Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils

Autores
Giambelluca, Miriam Soledad; Gende, Oscar Alfredo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils.
Fil: Giambelluca, Miriam Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Gende, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Materia
Chloride-Bicarbonate Exchanger
Neutrophils
Intracelular Ph
Chloride
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60871
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/60871
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophilsGiambelluca, Miriam SoledadGende, Oscar AlfredoChloride-Bicarbonate ExchangerNeutrophilsIntracelular PhChloridehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils.Fil: Giambelluca, Miriam Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaFil: Gende, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; ArgentinaAcademic Press Inc Elsevier Science2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60871Giambelluca, Miriam Soledad; Gende, Oscar Alfredo; Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 409; 3; 2011; 567-5710006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11008060info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2011.05.046info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:02Zoai:ri.conicet.gov.ar:11336/60871instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:02.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
title Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
spellingShingle Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
Giambelluca, Miriam Soledad
Chloride-Bicarbonate Exchanger
Neutrophils
Intracelular Ph
Chloride
title_short Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
title_full Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
title_fullStr Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
title_full_unstemmed Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
title_sort Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils
dc.creator.none.fl_str_mv Giambelluca, Miriam Soledad
Gende, Oscar Alfredo
author Giambelluca, Miriam Soledad
author_facet Giambelluca, Miriam Soledad
Gende, Oscar Alfredo
author_role author
author2 Gende, Oscar Alfredo
author2_role author
dc.subject.none.fl_str_mv Chloride-Bicarbonate Exchanger
Neutrophils
Intracelular Ph
Chloride
topic Chloride-Bicarbonate Exchanger
Neutrophils
Intracelular Ph
Chloride
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils.
Fil: Giambelluca, Miriam Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
Fil: Gende, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
description It is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60871
Giambelluca, Miriam Soledad; Gende, Oscar Alfredo; Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 409; 3; 2011; 567-571
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60871
identifier_str_mv Giambelluca, Miriam Soledad; Gende, Oscar Alfredo; Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 409; 3; 2011; 567-571
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11008060
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2011.05.046
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268705184022528
score 13.13397

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