Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina

Autores
Toledo, Andrea Vanesa; Simurro, María Eugenia; Balatti, Pedro Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Cixiidae, Delphacodes kuscheli, Ectopsocidae, entomopathogenic fungi, Hirsutella citriformis, internal transcribed spacer
Hongos
Insectos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/66682
Acceder
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network_name_str SEDICI (UNLP)
spelling Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in ArgentinaToledo, Andrea VanesaSimurro, María EugeniaBalatti, Pedro AlbertoCiencias AgrariasCixiidae, Delphacodes kuscheli, Ectopsocidae, entomopathogenic fungi, Hirsutella citriformis, internal transcribed spacerHongosInsectosA mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.Facultad de Ciencias Agrarias y Forestales2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/66682enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jinsectscience/article/13/1/18/1067220info:eu-repo/semantics/altIdentifier/issn/1536-2442info:eu-repo/semantics/altIdentifier/doi/10.1673/031.013.1801info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:10:01Zoai:sedici.unlp.edu.ar:10915/66682Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:10:01.315SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
title Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
spellingShingle Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
Toledo, Andrea Vanesa
Ciencias Agrarias
Cixiidae, Delphacodes kuscheli, Ectopsocidae, entomopathogenic fungi, Hirsutella citriformis, internal transcribed spacer
Hongos
Insectos
title_short Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
title_full Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
title_fullStr Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
title_full_unstemmed Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
title_sort Morphological and molecular characterization of a fungus, <i>Hirsutella</i> sp., isolated from planthoppers and psocids in Argentina
dc.creator.none.fl_str_mv Toledo, Andrea Vanesa
Simurro, María Eugenia
Balatti, Pedro Alberto
author Toledo, Andrea Vanesa
author_facet Toledo, Andrea Vanesa
Simurro, María Eugenia
Balatti, Pedro Alberto
author_role author
author2 Simurro, María Eugenia
Balatti, Pedro Alberto
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Cixiidae, Delphacodes kuscheli, Ectopsocidae, entomopathogenic fungi, Hirsutella citriformis, internal transcribed spacer
Hongos
Insectos
topic Ciencias Agrarias
Cixiidae, Delphacodes kuscheli, Ectopsocidae, entomopathogenic fungi, Hirsutella citriformis, internal transcribed spacer
Hongos
Insectos
dc.description.none.fl_txt_mv A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.
Facultad de Ciencias Agrarias y Forestales
description A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/66682
url http://sedici.unlp.edu.ar/handle/10915/66682
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jinsectscience/article/13/1/18/1067220
info:eu-repo/semantics/altIdentifier/issn/1536-2442
info:eu-repo/semantics/altIdentifier/doi/10.1673/031.013.1801
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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