Efficient evaluation of a gene containment system for poplar through early flowering induction

Autores
Briones, María Valentina; Hoenicka, Hans; Cañas, Luis A,; Beltrán, José Pío; Dieter, Hanelt; Sharry, Sandra Elizabeth; Fladung, Matthias
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
Fil: Briones, María Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal. Centro Exptal.de Propagación Vegetativa; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Hoenicka, Hans. Thunen Institute; Alemania
Fil: Cañas, Luis A,. Instituto de Biologia Molecular de Plantas Valencia; España
Fil: Beltrán, José Pío. Universidad de Valencia; España
Fil: Dieter, Hanelt. Thunen Institute; Alemania
Fil: Sharry, Sandra Elizabeth. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Fladung, Matthias. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal. Centro Exptal.de Propagación Vegetativa; Argentina
Materia
Gene containment
POPULUS
BIOSAFETY
TRANSGENIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107166
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Efficient evaluation of a gene containment system for poplar through early flowering inductionBriones, María ValentinaHoenicka, HansCañas, Luis A,Beltrán, José PíoDieter, HaneltSharry, Sandra ElizabethFladung, MatthiasGene containmentPOPULUSBIOSAFETYTRANSGENIChttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.Fil: Briones, María Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal. Centro Exptal.de Propagación Vegetativa; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Hoenicka, Hans. Thunen Institute; AlemaniaFil: Cañas, Luis A,. Instituto de Biologia Molecular de Plantas Valencia; EspañaFil: Beltrán, José Pío. Universidad de Valencia; EspañaFil: Dieter, Hanelt. Thunen Institute; AlemaniaFil: Sharry, Sandra Elizabeth. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Fladung, Matthias. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal. Centro Exptal.de Propagación Vegetativa; ArgentinaSpringer2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107166Briones, María Valentina; Hoenicka, Hans ; Cañas, Luis A,; Beltrán, José Pío; Dieter, Hanelt; et al.; Efficient evaluation of a gene containment system for poplar through early flowering induction; Springer; Plant Cell Reports; 2-20200721-7714CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00299-020-02515-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-020-02515-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:43:53Zoai:ri.conicet.gov.ar:11336/107166instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:43:53.528CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficient evaluation of a gene containment system for poplar through early flowering induction
title Efficient evaluation of a gene containment system for poplar through early flowering induction
spellingShingle Efficient evaluation of a gene containment system for poplar through early flowering induction
Briones, María Valentina
Gene containment
POPULUS
BIOSAFETY
TRANSGENIC
title_short Efficient evaluation of a gene containment system for poplar through early flowering induction
title_full Efficient evaluation of a gene containment system for poplar through early flowering induction
title_fullStr Efficient evaluation of a gene containment system for poplar through early flowering induction
title_full_unstemmed Efficient evaluation of a gene containment system for poplar through early flowering induction
title_sort Efficient evaluation of a gene containment system for poplar through early flowering induction
dc.creator.none.fl_str_mv Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A,
Beltrán, José Pío
Dieter, Hanelt
Sharry, Sandra Elizabeth
Fladung, Matthias
author Briones, María Valentina
author_facet Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A,
Beltrán, José Pío
Dieter, Hanelt
Sharry, Sandra Elizabeth
Fladung, Matthias
author_role author
author2 Hoenicka, Hans
Cañas, Luis A,
Beltrán, José Pío
Dieter, Hanelt
Sharry, Sandra Elizabeth
Fladung, Matthias
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Gene containment
POPULUS
BIOSAFETY
TRANSGENIC
topic Gene containment
POPULUS
BIOSAFETY
TRANSGENIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
Fil: Briones, María Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal. Centro Exptal.de Propagación Vegetativa; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Hoenicka, Hans. Thunen Institute; Alemania
Fil: Cañas, Luis A,. Instituto de Biologia Molecular de Plantas Valencia; España
Fil: Beltrán, José Pío. Universidad de Valencia; España
Fil: Dieter, Hanelt. Thunen Institute; Alemania
Fil: Sharry, Sandra Elizabeth. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Fladung, Matthias. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Tecnología Agropecuaria y Forestal. Centro Exptal.de Propagación Vegetativa; Argentina
description Vertical gene transfer through pollen flow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene flow among tree species. However, evaluation of genetic containment strategies for trees is very difficult due to the long-generation times. Early flowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile flowers in poplar, recently, a new early flowering approach was developed. In this study, early flowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efficiency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confirmed barnase gene activity in flowers, and pollen development was disturbed, leading to sterile flowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107166
Briones, María Valentina; Hoenicka, Hans ; Cañas, Luis A,; Beltrán, José Pío; Dieter, Hanelt; et al.; Efficient evaluation of a gene containment system for poplar through early flowering induction; Springer; Plant Cell Reports; 2-2020
0721-7714
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107166
identifier_str_mv Briones, María Valentina; Hoenicka, Hans ; Cañas, Luis A,; Beltrán, José Pío; Dieter, Hanelt; et al.; Efficient evaluation of a gene containment system for poplar through early flowering induction; Springer; Plant Cell Reports; 2-2020
0721-7714
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00299-020-02515-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-020-02515-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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