Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats

Autores
Lehmann, Marianne; Zappa Villar, María Florencia; Garcia, Mariana; Mazzolini, Guillermo; Canatelli Mallat, Martina; Morel, Gustavo Ramón; Reggiani, Paula Cecilia; Goya, Rodolfo Gustavo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is a growing interest in the potential of adult stem cells for implementing regenerative medicine in the brain. We assessed the effect of intracerebroventricular (icv) administration of human umbilical cord perivascular cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using intact senile counterparts as controls. Approximately one third of the animals were injected in the lateral ventricles with a suspension containing 4.8 X 105 HUCPVC in 8 μl per side. The other third received 4.8 X 105 transgenic HUCPVC overexpressing Insulin-like growth factor-1 (IGF-1) and the last third of the rats received no treatment. Spatial memory performance was evaluated using a modified version of the Barnes maze test. In order to evaluate learning ability as well as spatial memory retention, we assessed the time spent (permanence) by animals in goal sector 1 (GS1) and 3 (GS3) when the escape box was removed. Fluorescence microscopy revealed the prescence of Dil-labeled HUCPVC in coronal sections of treated brains. The HUCPVC were located in close contact with the ependymal cells with only a few labeled cells migrating into the brain parenchyma. After treatment with naive or IGF-1 transgenic HUCPVC, permanence in GS1 and GS3 increased significantly whereas there were no changes in the intact animals. We conclude that HUCPVC injected icv are effective to improve some components of spatial memory in senile rats. The ready accessibility of HUCPVC constitutes a significant incentive to continue the exploration of their therapeutic potential on neurodegenerative diseases.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/142072
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/142072
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Umbilical Cord Cell Therapy Improves Spatial Memory in Aging RatsLehmann, MarianneZappa Villar, María FlorenciaGarcia, MarianaMazzolini, GuillermoCanatelli Mallat, MartinaMorel, Gustavo RamónReggiani, Paula CeciliaGoya, Rodolfo GustavoMedicinaBiologíaBrain agingSpatial memoryHippocampusUmbilical cordStem cellsThere is a growing interest in the potential of adult stem cells for implementing regenerative medicine in the brain. We assessed the effect of intracerebroventricular (icv) administration of human umbilical cord perivascular cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using intact senile counterparts as controls. Approximately one third of the animals were injected in the lateral ventricles with a suspension containing 4.8 X 105 HUCPVC in 8 μl per side. The other third received 4.8 X 105 transgenic HUCPVC overexpressing Insulin-like growth factor-1 (IGF-1) and the last third of the rats received no treatment. Spatial memory performance was evaluated using a modified version of the Barnes maze test. In order to evaluate learning ability as well as spatial memory retention, we assessed the time spent (permanence) by animals in goal sector 1 (GS1) and 3 (GS3) when the escape box was removed. Fluorescence microscopy revealed the prescence of Dil-labeled HUCPVC in coronal sections of treated brains. The HUCPVC were located in close contact with the ependymal cells with only a few labeled cells migrating into the brain parenchyma. After treatment with naive or IGF-1 transgenic HUCPVC, permanence in GS1 and GS3 increased significantly whereas there were no changes in the intact animals. We conclude that HUCPVC injected icv are effective to improve some components of spatial memory in senile rats. The ready accessibility of HUCPVC constitutes a significant incentive to continue the exploration of their therapeutic potential on neurodegenerative diseases.Instituto de Investigaciones Bioquímicas de La Plata2019-05-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf612-617http://sedici.unlp.edu.ar/handle/10915/142072enginfo:eu-repo/semantics/altIdentifier/issn/2629-3277info:eu-repo/semantics/altIdentifier/issn/1550-8943info:eu-repo/semantics/altIdentifier/issn/1558-6804info:eu-repo/semantics/altIdentifier/doi/10.1007/s12015-019-09895-2info:eu-repo/semantics/altIdentifier/pmid/31119513info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:18Zoai:sedici.unlp.edu.ar:10915/142072Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:18.424SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
spellingShingle Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
Lehmann, Marianne
Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
title_short Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_full Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_fullStr Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_full_unstemmed Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_sort Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
dc.creator.none.fl_str_mv Lehmann, Marianne
Zappa Villar, María Florencia
Garcia, Mariana
Mazzolini, Guillermo
Canatelli Mallat, Martina
Morel, Gustavo Ramón
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author Lehmann, Marianne
author_facet Lehmann, Marianne
Zappa Villar, María Florencia
Garcia, Mariana
Mazzolini, Guillermo
Canatelli Mallat, Martina
Morel, Gustavo Ramón
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author_role author
author2 Zappa Villar, María Florencia
Garcia, Mariana
Mazzolini, Guillermo
Canatelli Mallat, Martina
Morel, Gustavo Ramón
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
topic Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
dc.description.none.fl_txt_mv There is a growing interest in the potential of adult stem cells for implementing regenerative medicine in the brain. We assessed the effect of intracerebroventricular (icv) administration of human umbilical cord perivascular cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using intact senile counterparts as controls. Approximately one third of the animals were injected in the lateral ventricles with a suspension containing 4.8 X 105 HUCPVC in 8 μl per side. The other third received 4.8 X 105 transgenic HUCPVC overexpressing Insulin-like growth factor-1 (IGF-1) and the last third of the rats received no treatment. Spatial memory performance was evaluated using a modified version of the Barnes maze test. In order to evaluate learning ability as well as spatial memory retention, we assessed the time spent (permanence) by animals in goal sector 1 (GS1) and 3 (GS3) when the escape box was removed. Fluorescence microscopy revealed the prescence of Dil-labeled HUCPVC in coronal sections of treated brains. The HUCPVC were located in close contact with the ependymal cells with only a few labeled cells migrating into the brain parenchyma. After treatment with naive or IGF-1 transgenic HUCPVC, permanence in GS1 and GS3 increased significantly whereas there were no changes in the intact animals. We conclude that HUCPVC injected icv are effective to improve some components of spatial memory in senile rats. The ready accessibility of HUCPVC constitutes a significant incentive to continue the exploration of their therapeutic potential on neurodegenerative diseases.
Instituto de Investigaciones Bioquímicas de La Plata
description There is a growing interest in the potential of adult stem cells for implementing regenerative medicine in the brain. We assessed the effect of intracerebroventricular (icv) administration of human umbilical cord perivascular cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using intact senile counterparts as controls. Approximately one third of the animals were injected in the lateral ventricles with a suspension containing 4.8 X 105 HUCPVC in 8 μl per side. The other third received 4.8 X 105 transgenic HUCPVC overexpressing Insulin-like growth factor-1 (IGF-1) and the last third of the rats received no treatment. Spatial memory performance was evaluated using a modified version of the Barnes maze test. In order to evaluate learning ability as well as spatial memory retention, we assessed the time spent (permanence) by animals in goal sector 1 (GS1) and 3 (GS3) when the escape box was removed. Fluorescence microscopy revealed the prescence of Dil-labeled HUCPVC in coronal sections of treated brains. The HUCPVC were located in close contact with the ependymal cells with only a few labeled cells migrating into the brain parenchyma. After treatment with naive or IGF-1 transgenic HUCPVC, permanence in GS1 and GS3 increased significantly whereas there were no changes in the intact animals. We conclude that HUCPVC injected icv are effective to improve some components of spatial memory in senile rats. The ready accessibility of HUCPVC constitutes a significant incentive to continue the exploration of their therapeutic potential on neurodegenerative diseases.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/142072
url http://sedici.unlp.edu.ar/handle/10915/142072
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2629-3277
info:eu-repo/semantics/altIdentifier/issn/1550-8943
info:eu-repo/semantics/altIdentifier/issn/1558-6804
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12015-019-09895-2
info:eu-repo/semantics/altIdentifier/pmid/31119513
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
612-617
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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