Efficient evaluation of a gene containment system for poplar through early flowering induction

Autores
Briones, María Valentina; Hoenicka, Hans; Cañas, Luis A.; Beltrán, José Pío; Hanelt, Dieter; Sharry, Sandra Elizabeth; Fladung, Matthias
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vertical gene transfer through pollen fow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene fow among tree species. However, evaluation of genetic containment strategies for trees is very difcult due to the long-generation times. Early fowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile fowers in poplar, recently, a new early fowering approach was developed. In this study, early fowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efciency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confrmed barnase gene activity in fowers, and pollen development was disturbed, leading to sterile fowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107399
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/107399
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Efficient evaluation of a gene containment system for poplar through early flowering inductionBriones, María ValentinaHoenicka, HansCañas, Luis A.Beltrán, José PíoHanelt, DieterSharry, Sandra ElizabethFladung, MatthiasCiencias AgrariasBotánicaGene containmentEarly foweringPopulusGenetic transformationPsEND1BiosafetyVertical gene transfer through pollen fow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene fow among tree species. However, evaluation of genetic containment strategies for trees is very difcult due to the long-generation times. Early fowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile fowers in poplar, recently, a new early fowering approach was developed. In this study, early fowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efciency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confrmed barnase gene activity in fowers, and pollen development was disturbed, leading to sterile fowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.Facultad de Ciencias Agrarias y Forestales2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf577-587http://sedici.unlp.edu.ar/handle/10915/107399enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7165154&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/1432-203Xinfo:eu-repo/semantics/altIdentifier/pmid/32052127info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-020-02515-1info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:04:47Zoai:sedici.unlp.edu.ar:10915/107399Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:04:47.352SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Efficient evaluation of a gene containment system for poplar through early flowering induction
title Efficient evaluation of a gene containment system for poplar through early flowering induction
spellingShingle Efficient evaluation of a gene containment system for poplar through early flowering induction
Briones, María Valentina
Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
title_short Efficient evaluation of a gene containment system for poplar through early flowering induction
title_full Efficient evaluation of a gene containment system for poplar through early flowering induction
title_fullStr Efficient evaluation of a gene containment system for poplar through early flowering induction
title_full_unstemmed Efficient evaluation of a gene containment system for poplar through early flowering induction
title_sort Efficient evaluation of a gene containment system for poplar through early flowering induction
dc.creator.none.fl_str_mv Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A.
Beltrán, José Pío
Hanelt, Dieter
Sharry, Sandra Elizabeth
Fladung, Matthias
author Briones, María Valentina
author_facet Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A.
Beltrán, José Pío
Hanelt, Dieter
Sharry, Sandra Elizabeth
Fladung, Matthias
author_role author
author2 Hoenicka, Hans
Cañas, Luis A.
Beltrán, José Pío
Hanelt, Dieter
Sharry, Sandra Elizabeth
Fladung, Matthias
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
topic Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
dc.description.none.fl_txt_mv Vertical gene transfer through pollen fow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene fow among tree species. However, evaluation of genetic containment strategies for trees is very difcult due to the long-generation times. Early fowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile fowers in poplar, recently, a new early fowering approach was developed. In this study, early fowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efciency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confrmed barnase gene activity in fowers, and pollen development was disturbed, leading to sterile fowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
Facultad de Ciencias Agrarias y Forestales
description Vertical gene transfer through pollen fow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene fow among tree species. However, evaluation of genetic containment strategies for trees is very difcult due to the long-generation times. Early fowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile fowers in poplar, recently, a new early fowering approach was developed. In this study, early fowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efciency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confrmed barnase gene activity in fowers, and pollen development was disturbed, leading to sterile fowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107399
url http://sedici.unlp.edu.ar/handle/10915/107399
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7165154&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/1432-203X
info:eu-repo/semantics/altIdentifier/pmid/32052127
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-020-02515-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
577-587
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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