Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment

Autores
Cacicedo, Maximiliano Luis; Islan, Germán Abel; León, Ignacio Esteban; Alvarez, Vera Alejandra; Chourpa, Igor; Allard Vannier, E.; García Aranda, N.; Díaz Riascos, Z. V.; Fernández, Y.; Schwartz, S.; Abasolo, Ibane; Castro, Guillermo Raúl
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery. This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects. We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model. NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells. Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model. NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release. Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox. Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Centro de Química Inorgánica
Materia
Química
Bacterial cellulose
Drug delivery
Bacterial cellulose
Doxorubicin
Breast cancer
Hydrogel
Localized chemotherapy
Nanocomposite
Nanostructured lipid carriers
Neo-adjuvant therapy
Controlled release
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/96219
Acceder
id SEDICI_6e3650142d13c5111f900fb6b4be48f6
oai_identifier_str oai:sedici.unlp.edu.ar:10915/96219
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatmentCacicedo, Maximiliano LuisIslan, Germán AbelLeón, Ignacio EstebanAlvarez, Vera AlejandraChourpa, IgorAllard Vannier, E.García Aranda, N.Díaz Riascos, Z. V.Fernández, Y.Schwartz, S.Abasolo, IbaneCastro, Guillermo RaúlQuímicaBacterial celluloseDrug deliveryBacterial celluloseDoxorubicinBreast cancerHydrogelLocalized chemotherapyNanocompositeNanostructured lipid carriersNeo-adjuvant therapyControlled releaseThe use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery. This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects. We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model. NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells. Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model. NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release. Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox. Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesCentro de Química Inorgánica2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf596-608http://sedici.unlp.edu.ar/handle/10915/96219enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/91815info:eu-repo/semantics/altIdentifier/issn/0927-7765info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2018.06.056info:eu-repo/semantics/altIdentifier/hdl/11336/91815info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-10T12:23:14Zoai:sedici.unlp.edu.ar:10915/96219Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-10 12:23:15.258SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
title Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
spellingShingle Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
Cacicedo, Maximiliano Luis
Química
Bacterial cellulose
Drug delivery
Bacterial cellulose
Doxorubicin
Breast cancer
Hydrogel
Localized chemotherapy
Nanocomposite
Nanostructured lipid carriers
Neo-adjuvant therapy
Controlled release
title_short Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
title_full Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
title_fullStr Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
title_full_unstemmed Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
title_sort Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
dc.creator.none.fl_str_mv Cacicedo, Maximiliano Luis
Islan, Germán Abel
León, Ignacio Esteban
Alvarez, Vera Alejandra
Chourpa, Igor
Allard Vannier, E.
García Aranda, N.
Díaz Riascos, Z. V.
Fernández, Y.
Schwartz, S.
Abasolo, Ibane
Castro, Guillermo Raúl
author Cacicedo, Maximiliano Luis
author_facet Cacicedo, Maximiliano Luis
Islan, Germán Abel
León, Ignacio Esteban
Alvarez, Vera Alejandra
Chourpa, Igor
Allard Vannier, E.
García Aranda, N.
Díaz Riascos, Z. V.
Fernández, Y.
Schwartz, S.
Abasolo, Ibane
Castro, Guillermo Raúl
author_role author
author2 Islan, Germán Abel
León, Ignacio Esteban
Alvarez, Vera Alejandra
Chourpa, Igor
Allard Vannier, E.
García Aranda, N.
Díaz Riascos, Z. V.
Fernández, Y.
Schwartz, S.
Abasolo, Ibane
Castro, Guillermo Raúl
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
Bacterial cellulose
Drug delivery
Bacterial cellulose
Doxorubicin
Breast cancer
Hydrogel
Localized chemotherapy
Nanocomposite
Nanostructured lipid carriers
Neo-adjuvant therapy
Controlled release
topic Química
Bacterial cellulose
Drug delivery
Bacterial cellulose
Doxorubicin
Breast cancer
Hydrogel
Localized chemotherapy
Nanocomposite
Nanostructured lipid carriers
Neo-adjuvant therapy
Controlled release
dc.description.none.fl_txt_mv The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery. This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects. We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model. NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells. Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model. NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release. Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox. Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Centro de Química Inorgánica
description The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery. This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects. We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model. NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells. Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model. NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release. Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox. Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/96219
url http://sedici.unlp.edu.ar/handle/10915/96219
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/91815
info:eu-repo/semantics/altIdentifier/issn/0927-7765
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2018.06.056
info:eu-repo/semantics/altIdentifier/hdl/11336/91815
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
596-608
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.993085

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