Intra-colonial viral infections in western honey bees (Apis mellifera)

Autores
Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; Invernizzi, Ciro; Reynaldi, Francisco José; Le Conte, Yves; Beaurepaire, Alexis
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
Fil: Castelli, Loreley. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Genchi García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Dalmon, Anne. Institut National de la Recherche Agronomique; Francia
Fil: Arredondo, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Invernizzi, Ciro. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Le Conte, Yves. Institut National de la Recherche Agronomique; Francia
Fil: Beaurepaire, Alexis. University of Bern; Suiza. Institut National de la Recherche Agronomique; Francia
Materia
EVOLUTIONARY BIOLOGY
HOST–PATHOGEN INTERACTIONS
POLLINATORS
POPULATION GENETICS
VIRUSES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150265
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/150265
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intra-colonial viral infections in western honey bees (Apis mellifera)Castelli, LoreleyGenchi García, María LauraDalmon, AnneArredondo, DanielaAntúnez, KarinaInvernizzi, CiroReynaldi, Francisco JoséLe Conte, YvesBeaurepaire, AlexisEVOLUTIONARY BIOLOGYHOST–PATHOGEN INTERACTIONSPOLLINATORSPOPULATION GENETICSVIRUSEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.Fil: Castelli, Loreley. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Genchi García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Dalmon, Anne. Institut National de la Recherche Agronomique; FranciaFil: Arredondo, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Invernizzi, Ciro. Universidad de la República. Facultad de Ciencias; UruguayFil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Le Conte, Yves. Institut National de la Recherche Agronomique; FranciaFil: Beaurepaire, Alexis. University of Bern; Suiza. Institut National de la Recherche Agronomique; FranciaMDPI2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150265Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; et al.; Intra-colonial viral infections in western honey bees (Apis mellifera); MDPI; Microorganisms; 9; 5; 5-2021; 1-122076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/5/1087info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9051087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:59Zoai:ri.conicet.gov.ar:11336/150265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:59.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intra-colonial viral infections in western honey bees (Apis mellifera)
title Intra-colonial viral infections in western honey bees (Apis mellifera)
spellingShingle Intra-colonial viral infections in western honey bees (Apis mellifera)
Castelli, Loreley
EVOLUTIONARY BIOLOGY
HOST–PATHOGEN INTERACTIONS
POLLINATORS
POPULATION GENETICS
VIRUSES
title_short Intra-colonial viral infections in western honey bees (Apis mellifera)
title_full Intra-colonial viral infections in western honey bees (Apis mellifera)
title_fullStr Intra-colonial viral infections in western honey bees (Apis mellifera)
title_full_unstemmed Intra-colonial viral infections in western honey bees (Apis mellifera)
title_sort Intra-colonial viral infections in western honey bees (Apis mellifera)
dc.creator.none.fl_str_mv Castelli, Loreley
Genchi García, María Laura
Dalmon, Anne
Arredondo, Daniela
Antúnez, Karina
Invernizzi, Ciro
Reynaldi, Francisco José
Le Conte, Yves
Beaurepaire, Alexis
author Castelli, Loreley
author_facet Castelli, Loreley
Genchi García, María Laura
Dalmon, Anne
Arredondo, Daniela
Antúnez, Karina
Invernizzi, Ciro
Reynaldi, Francisco José
Le Conte, Yves
Beaurepaire, Alexis
author_role author
author2 Genchi García, María Laura
Dalmon, Anne
Arredondo, Daniela
Antúnez, Karina
Invernizzi, Ciro
Reynaldi, Francisco José
Le Conte, Yves
Beaurepaire, Alexis
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EVOLUTIONARY BIOLOGY
HOST–PATHOGEN INTERACTIONS
POLLINATORS
POPULATION GENETICS
VIRUSES
topic EVOLUTIONARY BIOLOGY
HOST–PATHOGEN INTERACTIONS
POLLINATORS
POPULATION GENETICS
VIRUSES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
Fil: Castelli, Loreley. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Genchi García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Dalmon, Anne. Institut National de la Recherche Agronomique; Francia
Fil: Arredondo, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Invernizzi, Ciro. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Le Conte, Yves. Institut National de la Recherche Agronomique; Francia
Fil: Beaurepaire, Alexis. University of Bern; Suiza. Institut National de la Recherche Agronomique; Francia
description RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150265
Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; et al.; Intra-colonial viral infections in western honey bees (Apis mellifera); MDPI; Microorganisms; 9; 5; 5-2021; 1-12
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150265
identifier_str_mv Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; et al.; Intra-colonial viral infections in western honey bees (Apis mellifera); MDPI; Microorganisms; 9; 5; 5-2021; 1-12
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/5/1087
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9051087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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