Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
- Autores
- Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; Chabalgoity, Jose Alejandro; Yim, Lucia; Algorta, Gabriela; Rumbo, Martín
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
Fil: Hiriart, Yanina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Serradell, Maria de Los Angeles. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Araci. Universidad de la República; Uruguay
Fil: Sampaolesi, Sofia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez Maciel, Maria Dolores. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabalgoity, Jose Alejandro. Universidad de la República; Uruguay
Fil: Yim, Lucia. Universidad de la República; Uruguay
Fil: Algorta, Gabriela. Universidad de la República; Uruguay
Fil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
SALMONELLA
SEROTYPING
FLAGELLIN
MONOCLONAL ANTIBODIES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24379
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/24379 |
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Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella entericaHiriart, YaninaSerradell, Maria de Los AngelesMartinez, AraciSampaolesi, SofiaGonzalez Maciel, Maria DoloresChabalgoity, Jose AlejandroYim, LuciaAlgorta, GabrielaRumbo, MartínSALMONELLASEROTYPINGFLAGELLINMONOCLONAL ANTIBODIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.Fil: Hiriart, Yanina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Serradell, Maria de Los Angeles. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Araci. Universidad de la República; UruguayFil: Sampaolesi, Sofia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez Maciel, Maria Dolores. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chabalgoity, Jose Alejandro. Universidad de la República; UruguayFil: Yim, Lucia. Universidad de la República; UruguayFil: Algorta, Gabriela. Universidad de la República; UruguayFil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer Plus2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24379Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; et al.; Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica; Springer Plus; Springer Plus; 2; 640; 11-2013; 1-92193-1801CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://springerplus.springeropen.com/articles/10.1186/2193-1801-2-640info:eu-repo/semantics/altIdentifier/doi/10.1186/2193-1801-2-640info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:29Zoai:ri.conicet.gov.ar:11336/24379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:30.28CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
title |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
spellingShingle |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica Hiriart, Yanina SALMONELLA SEROTYPING FLAGELLIN MONOCLONAL ANTIBODIES |
title_short |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
title_full |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
title_fullStr |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
title_full_unstemmed |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
title_sort |
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica |
dc.creator.none.fl_str_mv |
Hiriart, Yanina Serradell, Maria de Los Angeles Martinez, Araci Sampaolesi, Sofia Gonzalez Maciel, Maria Dolores Chabalgoity, Jose Alejandro Yim, Lucia Algorta, Gabriela Rumbo, Martín |
author |
Hiriart, Yanina |
author_facet |
Hiriart, Yanina Serradell, Maria de Los Angeles Martinez, Araci Sampaolesi, Sofia Gonzalez Maciel, Maria Dolores Chabalgoity, Jose Alejandro Yim, Lucia Algorta, Gabriela Rumbo, Martín |
author_role |
author |
author2 |
Serradell, Maria de Los Angeles Martinez, Araci Sampaolesi, Sofia Gonzalez Maciel, Maria Dolores Chabalgoity, Jose Alejandro Yim, Lucia Algorta, Gabriela Rumbo, Martín |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
SALMONELLA SEROTYPING FLAGELLIN MONOCLONAL ANTIBODIES |
topic |
SALMONELLA SEROTYPING FLAGELLIN MONOCLONAL ANTIBODIES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health. Fil: Hiriart, Yanina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Serradell, Maria de Los Angeles. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Araci. Universidad de la República; Uruguay Fil: Sampaolesi, Sofia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez Maciel, Maria Dolores. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chabalgoity, Jose Alejandro. Universidad de la República; Uruguay Fil: Yim, Lucia. Universidad de la República; Uruguay Fil: Algorta, Gabriela. Universidad de la República; Uruguay Fil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/24379 Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; et al.; Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica; Springer Plus; Springer Plus; 2; 640; 11-2013; 1-9 2193-1801 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/24379 |
identifier_str_mv |
Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; et al.; Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica; Springer Plus; Springer Plus; 2; 640; 11-2013; 1-9 2193-1801 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://springerplus.springeropen.com/articles/10.1186/2193-1801-2-640 info:eu-repo/semantics/altIdentifier/doi/10.1186/2193-1801-2-640 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer Plus |
publisher.none.fl_str_mv |
Springer Plus |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269405823631360 |
score |
13.13397 |