Intra-colonial viral infections in western honey bees (Apis mellifera)
- Autores
- Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; Invernizzi, Ciro; Reynaldi, Francisco José; Le Conte, Yves; Beaurepaire, Alexis
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
Fil: Castelli, Loreley. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Genchi García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Dalmon, Anne. Institut National de la Recherche Agronomique; Francia
Fil: Arredondo, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Invernizzi, Ciro. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Le Conte, Yves. Institut National de la Recherche Agronomique; Francia
Fil: Beaurepaire, Alexis. University of Bern; Suiza. Institut National de la Recherche Agronomique; Francia - Materia
-
EVOLUTIONARY BIOLOGY
HOST–PATHOGEN INTERACTIONS
POLLINATORS
POPULATION GENETICS
VIRUSES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/150265
Ver los metadatos del registro completo
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Intra-colonial viral infections in western honey bees (Apis mellifera)Castelli, LoreleyGenchi García, María LauraDalmon, AnneArredondo, DanielaAntúnez, KarinaInvernizzi, CiroReynaldi, Francisco JoséLe Conte, YvesBeaurepaire, AlexisEVOLUTIONARY BIOLOGYHOST–PATHOGEN INTERACTIONSPOLLINATORSPOPULATION GENETICSVIRUSEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.Fil: Castelli, Loreley. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Genchi García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Dalmon, Anne. Institut National de la Recherche Agronomique; FranciaFil: Arredondo, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Invernizzi, Ciro. Universidad de la República. Facultad de Ciencias; UruguayFil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Le Conte, Yves. Institut National de la Recherche Agronomique; FranciaFil: Beaurepaire, Alexis. University of Bern; Suiza. Institut National de la Recherche Agronomique; FranciaMDPI2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150265Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; et al.; Intra-colonial viral infections in western honey bees (Apis mellifera); MDPI; Microorganisms; 9; 5; 5-2021; 1-122076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/5/1087info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9051087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:37Zoai:ri.conicet.gov.ar:11336/150265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:38.119CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
title |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
spellingShingle |
Intra-colonial viral infections in western honey bees (Apis mellifera) Castelli, Loreley EVOLUTIONARY BIOLOGY HOST–PATHOGEN INTERACTIONS POLLINATORS POPULATION GENETICS VIRUSES |
title_short |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
title_full |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
title_fullStr |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
title_full_unstemmed |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
title_sort |
Intra-colonial viral infections in western honey bees (Apis mellifera) |
dc.creator.none.fl_str_mv |
Castelli, Loreley Genchi García, María Laura Dalmon, Anne Arredondo, Daniela Antúnez, Karina Invernizzi, Ciro Reynaldi, Francisco José Le Conte, Yves Beaurepaire, Alexis |
author |
Castelli, Loreley |
author_facet |
Castelli, Loreley Genchi García, María Laura Dalmon, Anne Arredondo, Daniela Antúnez, Karina Invernizzi, Ciro Reynaldi, Francisco José Le Conte, Yves Beaurepaire, Alexis |
author_role |
author |
author2 |
Genchi García, María Laura Dalmon, Anne Arredondo, Daniela Antúnez, Karina Invernizzi, Ciro Reynaldi, Francisco José Le Conte, Yves Beaurepaire, Alexis |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
EVOLUTIONARY BIOLOGY HOST–PATHOGEN INTERACTIONS POLLINATORS POPULATION GENETICS VIRUSES |
topic |
EVOLUTIONARY BIOLOGY HOST–PATHOGEN INTERACTIONS POLLINATORS POPULATION GENETICS VIRUSES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects. Fil: Castelli, Loreley. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Genchi García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina Fil: Dalmon, Anne. Institut National de la Recherche Agronomique; Francia Fil: Arredondo, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Invernizzi, Ciro. Universidad de la República. Facultad de Ciencias; Uruguay Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Le Conte, Yves. Institut National de la Recherche Agronomique; Francia Fil: Beaurepaire, Alexis. University of Bern; Suiza. Institut National de la Recherche Agronomique; Francia |
description |
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor-parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/150265 Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; et al.; Intra-colonial viral infections in western honey bees (Apis mellifera); MDPI; Microorganisms; 9; 5; 5-2021; 1-12 2076-2607 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/150265 |
identifier_str_mv |
Castelli, Loreley; Genchi García, María Laura; Dalmon, Anne; Arredondo, Daniela; Antúnez, Karina; et al.; Intra-colonial viral infections in western honey bees (Apis mellifera); MDPI; Microorganisms; 9; 5; 5-2021; 1-12 2076-2607 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/5/1087 info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9051087 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
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application/pdf application/pdf |
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MDPI |
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MDPI |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |