First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
- Autores
- Serena, María Soledad; Metz, Germán Ernesto; Lozada, María Inés; Aspitia, Carolina Gabriela; Nicolino, Edgardo Héctor; Pidone, Claudio Luis; Fossaroli, Melisa Gisele; Balsalobre, Agustín; Quiroga, María Alejandra; Echeverría, María Gabriela
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
Facultad de Ciencias Veterinarias - Materia
-
Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/93228
Ver los metadatos del registro completo
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First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in ArgentinaSerena, María SoledadMetz, Germán ErnestoLozada, María InésAspitia, Carolina GabrielaNicolino, Edgardo HéctorPidone, Claudio LuisFossaroli, Melisa GiseleBalsalobre, AgustínQuiroga, María AlejandraEcheverría, María GabrielaCiencias VeterinariasArgentinaDomestic dogSuid herpesvirus 1Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.Facultad de Ciencias Veterinarias2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf131-139http://sedici.unlp.edu.ar/handle/10915/93228enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/handle/11336/94583info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/169608info:eu-repo/semantics/altIdentifier/issn/2226-4485info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i2.3info:eu-repo/semantics/altIdentifier/hdl/11336/94583info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:19:17Zoai:sedici.unlp.edu.ar:10915/93228Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:19:18.056SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
title |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
spellingShingle |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina Serena, María Soledad Ciencias Veterinarias Argentina Domestic dog Suid herpesvirus 1 |
title_short |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
title_full |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
title_fullStr |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
title_full_unstemmed |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
title_sort |
First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina |
dc.creator.none.fl_str_mv |
Serena, María Soledad Metz, Germán Ernesto Lozada, María Inés Aspitia, Carolina Gabriela Nicolino, Edgardo Héctor Pidone, Claudio Luis Fossaroli, Melisa Gisele Balsalobre, Agustín Quiroga, María Alejandra Echeverría, María Gabriela |
author |
Serena, María Soledad |
author_facet |
Serena, María Soledad Metz, Germán Ernesto Lozada, María Inés Aspitia, Carolina Gabriela Nicolino, Edgardo Héctor Pidone, Claudio Luis Fossaroli, Melisa Gisele Balsalobre, Agustín Quiroga, María Alejandra Echeverría, María Gabriela |
author_role |
author |
author2 |
Metz, Germán Ernesto Lozada, María Inés Aspitia, Carolina Gabriela Nicolino, Edgardo Héctor Pidone, Claudio Luis Fossaroli, Melisa Gisele Balsalobre, Agustín Quiroga, María Alejandra Echeverría, María Gabriela |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Argentina Domestic dog Suid herpesvirus 1 |
topic |
Ciencias Veterinarias Argentina Domestic dog Suid herpesvirus 1 |
dc.description.none.fl_txt_mv |
Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains. Facultad de Ciencias Veterinarias |
description |
Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/93228 |
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http://sedici.unlp.edu.ar/handle/10915/93228 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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