Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium

Autores
López, Claudia S.; Heras, Horacio; Ruzal, Sandra M.; Sanchez-Rivas, Carmen; Rivas, Emilio A.
Año de publicación
1998
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137925
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic MediumLópez, Claudia S.Heras, HoracioRuzal, Sandra M.Sanchez-Rivas, CarmenRivas, Emilio A.BiologíaQuímicaBacillus subtilisHyperosmotic Mediumhypertonic culturesThe envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed.Instituto de Investigaciones Bioquímicas de La Plata1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf55-61http://sedici.unlp.edu.ar/handle/10915/137925enginfo:eu-repo/semantics/altIdentifier/issn/0343-8651info:eu-repo/semantics/altIdentifier/issn/1432-0991info:eu-repo/semantics/altIdentifier/doi/10.1007/s002849900279info:eu-repo/semantics/altIdentifier/pmid/9405747info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:58Zoai:sedici.unlp.edu.ar:10915/137925Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:58.422SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
spellingShingle Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
López, Claudia S.
Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
title_short Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_full Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_fullStr Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_full_unstemmed Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_sort Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
dc.creator.none.fl_str_mv López, Claudia S.
Heras, Horacio
Ruzal, Sandra M.
Sanchez-Rivas, Carmen
Rivas, Emilio A.
author López, Claudia S.
author_facet López, Claudia S.
Heras, Horacio
Ruzal, Sandra M.
Sanchez-Rivas, Carmen
Rivas, Emilio A.
author_role author
author2 Heras, Horacio
Ruzal, Sandra M.
Sanchez-Rivas, Carmen
Rivas, Emilio A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
topic Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
dc.description.none.fl_txt_mv The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed.
Instituto de Investigaciones Bioquímicas de La Plata
description The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137925
url http://sedici.unlp.edu.ar/handle/10915/137925
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0343-8651
info:eu-repo/semantics/altIdentifier/issn/1432-0991
info:eu-repo/semantics/altIdentifier/doi/10.1007/s002849900279
info:eu-repo/semantics/altIdentifier/pmid/9405747
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
55-61
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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