A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival

Autores
Cafiero, Juan Hilario; Lamberti, Yanina Andrea; Surmann, Kristin; Vecerek, Branislav; Rodríguez, María Eugenia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bordetella pertussis, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular B. pertussis we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in B. pertussis intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard in vitro growth conditions we found that BP0414 is required for B. pertussis growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in B. pertussis viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the mgtC deficient mutant strain suggesting that in intracellular B. pertussis the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in B. pertussis pathogenesis and its contribution to bacterial survival within immune cells.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Biología
Bordetella pertussis
intracellular pathogens
macrophages
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104721
Acceder
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network_name_str SEDICI (UNLP)
spelling A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survivalCafiero, Juan HilarioLamberti, Yanina AndreaSurmann, KristinVecerek, BranislavRodríguez, María EugeniaCiencias ExactasBiologíaBordetella pertussisintracellular pathogensmacrophages<i>Bordetella pertussis</i>, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular <i>B. pertussis</i> we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in <i>B. pertussis</i> intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard <i>in vitro</i> growth conditions we found that BP0414 is required for <i>B. pertussis</i> growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in <i>B. pertussis</i> viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the <i>mgtC</i> deficient mutant strain suggesting that in intracellular <i>B. pertussis</i> the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in <i>B. pertussis</i> pathogenesis and its contribution to bacterial survival within immune cells.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104721enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/93561info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203204info:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0203204info:eu-repo/semantics/altIdentifier/hdl/11336/93561info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:43Zoai:sedici.unlp.edu.ar:10915/104721Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:44.302SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
title A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
spellingShingle A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
Cafiero, Juan Hilario
Ciencias Exactas
Biología
Bordetella pertussis
intracellular pathogens
macrophages
title_short A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
title_full A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
title_fullStr A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
title_full_unstemmed A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
title_sort A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
dc.creator.none.fl_str_mv Cafiero, Juan Hilario
Lamberti, Yanina Andrea
Surmann, Kristin
Vecerek, Branislav
Rodríguez, María Eugenia
author Cafiero, Juan Hilario
author_facet Cafiero, Juan Hilario
Lamberti, Yanina Andrea
Surmann, Kristin
Vecerek, Branislav
Rodríguez, María Eugenia
author_role author
author2 Lamberti, Yanina Andrea
Surmann, Kristin
Vecerek, Branislav
Rodríguez, María Eugenia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Bordetella pertussis
intracellular pathogens
macrophages
topic Ciencias Exactas
Biología
Bordetella pertussis
intracellular pathogens
macrophages
dc.description.none.fl_txt_mv <i>Bordetella pertussis</i>, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular <i>B. pertussis</i> we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in <i>B. pertussis</i> intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard <i>in vitro</i> growth conditions we found that BP0414 is required for <i>B. pertussis</i> growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in <i>B. pertussis</i> viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the <i>mgtC</i> deficient mutant strain suggesting that in intracellular <i>B. pertussis</i> the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in <i>B. pertussis</i> pathogenesis and its contribution to bacterial survival within immune cells.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description <i>Bordetella pertussis</i>, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular <i>B. pertussis</i> we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in <i>B. pertussis</i> intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard <i>in vitro</i> growth conditions we found that BP0414 is required for <i>B. pertussis</i> growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in <i>B. pertussis</i> viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the <i>mgtC</i> deficient mutant strain suggesting that in intracellular <i>B. pertussis</i> the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in <i>B. pertussis</i> pathogenesis and its contribution to bacterial survival within immune cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104721
url http://sedici.unlp.edu.ar/handle/10915/104721
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/93561
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203204
info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0203204
info:eu-repo/semantics/altIdentifier/hdl/11336/93561
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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