Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina

Autores
Toledo, Andrea Vanesa; Simurro, María Eugenia; Balatti, Pedro Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.
Fil: Toledo, Andrea Vanesa. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Simurro, María Eugenia. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Balatti, Pedro Alberto. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CIXIIDAE
ECTOPSOCIDAE
ENTOMOPATHOGENIC FUNGI
HIRSUTELLA CITRIFORMIS
INTERNAL TRANSCRIBED SPACER
DELPHACODES KUSCHELI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21921
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21921
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in ArgentinaToledo, Andrea VanesaSimurro, María EugeniaBalatti, Pedro AlbertoCIXIIDAEECTOPSOCIDAEENTOMOPATHOGENIC FUNGIHIRSUTELLA CITRIFORMISINTERNAL TRANSCRIBED SPACERDELPHACODES KUSCHELIhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.Fil: Toledo, Andrea Vanesa. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Simurro, María Eugenia. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Balatti, Pedro Alberto. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaOxford University Press2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21921Toledo, Andrea Vanesa; Simurro, María Eugenia; Balatti, Pedro Alberto; Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina; Oxford University Press; Journal of Insect Science; 13; 1; 1-2013; 1-10; 181536-2442CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1673/031.013.1801info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jinsectscience/article-lookup/doi/10.1673/031.013.1801info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:56Zoai:ri.conicet.gov.ar:11336/21921instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:57.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
title Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
spellingShingle Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
Toledo, Andrea Vanesa
CIXIIDAE
ECTOPSOCIDAE
ENTOMOPATHOGENIC FUNGI
HIRSUTELLA CITRIFORMIS
INTERNAL TRANSCRIBED SPACER
DELPHACODES KUSCHELI
title_short Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
title_full Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
title_fullStr Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
title_full_unstemmed Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
title_sort Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina
dc.creator.none.fl_str_mv Toledo, Andrea Vanesa
Simurro, María Eugenia
Balatti, Pedro Alberto
author Toledo, Andrea Vanesa
author_facet Toledo, Andrea Vanesa
Simurro, María Eugenia
Balatti, Pedro Alberto
author_role author
author2 Simurro, María Eugenia
Balatti, Pedro Alberto
author2_role author
author
dc.subject.none.fl_str_mv CIXIIDAE
ECTOPSOCIDAE
ENTOMOPATHOGENIC FUNGI
HIRSUTELLA CITRIFORMIS
INTERNAL TRANSCRIBED SPACER
DELPHACODES KUSCHELI
topic CIXIIDAE
ECTOPSOCIDAE
ENTOMOPATHOGENIC FUNGI
HIRSUTELLA CITRIFORMIS
INTERNAL TRANSCRIBED SPACER
DELPHACODES KUSCHELI
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.
Fil: Toledo, Andrea Vanesa. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Simurro, María Eugenia. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Balatti, Pedro Alberto. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Cs.biológicas. Centro de Invest.de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Invest.de Fitopatologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A mycosed planthopper, Oliarus dimidiatus Berg (Hemiptera: Cixiidae), and two psocids, Heterocaecilius sp. (Psocodea: Pseudocaeciliidae) and Ectopsocus sp. (Ectopsocidae), were collected from Los Hornos and La Plata, Buenos Aires, Argentina between February and September 2007. Observations of mycelia growing on the host revealed that the putative fungal parasite had synnemata supporting monophialidic conidiogenous cells. Likewise, in vitro fungal cultures presented characteristics typical of the fungus Hirsutella citriformis Speare (Ascomycota: Hypocreales: Clavicipitaceae). The identity of the isolated fungi characterized based on morphological aspects was complemented by means of the internal transcribed spacer sequences. The sequences of both isolates were highly homologous to those of Cordyceps sp. (Fries) Link and Ophiocordyceps sinensis (Berkely) G.H. Sung, J.M. Sung, Hywel-Jones, and Spatafora (Ophiocordycipitaceae). We additionally confirmed that both isolates had the ability to infect and kill adults of Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite insects, we conclude that the fungi isolated belong to the genus Hirsutella and might have biotechnological potential.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21921
Toledo, Andrea Vanesa; Simurro, María Eugenia; Balatti, Pedro Alberto; Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina; Oxford University Press; Journal of Insect Science; 13; 1; 1-2013; 1-10; 18
1536-2442
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21921
identifier_str_mv Toledo, Andrea Vanesa; Simurro, María Eugenia; Balatti, Pedro Alberto; Morphological and molecular characterization of a fungus, Hirsutella sp., isolated from planthoppers and psocids in Argentina; Oxford University Press; Journal of Insect Science; 13; 1; 1-2013; 1-10; 18
1536-2442
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1673/031.013.1801
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jinsectscience/article-lookup/doi/10.1673/031.013.1801
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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