Hypophysotropic activity of histone H3 in vitro

Autores
Brown, Oscar Alfredo; Sosa, Yolanda Elena; Naumovich, Diana; Hereñú, Claudia Beatriz; Goya, Rodolfo Gustavo
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6mMH3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/164076
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/164076
network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling Hypophysotropic activity of histone H3 in vitroBrown, Oscar AlfredoSosa, Yolanda ElenaNaumovich, DianaHereñú, Claudia BeatrizGoya, Rodolfo GustavoBioquímicaHistonesHypophysotropic activityPituitary cellsSecond messengersApoptotic signalsTo assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6mMH3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals.Instituto de Investigaciones Bioquímicas de La Plata2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf671-678http://sedici.unlp.edu.ar/handle/10915/164076enginfo:eu-repo/semantics/altIdentifier/issn/0196-9781info:eu-repo/semantics/altIdentifier/doi/10.1016/S0196-9781(03)00132-3info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-12T11:07:34Zoai:sedici.unlp.edu.ar:10915/164076Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-12 11:07:34.742SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Hypophysotropic activity of histone H3 in vitro
title Hypophysotropic activity of histone H3 in vitro
spellingShingle Hypophysotropic activity of histone H3 in vitro
Brown, Oscar Alfredo
Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
title_short Hypophysotropic activity of histone H3 in vitro
title_full Hypophysotropic activity of histone H3 in vitro
title_fullStr Hypophysotropic activity of histone H3 in vitro
title_full_unstemmed Hypophysotropic activity of histone H3 in vitro
title_sort Hypophysotropic activity of histone H3 in vitro
dc.creator.none.fl_str_mv Brown, Oscar Alfredo
Sosa, Yolanda Elena
Naumovich, Diana
Hereñú, Claudia Beatriz
Goya, Rodolfo Gustavo
author Brown, Oscar Alfredo
author_facet Brown, Oscar Alfredo
Sosa, Yolanda Elena
Naumovich, Diana
Hereñú, Claudia Beatriz
Goya, Rodolfo Gustavo
author_role author
author2 Sosa, Yolanda Elena
Naumovich, Diana
Hereñú, Claudia Beatriz
Goya, Rodolfo Gustavo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
topic Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
dc.description.none.fl_txt_mv To assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6mMH3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals.
Instituto de Investigaciones Bioquímicas de La Plata
description To assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6mMH3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/164076
url http://sedici.unlp.edu.ar/handle/10915/164076
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0196-9781
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0196-9781(03)00132-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
671-678
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.137801

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