Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis

Autores
González, Ester Teresa; Licursi, María; Bonzo, Estela
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's J coefficients, obtaining values classified as good for both coefficients. Comparing Leucokit-La Plata and another commercial reference kit (Chekit Leucotest Bommeli AG, Bern Switzerland), the sensitivity (97.05%), specificity (94.11%), negative predictive value (92.30%) and positive predictive value (97.77%), were obtained. Applying Kappa and Jouden's Index (J) coefficients an almost perfect concordance was obtained between both kits. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/36725

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spelling Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosisGonzález, Ester TeresaLicursi, MaríaBonzo, EstelaCiencias Veterinariasbovine leukemiaBosBovinaeELISA evaluationbovine leukemia virusBovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's J coefficients, obtaining values classified as good for both coefficients. Comparing Leucokit-La Plata and another commercial reference kit (Chekit Leucotest Bommeli AG, Bern Switzerland), the sensitivity (97.05%), specificity (94.11%), negative predictive value (92.30%) and positive predictive value (97.77%), were obtained. Applying Kappa and Jouden's Index (J) coefficients an almost perfect concordance was obtained between both kits. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.Facultad de Ciencias Veterinarias2007-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-5http://sedici.unlp.edu.ar/handle/10915/36725enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/pdf/bjm/v38n1/arq01.pdfinfo:eu-repo/semantics/altIdentifier/issn/1517-8382info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T10:49:37Zoai:sedici.unlp.edu.ar:10915/36725Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 10:49:37.326SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
spellingShingle Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
González, Ester Teresa
Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
title_short Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_full Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_fullStr Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_full_unstemmed Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_sort Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
dc.creator.none.fl_str_mv González, Ester Teresa
Licursi, María
Bonzo, Estela
author González, Ester Teresa
author_facet González, Ester Teresa
Licursi, María
Bonzo, Estela
author_role author
author2 Licursi, María
Bonzo, Estela
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
topic Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
dc.description.none.fl_txt_mv Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's J coefficients, obtaining values classified as good for both coefficients. Comparing Leucokit-La Plata and another commercial reference kit (Chekit Leucotest Bommeli AG, Bern Switzerland), the sensitivity (97.05%), specificity (94.11%), negative predictive value (92.30%) and positive predictive value (97.77%), were obtained. Applying Kappa and Jouden's Index (J) coefficients an almost perfect concordance was obtained between both kits. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.
Facultad de Ciencias Veterinarias
description Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's J coefficients, obtaining values classified as good for both coefficients. Comparing Leucokit-La Plata and another commercial reference kit (Chekit Leucotest Bommeli AG, Bern Switzerland), the sensitivity (97.05%), specificity (94.11%), negative predictive value (92.30%) and positive predictive value (97.77%), were obtained. Applying Kappa and Jouden's Index (J) coefficients an almost perfect concordance was obtained between both kits. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.
publishDate 2007
dc.date.none.fl_str_mv 2007-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
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dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/1517-8382
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/
Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/3.0/
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dc.format.none.fl_str_mv application/pdf
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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