Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reacti...
- Autores
- Smaldini, Paola Lorena; Orsini Delgado, María Lucía; Fossati, Carlos Alberto; Docena, Guillermo Horacio
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4+ CD25+-FoxP3+ cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.
Facultad de Ciencias Exactas
Instituto de Estudios Inmunológicos y Fisiopatológicos - Materia
-
Ciencias Exactas
Oral immunotherapy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/86454
Ver los metadatos del registro completo
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Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactionsSmaldini, Paola LorenaOrsini Delgado, María LucíaFossati, Carlos AlbertoDocena, Guillermo HoracioCiencias ExactasOral immunotherapyThe induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.Facultad de Ciencias ExactasInstituto de Estudios Inmunológicos y Fisiopatológicos2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86454enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0141116info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:12Zoai:sedici.unlp.edu.ar:10915/86454Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:12.698SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
title |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
spellingShingle |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions Smaldini, Paola Lorena Ciencias Exactas Oral immunotherapy |
title_short |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
title_full |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
title_fullStr |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
title_full_unstemmed |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
title_sort |
Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions |
dc.creator.none.fl_str_mv |
Smaldini, Paola Lorena Orsini Delgado, María Lucía Fossati, Carlos Alberto Docena, Guillermo Horacio |
author |
Smaldini, Paola Lorena |
author_facet |
Smaldini, Paola Lorena Orsini Delgado, María Lucía Fossati, Carlos Alberto Docena, Guillermo Horacio |
author_role |
author |
author2 |
Orsini Delgado, María Lucía Fossati, Carlos Alberto Docena, Guillermo Horacio |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Oral immunotherapy |
topic |
Ciencias Exactas Oral immunotherapy |
dc.description.none.fl_txt_mv |
The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response. Facultad de Ciencias Exactas Instituto de Estudios Inmunológicos y Fisiopatológicos |
description |
The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/86454 |
url |
http://sedici.unlp.edu.ar/handle/10915/86454 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1932-6203 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0141116 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf |
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