A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina

Autores
Medina, Rocío; López, Silvina Marianela Yanil; Franco, Mario Emilio Ernesto; Rollán, María Cristina; Ronco, Blanca Lía; Saparrat, Mario Carlos Nazareno; De Wit, Pierre J. G. M.; Balatti, Pedro Alberto
Año de publicación
2015
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The presence of Cladosporium fulvum (syn. Passalora fulva), causal agent of tomato leaf mold, was confirmed in the two main greenhouseproduction areas for tomato in Argentina. Using both morphological characters and internal transcribed spacer sequencing, we confirmed the presence of physiological races of this pathogen. A diagnostic multiplex polymerase chain reaction (PCR) was also developed, using primers derived from C. fulvum avirulence (Avr) genes. In all, 20 isolates of Cladosporium spp. were obtained as monospore cultures and 12 were identified as C. fulvum. By this method, we showed that, of these 12 isolates, 5 were race 0 (carrying functional Avr2, Avr4, Avr4E, and Avr9 genes) and 7 were race 2 (lacking the Avr2 gene). Race identity was confirmed by testing their virulence on a set of tomato differentials carrying different Cf resistance genes. All Avr genes could be amplified in single or multiplex PCR using DNA isolated from in vitro grown monospore cultures but only three Avr could be amplified when genomic DNA was isolated from C. fulvum-infected necrotic leaf tissue.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Enfermedades de las Plantas
Cultivos Agrícolas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/66622
Acceder
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network_name_str SEDICI (UNLP)
spelling A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of ArgentinaMedina, RocíoLópez, Silvina Marianela YanilFranco, Mario Emilio ErnestoRollán, María CristinaRonco, Blanca LíaSaparrat, Mario Carlos NazarenoDe Wit, Pierre J. G. M.Balatti, Pedro AlbertoCiencias AgrariasEnfermedades de las PlantasCultivos AgrícolasThe presence of Cladosporium fulvum (syn. Passalora fulva), causal agent of tomato leaf mold, was confirmed in the two main greenhouseproduction areas for tomato in Argentina. Using both morphological characters and internal transcribed spacer sequencing, we confirmed the presence of physiological races of this pathogen. A diagnostic multiplex polymerase chain reaction (PCR) was also developed, using primers derived from C. fulvum avirulence (Avr) genes. In all, 20 isolates of Cladosporium spp. were obtained as monospore cultures and 12 were identified as C. fulvum. By this method, we showed that, of these 12 isolates, 5 were race 0 (carrying functional Avr2, Avr4, Avr4E, and Avr9 genes) and 7 were race 2 (lacking the Avr2 gene). Race identity was confirmed by testing their virulence on a set of tomato differentials carrying different Cf resistance genes. All Avr genes could be amplified in single or multiplex PCR using DNA isolated from in vitro grown monospore cultures but only three Avr could be amplified when genomic DNA was isolated from C. fulvum-infected necrotic leaf tissue.Facultad de Ciencias Agrarias y Forestales2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1732-1737http://sedici.unlp.edu.ar/handle/10915/66622spainfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-12-14-1270-REinfo:eu-repo/semantics/altIdentifier/issn/1943-7692info:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-12-14-1270-REinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:42:04Zoai:sedici.unlp.edu.ar:10915/66622Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:42:04.953SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
title A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
spellingShingle A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
Medina, Rocío
Ciencias Agrarias
Enfermedades de las Plantas
Cultivos Agrícolas
title_short A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
title_full A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
title_fullStr A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
title_full_unstemmed A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
title_sort A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina
dc.creator.none.fl_str_mv Medina, Rocío
López, Silvina Marianela Yanil
Franco, Mario Emilio Ernesto
Rollán, María Cristina
Ronco, Blanca Lía
Saparrat, Mario Carlos Nazareno
De Wit, Pierre J. G. M.
Balatti, Pedro Alberto
author Medina, Rocío
author_facet Medina, Rocío
López, Silvina Marianela Yanil
Franco, Mario Emilio Ernesto
Rollán, María Cristina
Ronco, Blanca Lía
Saparrat, Mario Carlos Nazareno
De Wit, Pierre J. G. M.
Balatti, Pedro Alberto
author_role author
author2 López, Silvina Marianela Yanil
Franco, Mario Emilio Ernesto
Rollán, María Cristina
Ronco, Blanca Lía
Saparrat, Mario Carlos Nazareno
De Wit, Pierre J. G. M.
Balatti, Pedro Alberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Enfermedades de las Plantas
Cultivos Agrícolas
topic Ciencias Agrarias
Enfermedades de las Plantas
Cultivos Agrícolas
dc.description.none.fl_txt_mv The presence of Cladosporium fulvum (syn. Passalora fulva), causal agent of tomato leaf mold, was confirmed in the two main greenhouseproduction areas for tomato in Argentina. Using both morphological characters and internal transcribed spacer sequencing, we confirmed the presence of physiological races of this pathogen. A diagnostic multiplex polymerase chain reaction (PCR) was also developed, using primers derived from C. fulvum avirulence (Avr) genes. In all, 20 isolates of Cladosporium spp. were obtained as monospore cultures and 12 were identified as C. fulvum. By this method, we showed that, of these 12 isolates, 5 were race 0 (carrying functional Avr2, Avr4, Avr4E, and Avr9 genes) and 7 were race 2 (lacking the Avr2 gene). Race identity was confirmed by testing their virulence on a set of tomato differentials carrying different Cf resistance genes. All Avr genes could be amplified in single or multiplex PCR using DNA isolated from in vitro grown monospore cultures but only three Avr could be amplified when genomic DNA was isolated from C. fulvum-infected necrotic leaf tissue.
Facultad de Ciencias Agrarias y Forestales
description The presence of Cladosporium fulvum (syn. Passalora fulva), causal agent of tomato leaf mold, was confirmed in the two main greenhouseproduction areas for tomato in Argentina. Using both morphological characters and internal transcribed spacer sequencing, we confirmed the presence of physiological races of this pathogen. A diagnostic multiplex polymerase chain reaction (PCR) was also developed, using primers derived from C. fulvum avirulence (Avr) genes. In all, 20 isolates of Cladosporium spp. were obtained as monospore cultures and 12 were identified as C. fulvum. By this method, we showed that, of these 12 isolates, 5 were race 0 (carrying functional Avr2, Avr4, Avr4E, and Avr9 genes) and 7 were race 2 (lacking the Avr2 gene). Race identity was confirmed by testing their virulence on a set of tomato differentials carrying different Cf resistance genes. All Avr genes could be amplified in single or multiplex PCR using DNA isolated from in vitro grown monospore cultures but only three Avr could be amplified when genomic DNA was isolated from C. fulvum-infected necrotic leaf tissue.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/66622
url http://sedici.unlp.edu.ar/handle/10915/66622
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-12-14-1270-RE
info:eu-repo/semantics/altIdentifier/issn/1943-7692
info:eu-repo/semantics/altIdentifier/doi/10.1094/PDIS-12-14-1270-RE
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
1732-1737
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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