardo; Gregurec, Danijela; Diamanti, Eleftheria; Irigoyen, Joseba; Pasquale, Miguel Angel; et al.; Thermal Annealing of Polyelectrolyte Multilayers: An Effective Approach for the Enhancement of Cell Adhesion; Wiley-VCH Verlag; Advanced Materials Interfaces; 4; 1; 1-2017
2196-7350
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/admi.201600126
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/admi.201600126
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH Verlag
publisher.none.fl_str_mv Wiley-VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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