Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica

Autores
Hiriart, Yanina; Serradell, María de los Ángeles; Martínez, Araci; Sampaolesi, Sofía; González Maciel, María Dolores; Chabalgoity, José Alejandro; Yim, Lucía; Algorta, Gabriela; Rumbo, Martín
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
Laboratorio de Investigaciones del Sistema Inmune
Facultad de Ciencias Exactas
Materia
Salud
Flagellin
Monoclonal antibodies
Salmonella
Serotyping
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85269
Acceder
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network_name_str SEDICI (UNLP)
spelling Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella entericaHiriart, YaninaSerradell, María de los ÁngelesMartínez, AraciSampaolesi, SofíaGonzález Maciel, María DoloresChabalgoity, José AlejandroYim, LucíaAlgorta, GabrielaRumbo, MartínSaludFlagellinMonoclonal antibodiesSalmonellaSerotypingIn developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.Laboratorio de Investigaciones del Sistema InmuneFacultad de Ciencias Exactas2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-9http://sedici.unlp.edu.ar/handle/10915/85269enginfo:eu-repo/semantics/altIdentifier/issn/2193-1801info:eu-repo/semantics/altIdentifier/doi/10.1186/2193-1801-2-640info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar/Creative Commons Attribution 2.5 Argentina (CC BY 2.5)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:29Zoai:sedici.unlp.edu.ar:10915/85269Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:30.052SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
spellingShingle Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
Hiriart, Yanina
Salud
Flagellin
Monoclonal antibodies
Salmonella
Serotyping
title_short Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_full Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_fullStr Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_full_unstemmed Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_sort Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
dc.creator.none.fl_str_mv Hiriart, Yanina
Serradell, María de los Ángeles
Martínez, Araci
Sampaolesi, Sofía
González Maciel, María Dolores
Chabalgoity, José Alejandro
Yim, Lucía
Algorta, Gabriela
Rumbo, Martín
author Hiriart, Yanina
author_facet Hiriart, Yanina
Serradell, María de los Ángeles
Martínez, Araci
Sampaolesi, Sofía
González Maciel, María Dolores
Chabalgoity, José Alejandro
Yim, Lucía
Algorta, Gabriela
Rumbo, Martín
author_role author
author2 Serradell, María de los Ángeles
Martínez, Araci
Sampaolesi, Sofía
González Maciel, María Dolores
Chabalgoity, José Alejandro
Yim, Lucía
Algorta, Gabriela
Rumbo, Martín
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Salud
Flagellin
Monoclonal antibodies
Salmonella
Serotyping
topic Salud
Flagellin
Monoclonal antibodies
Salmonella
Serotyping
dc.description.none.fl_txt_mv In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
Laboratorio de Investigaciones del Sistema Inmune
Facultad de Ciencias Exactas
description In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85269
url http://sedici.unlp.edu.ar/handle/10915/85269
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2193-1801
info:eu-repo/semantics/altIdentifier/doi/10.1186/2193-1801-2-640
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar/
Creative Commons Attribution 2.5 Argentina (CC BY 2.5)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar/
Creative Commons Attribution 2.5 Argentina (CC BY 2.5)
dc.format.none.fl_str_mv application/pdf
1-9
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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