Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole

Autores
Bárcena, Alejandra; Petroselli, Gabriela; Velasquez, Silvia M.; Estevez, José M.; Erra-Balsells, Rosa; Balatti, Pedro Alberto; Saparrat, Mario Carlos Nazareno
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudocercospora griseola, an anamorph of Mycosphaerella, causes Angular Leaf Spot (ALS). The mycelia and conidia from P. griseola are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in P. griseola f. mesoamericana isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected P. griseola f. mesoamericana in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS.
Facultad de Ciencias Naturales y Museo
Instituto de Fisiología Vegetal
Centro de Investigaciones en Fitopatología
Instituto de Botánica "Dr. Carlos Spegazzini"
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Naturales
Pseudocercospora griseola f. mesoamericana
Phaseolus vulgaris
Melanin
Tricyclazole
UV-MALDI-TOFMS
Microscopy
FTIR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/130754
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to TricyclazoleBárcena, AlejandraPetroselli, GabrielaVelasquez, Silvia M.Estevez, José M.Erra-Balsells, RosaBalatti, Pedro AlbertoSaparrat, Mario Carlos NazarenoCiencias NaturalesPseudocercospora griseola f. mesoamericanaPhaseolus vulgarisMelaninTricyclazoleUV-MALDI-TOFMSMicroscopyFTIR<i>Pseudocercospora griseola</i>, an anamorph of <i>Mycosphaerella</i>, causes Angular Leaf Spot (ALS). The mycelia and conidia from <i>P. griseola</i> are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in <i>P. griseola f. mesoamericana</i> isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected <i>P. griseola f. mesoamericana</i> in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS.Facultad de Ciencias Naturales y MuseoInstituto de Fisiología VegetalCentro de Investigaciones en FitopatologíaInstituto de Botánica "Dr. Carlos Spegazzini"Facultad de Ciencias Agrarias y Forestales2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/130754enginfo:eu-repo/semantics/altIdentifier/issn/1617-416Xinfo:eu-repo/semantics/altIdentifier/issn/1861-8952info:eu-repo/semantics/altIdentifier/doi/10.1007/s11557-015-1102-7info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:37Zoai:sedici.unlp.edu.ar:10915/130754Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:38.066SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
spellingShingle Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
Bárcena, Alejandra
Ciencias Naturales
Pseudocercospora griseola f. mesoamericana
Phaseolus vulgaris
Melanin
Tricyclazole
UV-MALDI-TOFMS
Microscopy
FTIR
title_short Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_full Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_fullStr Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_full_unstemmed Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
title_sort Response of the fungus <i>Pseudocercospora griseola f. mesoamericana</i> to Tricyclazole
dc.creator.none.fl_str_mv Bárcena, Alejandra
Petroselli, Gabriela
Velasquez, Silvia M.
Estevez, José M.
Erra-Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author Bárcena, Alejandra
author_facet Bárcena, Alejandra
Petroselli, Gabriela
Velasquez, Silvia M.
Estevez, José M.
Erra-Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author_role author
author2 Petroselli, Gabriela
Velasquez, Silvia M.
Estevez, José M.
Erra-Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Pseudocercospora griseola f. mesoamericana
Phaseolus vulgaris
Melanin
Tricyclazole
UV-MALDI-TOFMS
Microscopy
FTIR
topic Ciencias Naturales
Pseudocercospora griseola f. mesoamericana
Phaseolus vulgaris
Melanin
Tricyclazole
UV-MALDI-TOFMS
Microscopy
FTIR
dc.description.none.fl_txt_mv <i>Pseudocercospora griseola</i>, an anamorph of <i>Mycosphaerella</i>, causes Angular Leaf Spot (ALS). The mycelia and conidia from <i>P. griseola</i> are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in <i>P. griseola f. mesoamericana</i> isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected <i>P. griseola f. mesoamericana</i> in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS.
Facultad de Ciencias Naturales y Museo
Instituto de Fisiología Vegetal
Centro de Investigaciones en Fitopatología
Instituto de Botánica "Dr. Carlos Spegazzini"
Facultad de Ciencias Agrarias y Forestales
description <i>Pseudocercospora griseola</i>, an anamorph of <i>Mycosphaerella</i>, causes Angular Leaf Spot (ALS). The mycelia and conidia from <i>P. griseola</i> are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in <i>P. griseola f. mesoamericana</i> isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected <i>P. griseola f. mesoamericana</i> in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/130754
url http://sedici.unlp.edu.ar/handle/10915/130754
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1617-416X
info:eu-repo/semantics/altIdentifier/issn/1861-8952
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11557-015-1102-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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