Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus

Autores
Anastasi, A.; Erspamer, V.; Cei, José Miguel Alfredo María
Año de publicación
1964
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that physalaemin is an endecapeptide having the following amino acid composition and sequence: Pyr-Ala-Asp(OH)-Pro-Asp(NH2)-Lys-Phe-Tyr-Gly-Leu-Met-NH2 Some anomalies in the trypsin and chymotrypsin fission of physalaemin are discussed, and the strict chemical resemblance between physalaemin and eledoisin is pointed out.
Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).
Facultad de Ciencias Naturales y Museo
Materia
Zoología
Anfibios
Polypeptide physalaemin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/89935
Acceder
id SEDICI_722013fe5fba970e17f22977531f854c
oai_identifier_str oai:sedici.unlp.edu.ar:10915/89935
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatusAnastasi, A.Erspamer, V.Cei, José Miguel Alfredo MaríaZoologíaAnfibiosPolypeptide physalaeminCrude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that physalaemin is an endecapeptide having the following amino acid composition and sequence: Pyr-Ala-Asp(OH)-Pro-Asp(NH2)-Lys-Phe-Tyr-Gly-Leu-Met-NH2 Some anomalies in the trypsin and chymotrypsin fission of physalaemin are discussed, and the strict chemical resemblance between physalaemin and eledoisin is pointed out.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Museo1964-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf341-348http://sedici.unlp.edu.ar/handle/10915/89935enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:18:23Zoai:sedici.unlp.edu.ar:10915/89935Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:18:24.022SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
spellingShingle Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
Anastasi, A.
Zoología
Anfibios
Polypeptide physalaemin
title_short Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_full Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_fullStr Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_full_unstemmed Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
title_sort Isolation and Amino Acid Sequence of Physalaemin, the Main Active Polypeptide of the Skin of Physalaemus fuscumaculatus
dc.creator.none.fl_str_mv Anastasi, A.
Erspamer, V.
Cei, José Miguel Alfredo María
author Anastasi, A.
author_facet Anastasi, A.
Erspamer, V.
Cei, José Miguel Alfredo María
author_role author
author2 Erspamer, V.
Cei, José Miguel Alfredo María
author2_role author
author
dc.subject.none.fl_str_mv Zoología
Anfibios
Polypeptide physalaemin
topic Zoología
Anfibios
Polypeptide physalaemin
dc.description.none.fl_txt_mv Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that physalaemin is an endecapeptide having the following amino acid composition and sequence: Pyr-Ala-Asp(OH)-Pro-Asp(NH2)-Lys-Phe-Tyr-Gly-Leu-Met-NH2 Some anomalies in the trypsin and chymotrypsin fission of physalaemin are discussed, and the strict chemical resemblance between physalaemin and eledoisin is pointed out.
Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).
Facultad de Ciencias Naturales y Museo
description Crude methanol extracts of fresh and dried skins of Physalaemus fuscumaculatus, a South American amphibian, were subjected successively to chromatography on alkaline alumina and to countercurrent distribution. The main active constituent of these extracts, the polypeptide physalaemin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that physalaemin is an endecapeptide having the following amino acid composition and sequence: Pyr-Ala-Asp(OH)-Pro-Asp(NH2)-Lys-Phe-Tyr-Gly-Leu-Met-NH2 Some anomalies in the trypsin and chymotrypsin fission of physalaemin are discussed, and the strict chemical resemblance between physalaemin and eledoisin is pointed out.
publishDate 1964
dc.date.none.fl_str_mv 1964-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/89935
url http://sedici.unlp.edu.ar/handle/10915/89935
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
341-348
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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