Intra-colonial viral infections in western honey bees (Apis Mellifera)

Autores
Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; Invernizzi, Ciro; Reynaldi, Francisco José; Le Conte, Yves; Beaurepaire, Alexis
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
Instituto Multidisciplinario de Biología Celular
Laboratorio de Virología
Materia
Biología
Evolutionary biology
Host–pathogen interactions
Population genetics
Viruses
Pollinators
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/125408
Acceder
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network_name_str SEDICI (UNLP)
spelling Intra-colonial viral infections in western honey bees (Apis Mellifera)Castelli, LoreleyGenchi García, María LauraDalmon, AnneArredondo, DanielaAntúnez, KarinaInvernizzi, CiroReynaldi, Francisco JoséLe Conte, YvesBeaurepaire, AlexisBiologíaEvolutionary biologyHost–pathogen interactionsPopulation geneticsVirusesPollinatorsRNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.Instituto Multidisciplinario de Biología CelularLaboratorio de Virología2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/125408enginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/5/1087info:eu-repo/semantics/altIdentifier/issn/2076-2607info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9051087info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:03Zoai:sedici.unlp.edu.ar:10915/125408Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:03.588SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Intra-colonial viral infections in western honey bees (Apis Mellifera)
title Intra-colonial viral infections in western honey bees (Apis Mellifera)
spellingShingle Intra-colonial viral infections in western honey bees (Apis Mellifera)
Castelli, Loreley
Biología
Evolutionary biology
Host–pathogen interactions
Population genetics
Viruses
Pollinators
title_short Intra-colonial viral infections in western honey bees (Apis Mellifera)
title_full Intra-colonial viral infections in western honey bees (Apis Mellifera)
title_fullStr Intra-colonial viral infections in western honey bees (Apis Mellifera)
title_full_unstemmed Intra-colonial viral infections in western honey bees (Apis Mellifera)
title_sort Intra-colonial viral infections in western honey bees (Apis Mellifera)
dc.creator.none.fl_str_mv Castelli, Loreley
Genchi García, María Laura
Dalmon, Anne
Arredondo, Daniela
Antúnez, Karina
Invernizzi, Ciro
Reynaldi, Francisco José
Le Conte, Yves
Beaurepaire, Alexis
author Castelli, Loreley
author_facet Castelli, Loreley
Genchi García, María Laura
Dalmon, Anne
Arredondo, Daniela
Antúnez, Karina
Invernizzi, Ciro
Reynaldi, Francisco José
Le Conte, Yves
Beaurepaire, Alexis
author_role author
author2 Genchi García, María Laura
Dalmon, Anne
Arredondo, Daniela
Antúnez, Karina
Invernizzi, Ciro
Reynaldi, Francisco José
Le Conte, Yves
Beaurepaire, Alexis
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Evolutionary biology
Host–pathogen interactions
Population genetics
Viruses
Pollinators
topic Biología
Evolutionary biology
Host–pathogen interactions
Population genetics
Viruses
Pollinators
dc.description.none.fl_txt_mv RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
Instituto Multidisciplinario de Biología Celular
Laboratorio de Virología
description RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/125408
url http://sedici.unlp.edu.ar/handle/10915/125408
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/5/1087
info:eu-repo/semantics/altIdentifier/issn/2076-2607
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9051087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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