Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica

Autores
Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; Chabalgoity, Jose Alejandro; Yim, Lucia; Algorta, Gabriela; Rumbo, Martín
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
Fil: Hiriart, Yanina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Serradell, Maria de Los Angeles. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Araci. Universidad de la República; Uruguay
Fil: Sampaolesi, Sofia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez Maciel, Maria Dolores. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabalgoity, Jose Alejandro. Universidad de la República; Uruguay
Fil: Yim, Lucia. Universidad de la República; Uruguay
Fil: Algorta, Gabriela. Universidad de la República; Uruguay
Fil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
SALMONELLA
SEROTYPING
FLAGELLIN
MONOCLONAL ANTIBODIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24379

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella entericaHiriart, YaninaSerradell, Maria de Los AngelesMartinez, AraciSampaolesi, SofiaGonzalez Maciel, Maria DoloresChabalgoity, Jose AlejandroYim, LuciaAlgorta, GabrielaRumbo, MartínSALMONELLASEROTYPINGFLAGELLINMONOCLONAL ANTIBODIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.Fil: Hiriart, Yanina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Serradell, Maria de Los Angeles. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Araci. Universidad de la República; UruguayFil: Sampaolesi, Sofia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez Maciel, Maria Dolores. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chabalgoity, Jose Alejandro. Universidad de la República; UruguayFil: Yim, Lucia. Universidad de la República; UruguayFil: Algorta, Gabriela. Universidad de la República; UruguayFil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer Plus2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24379Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; et al.; Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica; Springer Plus; Springer Plus; 2; 640; 11-2013; 1-92193-1801CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://springerplus.springeropen.com/articles/10.1186/2193-1801-2-640info:eu-repo/semantics/altIdentifier/doi/10.1186/2193-1801-2-640info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:29Zoai:ri.conicet.gov.ar:11336/24379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:30.28CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
spellingShingle Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
Hiriart, Yanina
SALMONELLA
SEROTYPING
FLAGELLIN
MONOCLONAL ANTIBODIES
title_short Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_full Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_fullStr Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_full_unstemmed Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_sort Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
dc.creator.none.fl_str_mv Hiriart, Yanina
Serradell, Maria de Los Angeles
Martinez, Araci
Sampaolesi, Sofia
Gonzalez Maciel, Maria Dolores
Chabalgoity, Jose Alejandro
Yim, Lucia
Algorta, Gabriela
Rumbo, Martín
author Hiriart, Yanina
author_facet Hiriart, Yanina
Serradell, Maria de Los Angeles
Martinez, Araci
Sampaolesi, Sofia
Gonzalez Maciel, Maria Dolores
Chabalgoity, Jose Alejandro
Yim, Lucia
Algorta, Gabriela
Rumbo, Martín
author_role author
author2 Serradell, Maria de Los Angeles
Martinez, Araci
Sampaolesi, Sofia
Gonzalez Maciel, Maria Dolores
Chabalgoity, Jose Alejandro
Yim, Lucia
Algorta, Gabriela
Rumbo, Martín
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SALMONELLA
SEROTYPING
FLAGELLIN
MONOCLONAL ANTIBODIES
topic SALMONELLA
SEROTYPING
FLAGELLIN
MONOCLONAL ANTIBODIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
Fil: Hiriart, Yanina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Serradell, Maria de Los Angeles. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Araci. Universidad de la República; Uruguay
Fil: Sampaolesi, Sofia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez Maciel, Maria Dolores. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabalgoity, Jose Alejandro. Universidad de la República; Uruguay
Fil: Yim, Lucia. Universidad de la República; Uruguay
Fil: Algorta, Gabriela. Universidad de la República; Uruguay
Fil: Rumbo, Martín. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24379
Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; et al.; Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica; Springer Plus; Springer Plus; 2; 640; 11-2013; 1-9
2193-1801
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24379
identifier_str_mv Hiriart, Yanina; Serradell, Maria de Los Angeles; Martinez, Araci; Sampaolesi, Sofia; Gonzalez Maciel, Maria Dolores; et al.; Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica; Springer Plus; Springer Plus; 2; 640; 11-2013; 1-9
2193-1801
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/2193-1801-2-640
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application/pdf
dc.publisher.none.fl_str_mv Springer Plus
publisher.none.fl_str_mv Springer Plus
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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