Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reacti...

Autores
Smaldini, Paola Lorena; Orsini Delgado, María Lucía; Fossati, Carlos Alberto; Docena, Guillermo Horacio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4+ CD25+-FoxP3+ cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.
Facultad de Ciencias Exactas
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Ciencias Exactas
Oral immunotherapy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86454

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network_name_str SEDICI (UNLP)
spelling Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactionsSmaldini, Paola LorenaOrsini Delgado, María LucíaFossati, Carlos AlbertoDocena, Guillermo HoracioCiencias ExactasOral immunotherapyThe induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.Facultad de Ciencias ExactasInstituto de Estudios Inmunológicos y Fisiopatológicos2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86454enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0141116info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:12Zoai:sedici.unlp.edu.ar:10915/86454Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:12.698SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
spellingShingle Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
Smaldini, Paola Lorena
Ciencias Exactas
Oral immunotherapy
title_short Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_full Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_fullStr Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_full_unstemmed Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
title_sort Orally-induced intestinal CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> treg controlled undesired responses towards oral antigens and effectively dampened food allergic reactions
dc.creator.none.fl_str_mv Smaldini, Paola Lorena
Orsini Delgado, María Lucía
Fossati, Carlos Alberto
Docena, Guillermo Horacio
author Smaldini, Paola Lorena
author_facet Smaldini, Paola Lorena
Orsini Delgado, María Lucía
Fossati, Carlos Alberto
Docena, Guillermo Horacio
author_role author
author2 Orsini Delgado, María Lucía
Fossati, Carlos Alberto
Docena, Guillermo Horacio
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Oral immunotherapy
topic Ciencias Exactas
Oral immunotherapy
dc.description.none.fl_txt_mv The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.
Facultad de Ciencias Exactas
Instituto de Estudios Inmunológicos y Fisiopatológicos
description The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus milk proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 gene expression in jejunum was down-regulated, and IL-10 and TGF-β were increased. In addition, we observed an induction of CD4<sup>+</sup> CD25<sup>+-</sup>FoxP3<sup>+</sup> cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria. Finally, transfer experiments confirmed the central role of these cells in tolerance induction. We demonstrated that the oral administration of milk proteins pre- or post-sensitization controlled the Th2-immune response through the elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibited hypersensitivity symptoms and the allergic response.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0141116
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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reponame_str SEDICI (UNLP)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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