The positive inotropic effect of angiotensin II : Role of endothelin-1 and reactive oxygen species

• Autores: Cingolani, Horacio Eugenio; Villa Abrille, María Celeste; Cornelli, Mariana; Nolly, Alejandro; Ennis, Irene Lucía; Garciarena, Carolina Denis; Suburo, Angela M.; Torbidoni, Vanesa; Correa, María Verónica; Camilión de Hurtado, María Cristina; Aiello, Ernesto Alejandro
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Ciencias Médicas; Ion channels; Membranas; Oxidative stress; Receptores de Angiotensina; Angiotensina II
• Institución: Universidad Nacional de La Plata

Many effects believed to be because of angiotensin II (Ang II) are attributable to the action of endothelin (ET)-1, which is released/produced by Ang II. We investigated whether Ang II elicits its positive inotropic effect (PIE) by the action of endogenous ET-1, in addition to the role played by reactive oxygen species (ROS) in this mechanism. Cat cardiomyocytes were used for: (1) sarcomere shortening measurements; (2) ROS measurements by epifluorescence; (3) immunohistochemical staining for preproET-1, BigET-1, and ET-1; and (4) measurement of preproET-1 mRNA by RT-PCR. Cells were exposed to 1 nmol/L Ang II for 15 minutes. This low concentration of Ang II increases sarcomere shortening by 29.2±3.7% (P<0.05). This PIE was abrogated by Na+/H+ exchanger or Na+/Ca2+ exchanger reverse mode inhibition. The production of ROS increased in response to Ang II treatment (ΔROS respect to control: 68±15 fluorescence units; P<0.05). The Ang II-induced PIE and ROS production were blocked by the Ang II type 1 receptor blocker losartan, the nonselective ET-1 receptor blocker TAK044, the selective ETA receptor blocker BQ-123, or the ROS scavenger N-(2-mercapto-propionyl)glycine. Exogenous ET-1 (0.4 nmol/L) induced a similar PIE and increase in ROS production to those caused by Ang II. Immunostaining for preproET-1, BigET-1, and ET-I was positive in cardiomyocytes. The preproET-1 mRNA abundance increased from 100±4.6% in control to 241.9±39.9% in Ang II-treated cells (P<0.05). We conclude that the PIE after exposure to 1 nmol/L Ang II is due to endogenous ET-1 acting through the ETA receptor and triggering ROS production, Na+/H+ exchanger stimulation, and Na+/Ca2+ exchanger reverse mode activation.
Facultad de Ciencias Médicas