Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals

Autores
Pedrazzini, Estela Maris; Mamaev, Nikolai; Slavutsky, Irma
Año de publicación
1998
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p < 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.
Facultad de Ciencias Exactas
Materia
Biología
Nucleolar organiser regions
Silver staining
Healthy human beings
Aging
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107698

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network_name_str SEDICI (UNLP)
spelling Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individualsPedrazzini, Estela MarisMamaev, NikolaiSlavutsky, IrmaBiologíaNucleolar organiser regionsSilver stainingHealthy human beingsAgingAims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p &lt; 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.Facultad de Ciencias Exactas1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf39-42http://sedici.unlp.edu.ar/handle/10915/107698enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC395607&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/1366-8714info:eu-repo/semantics/altIdentifier/pmid/9624419info:eu-repo/semantics/altIdentifier/doi/10.1136/mp.51.1.39info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:23:56Zoai:sedici.unlp.edu.ar:10915/107698Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:23:56.939SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
title Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
spellingShingle Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
Pedrazzini, Estela Maris
Biología
Nucleolar organiser regions
Silver staining
Healthy human beings
Aging
title_short Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
title_full Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
title_fullStr Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
title_full_unstemmed Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
title_sort Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
dc.creator.none.fl_str_mv Pedrazzini, Estela Maris
Mamaev, Nikolai
Slavutsky, Irma
author Pedrazzini, Estela Maris
author_facet Pedrazzini, Estela Maris
Mamaev, Nikolai
Slavutsky, Irma
author_role author
author2 Mamaev, Nikolai
Slavutsky, Irma
author2_role author
author
dc.subject.none.fl_str_mv Biología
Nucleolar organiser regions
Silver staining
Healthy human beings
Aging
topic Biología
Nucleolar organiser regions
Silver staining
Healthy human beings
Aging
dc.description.none.fl_txt_mv Aims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p &lt; 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.
Facultad de Ciencias Exactas
description Aims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p &lt; 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.
publishDate 1998
dc.date.none.fl_str_mv 1998
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107698
url http://sedici.unlp.edu.ar/handle/10915/107698
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC395607&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/1366-8714
info:eu-repo/semantics/altIdentifier/pmid/9624419
info:eu-repo/semantics/altIdentifier/doi/10.1136/mp.51.1.39
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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