First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Autores
Serena, María Soledad; Metz, Germán Ernesto; Lozada, María Inés; Aspitia, Carolina Gabriela; Nicolino, Edgardo Héctor; Pidone, Claudio Luis; Fossaroli, Melisa Gisele; Balsalobre, Agustín; Quiroga, María Alejandra; Echeverría, María Gabriela
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/93228

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spelling First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in ArgentinaSerena, María SoledadMetz, Germán ErnestoLozada, María InésAspitia, Carolina GabrielaNicolino, Edgardo HéctorPidone, Claudio LuisFossaroli, Melisa GiseleBalsalobre, AgustínQuiroga, María AlejandraEcheverría, María GabrielaCiencias VeterinariasArgentinaDomestic dogSuid herpesvirus 1Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.Facultad de Ciencias Veterinarias2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf131-139http://sedici.unlp.edu.ar/handle/10915/93228enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/handle/11336/94583info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/169608info:eu-repo/semantics/altIdentifier/issn/2226-4485info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i2.3info:eu-repo/semantics/altIdentifier/hdl/11336/94583info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:19:17Zoai:sedici.unlp.edu.ar:10915/93228Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:19:18.056SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
spellingShingle First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
Serena, María Soledad
Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
title_short First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_full First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_fullStr First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_full_unstemmed First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_sort First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
dc.creator.none.fl_str_mv Serena, María Soledad
Metz, Germán Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Héctor
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustín
Quiroga, María Alejandra
Echeverría, María Gabriela
author Serena, María Soledad
author_facet Serena, María Soledad
Metz, Germán Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Héctor
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustín
Quiroga, María Alejandra
Echeverría, María Gabriela
author_role author
author2 Metz, Germán Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Héctor
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustín
Quiroga, María Alejandra
Echeverría, María Gabriela
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
topic Ciencias Veterinarias
Argentina
Domestic dog
Suid herpesvirus 1
dc.description.none.fl_txt_mv Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
Facultad de Ciencias Veterinarias
description Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.language.none.fl_str_mv eng
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info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/169608
info:eu-repo/semantics/altIdentifier/issn/2226-4485
info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i2.3
info:eu-repo/semantics/altIdentifier/hdl/11336/94583
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
131-139
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