Development of an immunochemical method to detect <i>Lactobacillus kefir</i>

Autores
Garrote, Graciela Liliana; Serradell, María de los Ángeles; Abraham, Analía Graciela; Añón, María Cristina; Fossati, Carlos Alberto; De Antoni, Graciela Liliana
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Kefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid way to quantify Lactobacillus kefir in a complex environment. Rabbit antisera against isolated S-layer proteins from L. kefir and L. parakefir were obtained. Both antisera recognized the 21 strains of L. kefir and the three strains of L. parakefir analysed by dot-blot. By indirect ELISA, specific antiserum against L. kefir S-layer showed reactivity against pure cultures of both L. kefir and L. parakefir, but did not react with L. brevis cells. Competitive ELISA allowed quantification of L. kefir at concentrations ranging from 5 × 10 5 to 108 bacteria ml-1, but neither L. parakefir nor L. brevis pure cultures produced inhibition. Through this approach it was possible to detect 108 L. kefir ml-1 in samples of milk fermented for 48 h at 20°C with different kefir grains. Competitive ELISA developed in our laboratory is a suitable method to detect and quantify L. kefir in milk and in consequence it could be applied to analyse the relationship between the composition of microflora and probiotic and technological characteristics of the products.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Facultad de Ciencias Exactas
Materia
Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83403
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83403
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Development of an immunochemical method to detect <i>Lactobacillus kefir</i>Garrote, Graciela LilianaSerradell, María de los ÁngelesAbraham, Analía GracielaAñón, María CristinaFossati, Carlos AlbertoDe Antoni, Graciela LilianaQuímicaELISAImmunodetectionKefir milkLactobacilliKefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid way to quantify Lactobacillus kefir in a complex environment. Rabbit antisera against isolated S-layer proteins from L. kefir and L. parakefir were obtained. Both antisera recognized the 21 strains of L. kefir and the three strains of L. parakefir analysed by dot-blot. By indirect ELISA, specific antiserum against L. kefir S-layer showed reactivity against pure cultures of both L. kefir and L. parakefir, but did not react with L. brevis cells. Competitive ELISA allowed quantification of L. kefir at concentrations ranging from 5 × 10 5 to 108 bacteria ml-1, but neither L. parakefir nor L. brevis pure cultures produced inhibition. Through this approach it was possible to detect 108 L. kefir ml-1 in samples of milk fermented for 48 h at 20°C with different kefir grains. Competitive ELISA developed in our laboratory is a suitable method to detect and quantify L. kefir in milk and in consequence it could be applied to analyse the relationship between the composition of microflora and probiotic and technological characteristics of the products.Centro de Investigación y Desarrollo en Criotecnología de AlimentosFacultad de Ciencias Exactas2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf221-233http://sedici.unlp.edu.ar/handle/10915/83403enginfo:eu-repo/semantics/altIdentifier/issn/0954-0105info:eu-repo/semantics/altIdentifier/doi/10.1080/09540100500244146info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:46Zoai:sedici.unlp.edu.ar:10915/83403Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:46.905SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
spellingShingle Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
Garrote, Graciela Liliana
Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
title_short Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_full Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_fullStr Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_full_unstemmed Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_sort Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
dc.creator.none.fl_str_mv Garrote, Graciela Liliana
Serradell, María de los Ángeles
Abraham, Analía Graciela
Añón, María Cristina
Fossati, Carlos Alberto
De Antoni, Graciela Liliana
author Garrote, Graciela Liliana
author_facet Garrote, Graciela Liliana
Serradell, María de los Ángeles
Abraham, Analía Graciela
Añón, María Cristina
Fossati, Carlos Alberto
De Antoni, Graciela Liliana
author_role author
author2 Serradell, María de los Ángeles
Abraham, Analía Graciela
Añón, María Cristina
Fossati, Carlos Alberto
De Antoni, Graciela Liliana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
topic Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
dc.description.none.fl_txt_mv Kefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid way to quantify Lactobacillus kefir in a complex environment. Rabbit antisera against isolated S-layer proteins from L. kefir and L. parakefir were obtained. Both antisera recognized the 21 strains of L. kefir and the three strains of L. parakefir analysed by dot-blot. By indirect ELISA, specific antiserum against L. kefir S-layer showed reactivity against pure cultures of both L. kefir and L. parakefir, but did not react with L. brevis cells. Competitive ELISA allowed quantification of L. kefir at concentrations ranging from 5 × 10 5 to 108 bacteria ml-1, but neither L. parakefir nor L. brevis pure cultures produced inhibition. Through this approach it was possible to detect 108 L. kefir ml-1 in samples of milk fermented for 48 h at 20°C with different kefir grains. Competitive ELISA developed in our laboratory is a suitable method to detect and quantify L. kefir in milk and in consequence it could be applied to analyse the relationship between the composition of microflora and probiotic and technological characteristics of the products.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Facultad de Ciencias Exactas
description Kefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid way to quantify Lactobacillus kefir in a complex environment. Rabbit antisera against isolated S-layer proteins from L. kefir and L. parakefir were obtained. Both antisera recognized the 21 strains of L. kefir and the three strains of L. parakefir analysed by dot-blot. By indirect ELISA, specific antiserum against L. kefir S-layer showed reactivity against pure cultures of both L. kefir and L. parakefir, but did not react with L. brevis cells. Competitive ELISA allowed quantification of L. kefir at concentrations ranging from 5 × 10 5 to 108 bacteria ml-1, but neither L. parakefir nor L. brevis pure cultures produced inhibition. Through this approach it was possible to detect 108 L. kefir ml-1 in samples of milk fermented for 48 h at 20°C with different kefir grains. Competitive ELISA developed in our laboratory is a suitable method to detect and quantify L. kefir in milk and in consequence it could be applied to analyse the relationship between the composition of microflora and probiotic and technological characteristics of the products.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83403
url http://sedici.unlp.edu.ar/handle/10915/83403
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0954-0105
info:eu-repo/semantics/altIdentifier/doi/10.1080/09540100500244146
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
221-233
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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