Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes

Autores
Carranza Martín, Ana Cristina; Parker, Anthony J.; Furnus, Cecilia Cristina; Relling, Alejandro Enrique
Año de publicación
2020
Idioma
inglés
Tipo de recurso
conjunto de datos
Estado
versión publicada
Descripción
A negative energy balance (NEB) is detrimental to reproduction in animals. A suggested link between NEB and reproductive failure is the gastrointestinal hormone ghrelin, because of the association between ghrelin and the hypothalamo-pituitary-gonadal axis. The [D-Lys3]-Growth Hormone Releasing Peptide-6 ([D-Lys3]-GHRP-6) is a ghrelin antagonist that acts on ghrelin receptors (GHS-R1). The objective of this study was to evaluate the effect of [D-Lys3]-GHRP-6 on reproduction variables in feed restricted ewes. Two experiments were conducted. Experiment I was conducted for 30 days; and Experiment II for 13 days. In both experiments the ewes (n = 18) were randomly assigned to: Control (CO): fed to meet maintenance requirements; Feed restriction (FR): 80% of maintenance restriction; or Ghrelin antagonist (GA): feed restricted and daily subcutaneous of 7.5μg/kg of [D-Lys3]-GHRP-6. Plasma was collected to measure hormones and metabolite concentration. In Experiment II, the hypothalamus and ovaries were collected on day 13. In both Experiments, sheep allocated to the FR and GA treatments decreased their body weight compared with sheep in the CO group (P 0.10). Experiment I: Plasma ghrelin concentration was greater (P < 0.01) in FR and GA compared with CO ewes. Plasma non-esterified fatty acids concentration was greater (P < 0.01) in GA and FR than CO. Experiment II: Kisspeptin1-Receptor (Kiss1-R) mRNA expression was greater in FR (P < 0.01) and tended to be greater in GA (P = 0.10) compared with CO ewes. The neuro peptide-Y (NPY) mRNA expression was greater (P = 0.03) in FR than CO; and tended to be greater (P = 0.06) compared with GA ewes. Growth hormone releasing hormone (GhRH) mRNA expression was greater in GA (P = 0.04) and tended to be greater in FR (P = 0.07) compared with CO ewes. Feed restriction increased GhRH, NPY, and Kiss-R mRNA expression in hypothalamus without affecting reproductive variables.Ghrelin antagonist may prevent an increase inNPY expression in ewes.
Fil: Carranza Martín, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.
Fil: Furnus, Cecilia Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.
Instituto de Genética Veterinaria
Materia
Ciencias Veterinarias
http://voc.sedici.unlp.edu.ar/taxonomy/term/398206
Gherlin
Ovaries
Diet
Progesterone
Hormones
Hypothalamus
Blood plasma
Body weight
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/125956

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network_name_str SEDICI (UNLP)
spelling Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewesCarranza Martín, Ana CristinaParker, Anthony J.Furnus, Cecilia CristinaRelling, Alejandro EnriqueCiencias Veterinariashttp://voc.sedici.unlp.edu.ar/taxonomy/term/398206GherlinOvariesDietProgesteroneHormonesHypothalamusBlood plasmaBody weightA negative energy balance (NEB) is detrimental to reproduction in animals. A suggested link between NEB and reproductive failure is the gastrointestinal hormone ghrelin, because of the association between ghrelin and the hypothalamo-pituitary-gonadal axis. The [D-Lys3]-Growth Hormone Releasing Peptide-6 ([D-Lys3]-GHRP-6) is a ghrelin antagonist that acts on ghrelin receptors (GHS-R1). The objective of this study was to evaluate the effect of [D-Lys3]-GHRP-6 on reproduction variables in feed restricted ewes. Two experiments were conducted. Experiment I was conducted for 30 days; and Experiment II for 13 days. In both experiments the ewes (n = 18) were randomly assigned to: Control (CO): fed to meet maintenance requirements; Feed restriction (FR): 80% of maintenance restriction; or Ghrelin antagonist (GA): feed restricted and daily subcutaneous of 7.5μg/kg of [D-Lys3]-GHRP-6. Plasma was collected to measure hormones and metabolite concentration. In Experiment II, the hypothalamus and ovaries were collected on day 13. In both Experiments, sheep allocated to the FR and GA treatments decreased their body weight compared with sheep in the CO group (P 0.10). Experiment I: Plasma ghrelin concentration was greater (P < 0.01) in FR and GA compared with CO ewes. Plasma non-esterified fatty acids concentration was greater (P < 0.01) in GA and FR than CO. Experiment II: Kisspeptin1-Receptor (Kiss1-R) mRNA expression was greater in FR (P < 0.01) and tended to be greater in GA (P = 0.10) compared with CO ewes. The neuro peptide-Y (NPY) mRNA expression was greater (P = 0.03) in FR than CO; and tended to be greater (P = 0.06) compared with GA ewes. Growth hormone releasing hormone (GhRH) mRNA expression was greater in GA (P = 0.04) and tended to be greater in FR (P = 0.07) compared with CO ewes. Feed restriction increased GhRH, NPY, and Kiss-R mRNA expression in hypothalamus without affecting reproductive variables.Ghrelin antagonist may prevent an increase inNPY expression in ewes.Fil: Carranza Martín, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.Fil: Furnus, Cecilia Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.Instituto de Genética Veterinaria2020-09-09info:eu-repo/semantics/publishedVersionConjunto de datoshttp://purl.org/coar/resource_type/c_ddb1info:ar-repo/semantics/conjuntoDeDatosinfo:eu-repo/semantics/dataSetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://sedici.unlp.edu.ar/handle/10915/125956enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0238465.s001info:eu-repo/semantics/reference/hdl/10915/125952info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:23Zoai:sedici.unlp.edu.ar:10915/125956Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:24.271SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
title Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
spellingShingle Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
Carranza Martín, Ana Cristina
Ciencias Veterinarias
http://voc.sedici.unlp.edu.ar/taxonomy/term/398206
Gherlin
Ovaries
Diet
Progesterone
Hormones
Hypothalamus
Blood plasma
Body weight
title_short Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
title_full Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
title_fullStr Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
title_full_unstemmed Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
title_sort Dataset: Ghrelin antagonist overrides the mRNA expression of NPY in hypothalamus in feed restricted ewes
dc.creator.none.fl_str_mv Carranza Martín, Ana Cristina
Parker, Anthony J.
Furnus, Cecilia Cristina
Relling, Alejandro Enrique
author Carranza Martín, Ana Cristina
author_facet Carranza Martín, Ana Cristina
Parker, Anthony J.
Furnus, Cecilia Cristina
Relling, Alejandro Enrique
author_role author
author2 Parker, Anthony J.
Furnus, Cecilia Cristina
Relling, Alejandro Enrique
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
http://voc.sedici.unlp.edu.ar/taxonomy/term/398206
Gherlin
Ovaries
Diet
Progesterone
Hormones
Hypothalamus
Blood plasma
Body weight
topic Ciencias Veterinarias
http://voc.sedici.unlp.edu.ar/taxonomy/term/398206
Gherlin
Ovaries
Diet
Progesterone
Hormones
Hypothalamus
Blood plasma
Body weight
dc.description.none.fl_txt_mv A negative energy balance (NEB) is detrimental to reproduction in animals. A suggested link between NEB and reproductive failure is the gastrointestinal hormone ghrelin, because of the association between ghrelin and the hypothalamo-pituitary-gonadal axis. The [D-Lys3]-Growth Hormone Releasing Peptide-6 ([D-Lys3]-GHRP-6) is a ghrelin antagonist that acts on ghrelin receptors (GHS-R1). The objective of this study was to evaluate the effect of [D-Lys3]-GHRP-6 on reproduction variables in feed restricted ewes. Two experiments were conducted. Experiment I was conducted for 30 days; and Experiment II for 13 days. In both experiments the ewes (n = 18) were randomly assigned to: Control (CO): fed to meet maintenance requirements; Feed restriction (FR): 80% of maintenance restriction; or Ghrelin antagonist (GA): feed restricted and daily subcutaneous of 7.5μg/kg of [D-Lys3]-GHRP-6. Plasma was collected to measure hormones and metabolite concentration. In Experiment II, the hypothalamus and ovaries were collected on day 13. In both Experiments, sheep allocated to the FR and GA treatments decreased their body weight compared with sheep in the CO group (P 0.10). Experiment I: Plasma ghrelin concentration was greater (P < 0.01) in FR and GA compared with CO ewes. Plasma non-esterified fatty acids concentration was greater (P < 0.01) in GA and FR than CO. Experiment II: Kisspeptin1-Receptor (Kiss1-R) mRNA expression was greater in FR (P < 0.01) and tended to be greater in GA (P = 0.10) compared with CO ewes. The neuro peptide-Y (NPY) mRNA expression was greater (P = 0.03) in FR than CO; and tended to be greater (P = 0.06) compared with GA ewes. Growth hormone releasing hormone (GhRH) mRNA expression was greater in GA (P = 0.04) and tended to be greater in FR (P = 0.07) compared with CO ewes. Feed restriction increased GhRH, NPY, and Kiss-R mRNA expression in hypothalamus without affecting reproductive variables.Ghrelin antagonist may prevent an increase inNPY expression in ewes.
Fil: Carranza Martín, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.
Fil: Furnus, Cecilia Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina.
Instituto de Genética Veterinaria
description A negative energy balance (NEB) is detrimental to reproduction in animals. A suggested link between NEB and reproductive failure is the gastrointestinal hormone ghrelin, because of the association between ghrelin and the hypothalamo-pituitary-gonadal axis. The [D-Lys3]-Growth Hormone Releasing Peptide-6 ([D-Lys3]-GHRP-6) is a ghrelin antagonist that acts on ghrelin receptors (GHS-R1). The objective of this study was to evaluate the effect of [D-Lys3]-GHRP-6 on reproduction variables in feed restricted ewes. Two experiments were conducted. Experiment I was conducted for 30 days; and Experiment II for 13 days. In both experiments the ewes (n = 18) were randomly assigned to: Control (CO): fed to meet maintenance requirements; Feed restriction (FR): 80% of maintenance restriction; or Ghrelin antagonist (GA): feed restricted and daily subcutaneous of 7.5μg/kg of [D-Lys3]-GHRP-6. Plasma was collected to measure hormones and metabolite concentration. In Experiment II, the hypothalamus and ovaries were collected on day 13. In both Experiments, sheep allocated to the FR and GA treatments decreased their body weight compared with sheep in the CO group (P 0.10). Experiment I: Plasma ghrelin concentration was greater (P < 0.01) in FR and GA compared with CO ewes. Plasma non-esterified fatty acids concentration was greater (P < 0.01) in GA and FR than CO. Experiment II: Kisspeptin1-Receptor (Kiss1-R) mRNA expression was greater in FR (P < 0.01) and tended to be greater in GA (P = 0.10) compared with CO ewes. The neuro peptide-Y (NPY) mRNA expression was greater (P = 0.03) in FR than CO; and tended to be greater (P = 0.06) compared with GA ewes. Growth hormone releasing hormone (GhRH) mRNA expression was greater in GA (P = 0.04) and tended to be greater in FR (P = 0.07) compared with CO ewes. Feed restriction increased GhRH, NPY, and Kiss-R mRNA expression in hypothalamus without affecting reproductive variables.Ghrelin antagonist may prevent an increase inNPY expression in ewes.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-09
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Conjunto de datos
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