Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils

Autores
Rosenberg, Carolina Elena; Fink, Nilda Esther; Arrieta, Marcos A.; Salibian, Alfredo
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lead is an element of risk for the environment and human health and has harmful effects that may exceed those of other inorganic toxicants. The immune system is one ofthe targets of lead. Its immunomodulatory actions depend on the level of exposure, and it has been demonstrated that environmental amounts of the metal alter immune function. Very little information is available regarding the effect of the metal on different aspects of the immune system of lower vertebrates, in particular of amphibians. The aim of this study was to investigate the effect of sublethal lead (as acetate) on the function of polymorphonuclear cells of Bufo arenarum. The results revealed that phagocytic and lytic functions ofthe adherent blood cells collected from sublethal lead-injected toads and incubated with suspensions of Candida pseudotropicalis were affected negatively. The decrease of the phagocytic activity was correlated with increased blood lead levels (P-0.0001). Additional information referred to the total and differential leukocyte counts was presented; the only difference found was in the number of blast-like cells that resulted augmented in the samples of lead-injected toads. It was concluded that the evaluation ofthese parameters might be a reliable tool for the biological monitoring of the immune status of amphibians.
Facultad de Ciencias Exactas
Comisión de Investigaciones Científicas de la provincia de Buenos Aires
Materia
Biología
Lead
Phagocytosis
Lytic activity
Candicidal activity
Polymorphonuclear cells
Neutrophils
Candida pseudotropicalis
Amphibia
Bufo arenarum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/114716
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/114716
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophilsRosenberg, Carolina ElenaFink, Nilda EstherArrieta, Marcos A.Salibian, AlfredoBiologíaLeadPhagocytosisLytic activityCandicidal activityPolymorphonuclear cellsNeutrophilsCandida pseudotropicalisAmphibiaBufo arenarumLead is an element of risk for the environment and human health and has harmful effects that may exceed those of other inorganic toxicants. The immune system is one ofthe targets of lead. Its immunomodulatory actions depend on the level of exposure, and it has been demonstrated that environmental amounts of the metal alter immune function. Very little information is available regarding the effect of the metal on different aspects of the immune system of lower vertebrates, in particular of amphibians. The aim of this study was to investigate the effect of sublethal lead (as acetate) on the function of polymorphonuclear cells of Bufo arenarum. The results revealed that phagocytic and lytic functions ofthe adherent blood cells collected from sublethal lead-injected toads and incubated with suspensions of Candida pseudotropicalis were affected negatively. The decrease of the phagocytic activity was correlated with increased blood lead levels (P-0.0001). Additional information referred to the total and differential leukocyte counts was presented; the only difference found was in the number of blast-like cells that resulted augmented in the samples of lead-injected toads. It was concluded that the evaluation ofthese parameters might be a reliable tool for the biological monitoring of the immune status of amphibians.Facultad de Ciencias ExactasComisión de Investigaciones Científicas de la provincia de Buenos Aires2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf225-233http://sedici.unlp.edu.ar/handle/10915/114716enginfo:eu-repo/semantics/altIdentifier/issn/1532-0456info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cca.2003.09.004info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:26:44Zoai:sedici.unlp.edu.ar:10915/114716Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:26:44.684SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
title Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
spellingShingle Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
Rosenberg, Carolina Elena
Biología
Lead
Phagocytosis
Lytic activity
Candicidal activity
Polymorphonuclear cells
Neutrophils
Candida pseudotropicalis
Amphibia
Bufo arenarum
title_short Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
title_full Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
title_fullStr Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
title_full_unstemmed Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
title_sort Effect of lead acetate on the in vitro engulfment and killing capability of toad (<i>Bufo arenarum</i>) neutrophils
dc.creator.none.fl_str_mv Rosenberg, Carolina Elena
Fink, Nilda Esther
Arrieta, Marcos A.
Salibian, Alfredo
author Rosenberg, Carolina Elena
author_facet Rosenberg, Carolina Elena
Fink, Nilda Esther
Arrieta, Marcos A.
Salibian, Alfredo
author_role author
author2 Fink, Nilda Esther
Arrieta, Marcos A.
Salibian, Alfredo
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Lead
Phagocytosis
Lytic activity
Candicidal activity
Polymorphonuclear cells
Neutrophils
Candida pseudotropicalis
Amphibia
Bufo arenarum
topic Biología
Lead
Phagocytosis
Lytic activity
Candicidal activity
Polymorphonuclear cells
Neutrophils
Candida pseudotropicalis
Amphibia
Bufo arenarum
dc.description.none.fl_txt_mv Lead is an element of risk for the environment and human health and has harmful effects that may exceed those of other inorganic toxicants. The immune system is one ofthe targets of lead. Its immunomodulatory actions depend on the level of exposure, and it has been demonstrated that environmental amounts of the metal alter immune function. Very little information is available regarding the effect of the metal on different aspects of the immune system of lower vertebrates, in particular of amphibians. The aim of this study was to investigate the effect of sublethal lead (as acetate) on the function of polymorphonuclear cells of Bufo arenarum. The results revealed that phagocytic and lytic functions ofthe adherent blood cells collected from sublethal lead-injected toads and incubated with suspensions of Candida pseudotropicalis were affected negatively. The decrease of the phagocytic activity was correlated with increased blood lead levels (P-0.0001). Additional information referred to the total and differential leukocyte counts was presented; the only difference found was in the number of blast-like cells that resulted augmented in the samples of lead-injected toads. It was concluded that the evaluation ofthese parameters might be a reliable tool for the biological monitoring of the immune status of amphibians.
Facultad de Ciencias Exactas
Comisión de Investigaciones Científicas de la provincia de Buenos Aires
description Lead is an element of risk for the environment and human health and has harmful effects that may exceed those of other inorganic toxicants. The immune system is one ofthe targets of lead. Its immunomodulatory actions depend on the level of exposure, and it has been demonstrated that environmental amounts of the metal alter immune function. Very little information is available regarding the effect of the metal on different aspects of the immune system of lower vertebrates, in particular of amphibians. The aim of this study was to investigate the effect of sublethal lead (as acetate) on the function of polymorphonuclear cells of Bufo arenarum. The results revealed that phagocytic and lytic functions ofthe adherent blood cells collected from sublethal lead-injected toads and incubated with suspensions of Candida pseudotropicalis were affected negatively. The decrease of the phagocytic activity was correlated with increased blood lead levels (P-0.0001). Additional information referred to the total and differential leukocyte counts was presented; the only difference found was in the number of blast-like cells that resulted augmented in the samples of lead-injected toads. It was concluded that the evaluation ofthese parameters might be a reliable tool for the biological monitoring of the immune status of amphibians.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/114716
url http://sedici.unlp.edu.ar/handle/10915/114716
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1532-0456
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cca.2003.09.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
225-233
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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