Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant

Autores
Talbert, Paul B.; Masuelli, Ricardo Williams; Tyagi, Anand P.; Comai, Luca; Henikoff, Steven
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Centromeric H3-like histones, which replace histone H3 in the centromeric chromatin of animals and fungi, have not been reported in plants. We identified a histone H3 variant from Arabidopsis thaliana that encodes a centromere-identifying protein designated HTR12. By immunological detection, HTR12 localized at centromeres in both mitotic and meiotic cells. HTR12 signal revealed tissue- and stage-specific differences in centromere morphology, including a distended bead-like structure in interphase root tip cells. The anti-HTR12 antibody also detected spherical organelles in meiotic cells. Although the antibody does not label centromeres in the closely related species Arabidopsis arenosa, HTR12 signal was found on all centromeres in allopolyploids of these two species. Comparison of the HTR12 genes of A. thaliana and A. arenosa revealed striking adaptive evolution in the N-terminal tail of the protein, similar to the pattern seen in its counterpart in Drosophila. This finding suggests that the same evolutionary forces shape centromeric chromatin in both animals and plants.
Fil: Talbert, Paul B.. Howard Hughes Medical Institute; Estados Unidos
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Howard Hughes Medical Institute; Estados Unidos
Fil: Tyagi, Anand P.. Howard Hughes Medical Institute; Estados Unidos. University of Washington; Estados Unidos
Fil: Comai, Luca. University of Washington; Estados Unidos
Fil: Henikoff, Steven. Howard Hughes Medical Institute; Estados Unidos
Materia
Histone H3
Centromere
Arabidopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/169163
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/169163
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Centromeric localization and adaptive evolution of an arabidopsis histone H3 variantTalbert, Paul B.Masuelli, Ricardo WilliamsTyagi, Anand P.Comai, LucaHenikoff, StevenHistone H3CentromereArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Centromeric H3-like histones, which replace histone H3 in the centromeric chromatin of animals and fungi, have not been reported in plants. We identified a histone H3 variant from Arabidopsis thaliana that encodes a centromere-identifying protein designated HTR12. By immunological detection, HTR12 localized at centromeres in both mitotic and meiotic cells. HTR12 signal revealed tissue- and stage-specific differences in centromere morphology, including a distended bead-like structure in interphase root tip cells. The anti-HTR12 antibody also detected spherical organelles in meiotic cells. Although the antibody does not label centromeres in the closely related species Arabidopsis arenosa, HTR12 signal was found on all centromeres in allopolyploids of these two species. Comparison of the HTR12 genes of A. thaliana and A. arenosa revealed striking adaptive evolution in the N-terminal tail of the protein, similar to the pattern seen in its counterpart in Drosophila. This finding suggests that the same evolutionary forces shape centromeric chromatin in both animals and plants.Fil: Talbert, Paul B.. Howard Hughes Medical Institute; Estados UnidosFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Howard Hughes Medical Institute; Estados UnidosFil: Tyagi, Anand P.. Howard Hughes Medical Institute; Estados Unidos. University of Washington; Estados UnidosFil: Comai, Luca. University of Washington; Estados UnidosFil: Henikoff, Steven. Howard Hughes Medical Institute; Estados UnidosAmerican Society of Plant Biologist2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/169163Talbert, Paul B.; Masuelli, Ricardo Williams; Tyagi, Anand P.; Comai, Luca; Henikoff, Steven; Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant; American Society of Plant Biologist; Plant Cell; 14; 5; 12-2002; 1053-10661040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/14/5/1053info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.010425info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/12034896/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:03:06Zoai:ri.conicet.gov.ar:11336/169163instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:03:06.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
title Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
spellingShingle Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
Talbert, Paul B.
Histone H3
Centromere
Arabidopsis
title_short Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
title_full Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
title_fullStr Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
title_full_unstemmed Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
title_sort Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant
dc.creator.none.fl_str_mv Talbert, Paul B.
Masuelli, Ricardo Williams
Tyagi, Anand P.
Comai, Luca
Henikoff, Steven
author Talbert, Paul B.
author_facet Talbert, Paul B.
Masuelli, Ricardo Williams
Tyagi, Anand P.
Comai, Luca
Henikoff, Steven
author_role author
author2 Masuelli, Ricardo Williams
Tyagi, Anand P.
Comai, Luca
Henikoff, Steven
author2_role author
author
author
author
dc.subject.none.fl_str_mv Histone H3
Centromere
Arabidopsis
topic Histone H3
Centromere
Arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Centromeric H3-like histones, which replace histone H3 in the centromeric chromatin of animals and fungi, have not been reported in plants. We identified a histone H3 variant from Arabidopsis thaliana that encodes a centromere-identifying protein designated HTR12. By immunological detection, HTR12 localized at centromeres in both mitotic and meiotic cells. HTR12 signal revealed tissue- and stage-specific differences in centromere morphology, including a distended bead-like structure in interphase root tip cells. The anti-HTR12 antibody also detected spherical organelles in meiotic cells. Although the antibody does not label centromeres in the closely related species Arabidopsis arenosa, HTR12 signal was found on all centromeres in allopolyploids of these two species. Comparison of the HTR12 genes of A. thaliana and A. arenosa revealed striking adaptive evolution in the N-terminal tail of the protein, similar to the pattern seen in its counterpart in Drosophila. This finding suggests that the same evolutionary forces shape centromeric chromatin in both animals and plants.
Fil: Talbert, Paul B.. Howard Hughes Medical Institute; Estados Unidos
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Howard Hughes Medical Institute; Estados Unidos
Fil: Tyagi, Anand P.. Howard Hughes Medical Institute; Estados Unidos. University of Washington; Estados Unidos
Fil: Comai, Luca. University of Washington; Estados Unidos
Fil: Henikoff, Steven. Howard Hughes Medical Institute; Estados Unidos
description Centromeric H3-like histones, which replace histone H3 in the centromeric chromatin of animals and fungi, have not been reported in plants. We identified a histone H3 variant from Arabidopsis thaliana that encodes a centromere-identifying protein designated HTR12. By immunological detection, HTR12 localized at centromeres in both mitotic and meiotic cells. HTR12 signal revealed tissue- and stage-specific differences in centromere morphology, including a distended bead-like structure in interphase root tip cells. The anti-HTR12 antibody also detected spherical organelles in meiotic cells. Although the antibody does not label centromeres in the closely related species Arabidopsis arenosa, HTR12 signal was found on all centromeres in allopolyploids of these two species. Comparison of the HTR12 genes of A. thaliana and A. arenosa revealed striking adaptive evolution in the N-terminal tail of the protein, similar to the pattern seen in its counterpart in Drosophila. This finding suggests that the same evolutionary forces shape centromeric chromatin in both animals and plants.
publishDate 2002
dc.date.none.fl_str_mv 2002-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/169163
Talbert, Paul B.; Masuelli, Ricardo Williams; Tyagi, Anand P.; Comai, Luca; Henikoff, Steven; Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant; American Society of Plant Biologist; Plant Cell; 14; 5; 12-2002; 1053-1066
1040-4651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/169163
identifier_str_mv Talbert, Paul B.; Masuelli, Ricardo Williams; Tyagi, Anand P.; Comai, Luca; Henikoff, Steven; Centromeric localization and adaptive evolution of an arabidopsis histone H3 variant; American Society of Plant Biologist; Plant Cell; 14; 5; 12-2002; 1053-1066
1040-4651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/14/5/1053
info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.010425
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/12034896/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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