Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells

Autores
Iribarne, Ailen; Palma, Maria Belen; Andrini, Laura Beatríz; Riccillo, Fernando Luis; Rodriguez Juiz, Delfina; Casella, Martín Franco; Garay, Felipe; Spoto Zabala, Julieta; Mazza, Leandro; Muro, Adriana; Buero, Guillermo; Miriuka, Santiago Gabriel; Carosella, Edgardo Delfino; García, Marcela Nilda
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton’s jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.
Fil: Iribarne, Ailen. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Fil: Palma, Maria Belen. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Andrini, Laura Beatríz. Fundacion P/la Lucha C/enferm.neurologicas Infancia. Instituto de Neurociencias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Neurociencias.; Argentina
Fil: Riccillo, Fernando Luis. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Fil: Rodriguez Juiz, Delfina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Fil: Casella, Martín Franco. División Remonta Haras La Teruca; Argentina
Fil: Garay, Felipe. División Remonta Haras la Teruca; Argentina
Fil: Spoto Zabala, Julieta. División Remonta Haras la Teruca; Argentina
Fil: Mazza, Leandro. Dirección Veterinaria, Policía de la Provincia de Bs As; Argentina
Fil: Muro, Adriana. División Remonta Haras la Teruca; Argentina
Fil: Buero, Guillermo. Policía de la Provincia de Bs As; Argentina
Fil: Miriuka, Santiago Gabriel. Cátedra de Anatomía B; Argentina
Fil: Carosella, Edgardo Delfino. Laboratorio de Análisis Clínicos Veterinarios, la Plata; Argentina
Fil: García, Marcela Nilda. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Materia
HORSES
WOUND HEALING
MESENCHYMAL STEM CELLS
CELLULAR THERAPY
EQUINE UMBILICAL CORD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269304
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem CellsIribarne, AilenPalma, Maria BelenAndrini, Laura BeatrízRiccillo, Fernando LuisRodriguez Juiz, DelfinaCasella, Martín FrancoGaray, FelipeSpoto Zabala, JulietaMazza, LeandroMuro, AdrianaBuero, GuillermoMiriuka, Santiago GabrielCarosella, Edgardo DelfinoGarcía, Marcela NildaHORSESWOUND HEALINGMESENCHYMAL STEM CELLSCELLULAR THERAPYEQUINE UMBILICAL CORDhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton’s jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.Fil: Iribarne, Ailen. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; ArgentinaFil: Palma, Maria Belen. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Andrini, Laura Beatríz. Fundacion P/la Lucha C/enferm.neurologicas Infancia. Instituto de Neurociencias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Neurociencias.; ArgentinaFil: Riccillo, Fernando Luis. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; ArgentinaFil: Rodriguez Juiz, Delfina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; ArgentinaFil: Casella, Martín Franco. División Remonta Haras La Teruca; ArgentinaFil: Garay, Felipe. División Remonta Haras la Teruca; ArgentinaFil: Spoto Zabala, Julieta. División Remonta Haras la Teruca; ArgentinaFil: Mazza, Leandro. Dirección Veterinaria, Policía de la Provincia de Bs As; ArgentinaFil: Muro, Adriana. División Remonta Haras la Teruca; ArgentinaFil: Buero, Guillermo. Policía de la Provincia de Bs As; ArgentinaFil: Miriuka, Santiago Gabriel. Cátedra de Anatomía B; ArgentinaFil: Carosella, Edgardo Delfino. Laboratorio de Análisis Clínicos Veterinarios, la Plata; ArgentinaFil: García, Marcela Nilda. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; ArgentinaMolecular Diversity Preservation International2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269304Iribarne, Ailen; Palma, Maria Belen; Andrini, Laura Beatríz; Riccillo, Fernando Luis; Rodriguez Juiz, Delfina; et al.; Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 4; 2-20241422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/4/2350info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25042350info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:00:31Zoai:ri.conicet.gov.ar:11336/269304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:00:32.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
title Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
spellingShingle Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
Iribarne, Ailen
HORSES
WOUND HEALING
MESENCHYMAL STEM CELLS
CELLULAR THERAPY
EQUINE UMBILICAL CORD
title_short Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
title_full Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
title_fullStr Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
title_full_unstemmed Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
title_sort Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
dc.creator.none.fl_str_mv Iribarne, Ailen
Palma, Maria Belen
Andrini, Laura Beatríz
Riccillo, Fernando Luis
Rodriguez Juiz, Delfina
Casella, Martín Franco
Garay, Felipe
Spoto Zabala, Julieta
Mazza, Leandro
Muro, Adriana
Buero, Guillermo
Miriuka, Santiago Gabriel
Carosella, Edgardo Delfino
García, Marcela Nilda
author Iribarne, Ailen
author_facet Iribarne, Ailen
Palma, Maria Belen
Andrini, Laura Beatríz
Riccillo, Fernando Luis
Rodriguez Juiz, Delfina
Casella, Martín Franco
Garay, Felipe
Spoto Zabala, Julieta
Mazza, Leandro
Muro, Adriana
Buero, Guillermo
Miriuka, Santiago Gabriel
Carosella, Edgardo Delfino
García, Marcela Nilda
author_role author
author2 Palma, Maria Belen
Andrini, Laura Beatríz
Riccillo, Fernando Luis
Rodriguez Juiz, Delfina
Casella, Martín Franco
Garay, Felipe
Spoto Zabala, Julieta
Mazza, Leandro
Muro, Adriana
Buero, Guillermo
Miriuka, Santiago Gabriel
Carosella, Edgardo Delfino
García, Marcela Nilda
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HORSES
WOUND HEALING
MESENCHYMAL STEM CELLS
CELLULAR THERAPY
EQUINE UMBILICAL CORD
topic HORSES
WOUND HEALING
MESENCHYMAL STEM CELLS
CELLULAR THERAPY
EQUINE UMBILICAL CORD
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton’s jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.
Fil: Iribarne, Ailen. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Fil: Palma, Maria Belen. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Andrini, Laura Beatríz. Fundacion P/la Lucha C/enferm.neurologicas Infancia. Instituto de Neurociencias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Neurociencias.; Argentina
Fil: Riccillo, Fernando Luis. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Fil: Rodriguez Juiz, Delfina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
Fil: Casella, Martín Franco. División Remonta Haras La Teruca; Argentina
Fil: Garay, Felipe. División Remonta Haras la Teruca; Argentina
Fil: Spoto Zabala, Julieta. División Remonta Haras la Teruca; Argentina
Fil: Mazza, Leandro. Dirección Veterinaria, Policía de la Provincia de Bs As; Argentina
Fil: Muro, Adriana. División Remonta Haras la Teruca; Argentina
Fil: Buero, Guillermo. Policía de la Provincia de Bs As; Argentina
Fil: Miriuka, Santiago Gabriel. Cátedra de Anatomía B; Argentina
Fil: Carosella, Edgardo Delfino. Laboratorio de Análisis Clínicos Veterinarios, la Plata; Argentina
Fil: García, Marcela Nilda. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Ciencias Morfológicas. Cátedra de Citología y Embriología A; Argentina
description Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton’s jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269304
Iribarne, Ailen; Palma, Maria Belen; Andrini, Laura Beatríz; Riccillo, Fernando Luis; Rodriguez Juiz, Delfina; et al.; Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 4; 2-2024
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269304
identifier_str_mv Iribarne, Ailen; Palma, Maria Belen; Andrini, Laura Beatríz; Riccillo, Fernando Luis; Rodriguez Juiz, Delfina; et al.; Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 4; 2-2024
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/4/2350
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25042350
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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