Characterization of the Na+/HCO3¯ cotransport in human neutrophils

Autores
Giambelluca, Miriam Soledad; Ciancio, María Carolina; Orlowski, Alejandro; Gende, Oscar Alfredo; Pouliot, Marc; Aiello, Ernesto Alejandro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pHi) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study. Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na+/H+ exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na+/HCO3¯ cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RT-PCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that neutrophils have an NBC that regulates basal pHi and is modulated by chemotactic agents.
Facultad de Ciencias Médicas
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85267
Acceder
id SEDICI_488f26e83ce292039cdcee0442bc99cb
oai_identifier_str oai:sedici.unlp.edu.ar:10915/85267
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Characterization of the Na+/HCO3¯ cotransport in human neutrophilsGiambelluca, Miriam SoledadCiancio, María CarolinaOrlowski, AlejandroGende, Oscar AlfredoPouliot, MarcAiello, Ernesto AlejandroCiencias MédicasBlood cellsIntracellular pHIon transportBackground: Bicarbonate transport has crucial roles in regulating intracellular pH (pH<SUB>i</SUB>) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study. Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na<SUP>+</SUP>/HCO<SUB>3</SUB>¯ cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RT-PCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that neutrophils have an NBC that regulates basal pH<SUB>i</SUB> and is modulated by chemotactic agents.Facultad de Ciencias MédicasCentro de Investigaciones Cardiovasculares2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf982-990http://sedici.unlp.edu.ar/handle/10915/85267enginfo:eu-repo/semantics/altIdentifier/issn/1015-8987info:eu-repo/semantics/altIdentifier/doi/10.1159/000358669info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T16:57:12Zoai:sedici.unlp.edu.ar:10915/85267Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 16:57:13.041SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title Characterization of the Na+/HCO3¯ cotransport in human neutrophils
spellingShingle Characterization of the Na+/HCO3¯ cotransport in human neutrophils
Giambelluca, Miriam Soledad
Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
title_short Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_full Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_fullStr Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_full_unstemmed Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_sort Characterization of the Na+/HCO3¯ cotransport in human neutrophils
dc.creator.none.fl_str_mv Giambelluca, Miriam Soledad
Ciancio, María Carolina
Orlowski, Alejandro
Gende, Oscar Alfredo
Pouliot, Marc
Aiello, Ernesto Alejandro
author Giambelluca, Miriam Soledad
author_facet Giambelluca, Miriam Soledad
Ciancio, María Carolina
Orlowski, Alejandro
Gende, Oscar Alfredo
Pouliot, Marc
Aiello, Ernesto Alejandro
author_role author
author2 Ciancio, María Carolina
Orlowski, Alejandro
Gende, Oscar Alfredo
Pouliot, Marc
Aiello, Ernesto Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
topic Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
dc.description.none.fl_txt_mv Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pH<SUB>i</SUB>) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study. Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na<SUP>+</SUP>/HCO<SUB>3</SUB>¯ cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RT-PCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that neutrophils have an NBC that regulates basal pH<SUB>i</SUB> and is modulated by chemotactic agents.
Facultad de Ciencias Médicas
Centro de Investigaciones Cardiovasculares
description Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pH<SUB>i</SUB>) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study. Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na<SUP>+</SUP>/HCO<SUB>3</SUB>¯ cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RT-PCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that neutrophils have an NBC that regulates basal pH<SUB>i</SUB> and is modulated by chemotactic agents.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85267
url http://sedici.unlp.edu.ar/handle/10915/85267
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1015-8987
info:eu-repo/semantics/altIdentifier/doi/10.1159/000358669
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
982-990
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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