First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Autores
Serena, Maria Soledad; Metz, German Ernesto; Lozada, María Inés; Aspitia, Carolina Gabriela; Nicolino, Edgardo Hector; Pidone, Claudio Luis; Fossaroli, Melisa Gisele; Balsalobre, Agustin; Quiroga, María Alejandra; Echeverria, Maria Gabriela
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Lozada, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina
Fil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina
Fil: Nicolino, Edgardo Hector. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; Argentina
Fil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; Argentina
Fil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; Argentina
Fil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Materia
ARGENTINA
DOMESTIC DOG
SUID HERPESVIRUS 1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94583
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in ArgentinaSerena, Maria SoledadMetz, German ErnestoLozada, María InésAspitia, Carolina GabrielaNicolino, Edgardo HectorPidone, Claudio LuisFossaroli, Melisa GiseleBalsalobre, AgustinQuiroga, María AlejandraEcheverria, Maria GabrielaARGENTINADOMESTIC DOGSUID HERPESVIRUS 1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Lozada, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; ArgentinaFil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; ArgentinaFil: Nicolino, Edgardo Hector. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; ArgentinaFil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; ArgentinaFil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; ArgentinaFil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quiroga, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaUniversity of Tripoli2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94583Serena, Maria Soledad; Metz, German Ernesto; Lozada, María Inés; Aspitia, Carolina Gabriela; Nicolino, Edgardo Hector; et al.; First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina; University of Tripoli; Open Veterinary Journal; 8; 2; 4-2018; 131-1392226-44852218-6050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/169608info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i2.3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:12Zoai:ri.conicet.gov.ar:11336/94583instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:13.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
spellingShingle First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
Serena, Maria Soledad
ARGENTINA
DOMESTIC DOG
SUID HERPESVIRUS 1
title_short First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_full First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_fullStr First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_full_unstemmed First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
title_sort First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina
dc.creator.none.fl_str_mv Serena, Maria Soledad
Metz, German Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Hector
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustin
Quiroga, María Alejandra
Echeverria, Maria Gabriela
author Serena, Maria Soledad
author_facet Serena, Maria Soledad
Metz, German Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Hector
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustin
Quiroga, María Alejandra
Echeverria, Maria Gabriela
author_role author
author2 Metz, German Ernesto
Lozada, María Inés
Aspitia, Carolina Gabriela
Nicolino, Edgardo Hector
Pidone, Claudio Luis
Fossaroli, Melisa Gisele
Balsalobre, Agustin
Quiroga, María Alejandra
Echeverria, Maria Gabriela
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
DOMESTIC DOG
SUID HERPESVIRUS 1
topic ARGENTINA
DOMESTIC DOG
SUID HERPESVIRUS 1
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Fil: Lozada, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina
Fil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina
Fil: Nicolino, Edgardo Hector. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; Argentina
Fil: Pidone, Claudio Luis. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de Enfermedades Infecciosas; Argentina
Fil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; Argentina
Fil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quiroga, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Departamento de patología. ; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
description Since Aujeszky's disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94583
Serena, Maria Soledad; Metz, German Ernesto; Lozada, María Inés; Aspitia, Carolina Gabriela; Nicolino, Edgardo Hector; et al.; First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina; University of Tripoli; Open Veterinary Journal; 8; 2; 4-2018; 131-139
2226-4485
2218-6050
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94583
identifier_str_mv Serena, Maria Soledad; Metz, German Ernesto; Lozada, María Inés; Aspitia, Carolina Gabriela; Nicolino, Edgardo Hector; et al.; First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina; University of Tripoli; Open Veterinary Journal; 8; 2; 4-2018; 131-139
2226-4485
2218-6050
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/169608
info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v8i2.3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Tripoli
publisher.none.fl_str_mv University of Tripoli
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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