Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals
- Autores
- Pedrazzini, Estela Maris; Mamaev, Nikolai; Slavutsky, Irma
- Año de publicación
- 1998
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p < 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.
Facultad de Ciencias Exactas - Materia
-
Biología
Nucleolar organiser regions
Silver staining
Healthy human beings
Aging - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/107698
Ver los metadatos del registro completo
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Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individualsPedrazzini, Estela MarisMamaev, NikolaiSlavutsky, IrmaBiologíaNucleolar organiser regionsSilver stainingHealthy human beingsAgingAims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p < 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.Facultad de Ciencias Exactas1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf39-42http://sedici.unlp.edu.ar/handle/10915/107698enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC395607&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/1366-8714info:eu-repo/semantics/altIdentifier/pmid/9624419info:eu-repo/semantics/altIdentifier/doi/10.1136/mp.51.1.39info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:23:56Zoai:sedici.unlp.edu.ar:10915/107698Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:23:56.939SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
title |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
spellingShingle |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals Pedrazzini, Estela Maris Biología Nucleolar organiser regions Silver staining Healthy human beings Aging |
title_short |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
title_full |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
title_fullStr |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
title_full_unstemmed |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
title_sort |
Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals |
dc.creator.none.fl_str_mv |
Pedrazzini, Estela Maris Mamaev, Nikolai Slavutsky, Irma |
author |
Pedrazzini, Estela Maris |
author_facet |
Pedrazzini, Estela Maris Mamaev, Nikolai Slavutsky, Irma |
author_role |
author |
author2 |
Mamaev, Nikolai Slavutsky, Irma |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Biología Nucleolar organiser regions Silver staining Healthy human beings Aging |
topic |
Biología Nucleolar organiser regions Silver staining Healthy human beings Aging |
dc.description.none.fl_txt_mv |
Aims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p < 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss. Facultad de Ciencias Exactas |
description |
Aims—To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor. Methods—Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37°C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall’s rank correlation coeYcient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells. Results—The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant diVerences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = −0.57 (p < 0.0012). Conclusions—The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/107698 |
url |
http://sedici.unlp.edu.ar/handle/10915/107698 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC395607&blobtype=pdf info:eu-repo/semantics/altIdentifier/issn/1366-8714 info:eu-repo/semantics/altIdentifier/pmid/9624419 info:eu-repo/semantics/altIdentifier/doi/10.1136/mp.51.1.39 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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