Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina

Autores
De Felice, Lorena Alejandra; Moré, Gastón Andrés; Cappuccio, Javier Alejandro; Venturini, María Cecilia; Unzaga, Juan Manuel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.
Laboratorio de Inmunoparasitología
Consejo Nacional de Investigaciones Científicas y Técnicas
Instituto Nacional de Tecnología Agropecuaria
Materia
Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/150309
Acceder
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network_name_str SEDICI (UNLP)
spelling Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in ArgentinaDe Felice, Lorena AlejandraMoré, Gastón AndrésCappuccio, Javier AlejandroVenturini, María CeciliaUnzaga, Juan ManuelCiencias VeterinariasCryptosporidium suisCryptosporidium scrofarumPigletsOocystsMicroscopyNested PCRCryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.Laboratorio de InmunoparasitologíaConsejo Nacional de Investigaciones Científicas y TécnicasInstituto Nacional de Tecnología Agropecuaria2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/150309enginfo:eu-repo/semantics/altIdentifier/issn/2405-9390info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vprsr.2020.100473info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:30:26Zoai:sedici.unlp.edu.ar:10915/150309Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:30:26.496SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
spellingShingle Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
De Felice, Lorena Alejandra
Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
title_short Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_full Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_fullStr Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_full_unstemmed Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_sort Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
dc.creator.none.fl_str_mv De Felice, Lorena Alejandra
Moré, Gastón Andrés
Cappuccio, Javier Alejandro
Venturini, María Cecilia
Unzaga, Juan Manuel
author De Felice, Lorena Alejandra
author_facet De Felice, Lorena Alejandra
Moré, Gastón Andrés
Cappuccio, Javier Alejandro
Venturini, María Cecilia
Unzaga, Juan Manuel
author_role author
author2 Moré, Gastón Andrés
Cappuccio, Javier Alejandro
Venturini, María Cecilia
Unzaga, Juan Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
topic Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
dc.description.none.fl_txt_mv Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.
Laboratorio de Inmunoparasitología
Consejo Nacional de Investigaciones Científicas y Técnicas
Instituto Nacional de Tecnología Agropecuaria
description Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/150309
url http://sedici.unlp.edu.ar/handle/10915/150309
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2405-9390
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vprsr.2020.100473
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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